Gibberellic acid alleviates cadmium toxicity by reducing nitric oxide accumulation and expression of IRT1 in Arabidopsis thaliana

Xiao Fang Zhu, Tao Jiang, Zhi Wei Wang, Guijie Lei, Yuan Zhi Shi, Gui Xin Li, Shao Jian Zheng

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

Gibberellic acid (GA) is involved in not only plant growth and development but also plant responses to abiotic stresses. Here it was found that treating the plants with GA concentrations from 0.1 to 5μM for 24h had no obvious effect on root elongation in the absence of cadmium (Cd), whereas in the presence of Cd2+, GA at 5μM improved root growth, reduced Cd content and lipid peroxidation in the roots, indicating that GA can partially alleviate Cd toxicity. Cd2+ increased nitric oxide (NO) accumulation in the roots, but GA remarkably reduced it, and suppressed the up-regulation of the expression of IRT1. In contrary, the beneficial effect of GA on alleviating Cd toxicity was not observed in an IRT1 knock-out mutant irt1, suggesting the involvement of IRT1 in Cd2+ absorption. Furthermore, the GA-induced reduction of NO and Cd content can also be partially reversed by the application of a NO donor (S-nitrosoglutathione [GSNO]). Taken all these together, the results showed that GA-alleviated Cd toxicity is mediated through the reduction of the Cd-dependent NO accumulation and expression of Cd2+ uptake related gene-IRT1 in Arabidopsis.

Original languageEnglish
Pages (from-to)302-307
Number of pages6
JournalJournal of Hazardous Materials
Volume239-240
DOIs
Publication statusPublished - Nov 15 2012
Externally publishedYes

Fingerprint

Nitric oxide
nitric oxide
Cadmium
Arabidopsis
Toxicity
Nitric Oxide
cadmium
toxicity
Acids
acid
S-Nitrosoglutathione
Plant Development
Nitric Oxide Donors
gibberellic acid
Growth and Development
growth and development
Lipids
Lipid Peroxidation
Elongation
Up-Regulation

Keywords

  • Arabidopsis
  • Cd stress
  • Exogenous GA
  • Gene expression
  • NO fluorescence
  • Root elongation
  • Transport

ASJC Scopus subject areas

  • Environmental Engineering
  • Environmental Chemistry
  • Waste Management and Disposal
  • Pollution
  • Health, Toxicology and Mutagenesis

Cite this

Gibberellic acid alleviates cadmium toxicity by reducing nitric oxide accumulation and expression of IRT1 in Arabidopsis thaliana. / Zhu, Xiao Fang; Jiang, Tao; Wang, Zhi Wei; Lei, Guijie; Shi, Yuan Zhi; Li, Gui Xin; Zheng, Shao Jian.

In: Journal of Hazardous Materials, Vol. 239-240, 15.11.2012, p. 302-307.

Research output: Contribution to journalArticle

Zhu, Xiao Fang ; Jiang, Tao ; Wang, Zhi Wei ; Lei, Guijie ; Shi, Yuan Zhi ; Li, Gui Xin ; Zheng, Shao Jian. / Gibberellic acid alleviates cadmium toxicity by reducing nitric oxide accumulation and expression of IRT1 in Arabidopsis thaliana. In: Journal of Hazardous Materials. 2012 ; Vol. 239-240. pp. 302-307.
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