Gingivae contain neural-crest- and mesoderm-derived mesenchymal stem cells

X. Xu, C. Chen, Kentaro Akiyama, Y. Chai, A. D. Le, Z. Wang, S. Shi

Research output: Contribution to journalArticle

77 Citations (Scopus)

Abstract

Gingivae represent a unique soft tissue that serves as a biological barrier to cover the oral cavity side of the maxilla and mandible. Recently, the gingivae were identified as containing mesenchymal stem cells (GMSCs). However, it is unknown whether the GMSCs are derived from cranial neural crest cells (CNCC) or the mesoderm. In this study, we show that around 90% of GMSCs are derived from CNCC and 10% from the mesoderm. In comparison with mesoderm MSCs (M-GMSCs), CNCC-derived GMSCs (N-GMSCs) show an elevated capacity to differentiate into neural cells and chondrocytes and induce activated T-cell apoptosis in vitro. When transplanted into mice with dextran sulfate sodium (DSS)-induced colitis, N-GMSCs showed superior effects in ameliorating inflammatory-related disease phenotype in comparison with the M-GMSC treatment group. Mechanistically, the increased immunomodulatory effect of N-GMSCs is associated with up-regulated expression of FAS ligand (FASL), a transmembrane protein that plays an important role in MSC-based immunomodulation. In summary, our study indicates that the gingivae contain both neural-crest- and mesoderm-derived MSCs with distinctive stem cell properties.

Original languageEnglish
Pages (from-to)825-832
Number of pages8
JournalJournal of Dental Research
Volume92
Issue number9
DOIs
Publication statusPublished - Sep 2013
Externally publishedYes

Fingerprint

Neural Crest
Gingiva
Mesoderm
Mesenchymal Stromal Cells
Dextran Sulfate
Immunomodulation
Maxilla
Colitis
Chondrocytes
Mandible
Mouth
Stem Cells
Apoptosis
Ligands
T-Lymphocytes
Phenotype
Proteins
Therapeutics

Keywords

  • apoptosis
  • differentiation
  • gingiva
  • immunomodulation
  • mesenchymal stem cells
  • neural crest cells

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Gingivae contain neural-crest- and mesoderm-derived mesenchymal stem cells. / Xu, X.; Chen, C.; Akiyama, Kentaro; Chai, Y.; Le, A. D.; Wang, Z.; Shi, S.

In: Journal of Dental Research, Vol. 92, No. 9, 09.2013, p. 825-832.

Research output: Contribution to journalArticle

Xu, X. ; Chen, C. ; Akiyama, Kentaro ; Chai, Y. ; Le, A. D. ; Wang, Z. ; Shi, S. / Gingivae contain neural-crest- and mesoderm-derived mesenchymal stem cells. In: Journal of Dental Research. 2013 ; Vol. 92, No. 9. pp. 825-832.
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