Glass fiber sheet on a chip: For rapid, low-cost, and contamination-free quantitative immunoassay

Yuriko Oyama, Toshihisa Osaki, Koki Kamiya, Ryuji Kawano, Tsutomu Honjoh, Haruki Shibata, Toru Ide, Shoji Takeuchi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This paper describes a quantitative immunoassay chip harnessing EOF (electroosmotic flow) in glass fiber sheet. The chip consists of a glass fiber sheet-based channel covered with PMMA (polymethylmethacrylate) frames and antibody-immobilized microbeads at the observation spot (Fig. 1). Applying EOF, another labeled-antibody and rinsing buffer sequentially migrate through the antigen-antibody complex on the beads and stain the beads depending on the sample (antigen) concentration. We demonstrated a quantitative assay of insulin and succeeded in determining the concentration in 20 min. The developed chip would provide a rapid, low cost and contamination-free assay system.

Original languageEnglish
Title of host publicationProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
PublisherChemical and Biological Microsystems Society
Pages1957-1959
Number of pages3
ISBN (Print)9780979806452
Publication statusPublished - 2012
Externally publishedYes
Event16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 - Okinawa, Japan
Duration: Oct 28 2012Nov 1 2012

Other

Other16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
CountryJapan
CityOkinawa
Period10/28/1211/1/12

Fingerprint

Antibodies
Glass fibers
Assays
Contamination
Antigens
Immobilized Antibodies
Polymethyl Methacrylate
Antigen-Antibody Complex
Costs
Buffers
Coloring Agents
Insulin
fiberglass

Keywords

  • Electroosmotic flow
  • Glass fiber sheet
  • Immunoassay

ASJC Scopus subject areas

  • Bioengineering
  • Chemical Engineering (miscellaneous)

Cite this

Oyama, Y., Osaki, T., Kamiya, K., Kawano, R., Honjoh, T., Shibata, H., ... Takeuchi, S. (2012). Glass fiber sheet on a chip: For rapid, low-cost, and contamination-free quantitative immunoassay. In Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 (pp. 1957-1959). Chemical and Biological Microsystems Society.

Glass fiber sheet on a chip : For rapid, low-cost, and contamination-free quantitative immunoassay. / Oyama, Yuriko; Osaki, Toshihisa; Kamiya, Koki; Kawano, Ryuji; Honjoh, Tsutomu; Shibata, Haruki; Ide, Toru; Takeuchi, Shoji.

Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society, 2012. p. 1957-1959.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Oyama, Y, Osaki, T, Kamiya, K, Kawano, R, Honjoh, T, Shibata, H, Ide, T & Takeuchi, S 2012, Glass fiber sheet on a chip: For rapid, low-cost, and contamination-free quantitative immunoassay. in Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society, pp. 1957-1959, 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012, Okinawa, Japan, 10/28/12.
Oyama Y, Osaki T, Kamiya K, Kawano R, Honjoh T, Shibata H et al. Glass fiber sheet on a chip: For rapid, low-cost, and contamination-free quantitative immunoassay. In Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society. 2012. p. 1957-1959
Oyama, Yuriko ; Osaki, Toshihisa ; Kamiya, Koki ; Kawano, Ryuji ; Honjoh, Tsutomu ; Shibata, Haruki ; Ide, Toru ; Takeuchi, Shoji. / Glass fiber sheet on a chip : For rapid, low-cost, and contamination-free quantitative immunoassay. Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012. Chemical and Biological Microsystems Society, 2012. pp. 1957-1959
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