Ginkgo biloba extract (EGb 761): Inhibitory effect on nitric oxide duction in the macrophage cell line RAW 264.7

Hirotsugu Kobuchi, Marie Therese Droy-Lefaix, Yves Christen, Lester Packer

Research output: Contribution to journalArticle

150 Citations (Scopus)

Abstract

The present study was conducted to evaluate the effect of Ginkgo biloba extract (EGb 761) on the synthesis of nitric oxide (NO) induced by lipopolysaccharide (LPS) plus interferon-γ (IFN-γ) in the mouse macrophage cell line RAW 264.7. EGb 761 inhibited nitrite and nitrate production, taken as an index for NO, in a concentration-dependent fashion. The IC50 for inhibition of nitrite production by activated macrophages was about 100 μg/mL EGb 761. The inducible NO synthase (iNOS) enzyme activity of cytosolic preparations from activated RAW 264.7 cells was inhibited by treatment with EGb 761. In addition, reverse transcription-polymerase chain reaction (RT-PCR) analysis revealed that the expression of iNOS mRNA in activated macrophages was suppressed by high concentrations of EGb 761. However, NF-κB DNA binding activity induced by activation with LPS/IFN-γ was not inhibited by EGb 761. These findings indicate that not only does EGb 761 directly act as an NO scavenger but also that it inhibits NO production in LPS/IFN-γ-activated macrophages by concomitant inhibition of induction of iNOS mRNA and the enzyme activity of iNOS. Thus, EGb 761 may act as a potent inhibitor of NO production under tissue-damaging inflammatory conditions.

Original languageEnglish
Pages (from-to)897-903
Number of pages7
JournalBiochemical Pharmacology
Volume53
Issue number6
DOIs
Publication statusPublished - Mar 21 1997
Externally publishedYes

Fingerprint

Macrophages
Nitric Oxide
Cells
Nitric Oxide Synthase
Interferons
Lipopolysaccharides
Enzyme activity
Nitrites
Enzyme inhibition
Messenger RNA
Ginkgo biloba extract 761
RAW 264.7 Cells
Polymerase chain reaction
Nitric Oxide Synthase Type II
Transcription
Nitrates
Enzymes
Inhibitory Concentration 50
Reverse Transcription
Chemical activation

Keywords

  • Egb 761
  • Ginkgo biloba extract
  • inducible nitric oxide synthase
  • macrophages flavonoids
  • nitric oxide

ASJC Scopus subject areas

  • Pharmacology

Cite this

Ginkgo biloba extract (EGb 761) : Inhibitory effect on nitric oxide duction in the macrophage cell line RAW 264.7. / Kobuchi, Hirotsugu; Droy-Lefaix, Marie Therese; Christen, Yves; Packer, Lester.

In: Biochemical Pharmacology, Vol. 53, No. 6, 21.03.1997, p. 897-903.

Research output: Contribution to journalArticle

Kobuchi, Hirotsugu ; Droy-Lefaix, Marie Therese ; Christen, Yves ; Packer, Lester. / Ginkgo biloba extract (EGb 761) : Inhibitory effect on nitric oxide duction in the macrophage cell line RAW 264.7. In: Biochemical Pharmacology. 1997 ; Vol. 53, No. 6. pp. 897-903.
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