Glass fiber sheet on a chip: For rapid, low-cost, and contamination-free quantitative immunoassay

Yuriko Oyama, Toshihisa Osaki, Koki Kamiya, Ryuji Kawano, Tsutomu Honjoh, Haruki Shibata, Toru Ide, Shoji Takeuchi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This paper describes a quantitative immunoassay chip harnessing EOF (electroosmotic flow) in glass fiber sheet. The chip consists of a glass fiber sheet-based channel covered with PMMA (polymethylmethacrylate) frames and antibody-immobilized microbeads at the observation spot (Fig. 1). Applying EOF, another labeled-antibody and rinsing buffer sequentially migrate through the antigen-antibody complex on the beads and stain the beads depending on the sample (antigen) concentration. We demonstrated a quantitative assay of insulin and succeeded in determining the concentration in 20 min. The developed chip would provide a rapid, low cost and contamination-free assay system.

Original languageEnglish
Title of host publicationProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
PublisherChemical and Biological Microsystems Society
Pages1957-1959
Number of pages3
ISBN (Print)9780979806452
Publication statusPublished - Jan 1 2012
Externally publishedYes
Event16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 - Okinawa, Japan
Duration: Oct 28 2012Nov 1 2012

Publication series

NameProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012

Other

Other16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
CountryJapan
CityOkinawa
Period10/28/1211/1/12

Keywords

  • Electroosmotic flow
  • Glass fiber sheet
  • Immunoassay

ASJC Scopus subject areas

  • Bioengineering
  • Chemical Engineering (miscellaneous)

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