Abstract
The healing of an injured musculoskeletal system requires an influx of mesenchymal cells that can differentiate into osteoblasts, fibroblasts, chondroblasts, and skeletal myoblasts. However, whether these mesenchymal cells arise from the circulation (bone marrow) or the injured tissues themselves has been controversial. To reveal the spatiotemporal characteristics of the reparative mesenchymal cells, we investigated the healing process after patellar tendon injury using two types of green fluorescent protein (GFP) chimeric rats; one expressing GFP in the circulating cells, and the other expressing it in the patellar tendon. We analyzed the behavior of GFP-positive cells after experimental tendon injury in both chimeric rats to clarify the origin of reparative cells. At 24 h after the injury, the wound contained circulation-derived cells but not tendon-derived cells. Tendon-derived cells first appeared in the wounded area at 3 days after the injury, and had significantly increased in number with time and had maintained a high level of proliferative activity until 7 days after the injury, whereas the circulation-derived cells had decreased in number and had been replaced by the tendon-derived cells. These findings suggest that circulation-derived and tendon-derived cells contribute to the healing of tendons in different periods as part of a biphasic process.
| Original language | English |
|---|---|
| Pages (from-to) | 684-691 |
| Number of pages | 8 |
| Journal | Journal of Cellular Physiology |
| Volume | 210 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Mar 2007 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Clinical Biochemistry
- Cell Biology
- Physiology
Cite this
GFP chimeric models exhibited a biphasic pattern of mesenchymal cell invasion in tendon healing. / Kajikawa, Yoshiteru; Morihara, Toru; Watanabe, Nobuyoshi; Sakamoto, Hirotaka; Matsuda, Ken Ichi; Kobayashi, Masashi; Oshima, Yasushi; Yoshida, Atsuhiko; Kawata, Mitsuhiro; Kubo, Toshikazu.
In: Journal of Cellular Physiology, Vol. 210, No. 3, 03.2007, p. 684-691.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - GFP chimeric models exhibited a biphasic pattern of mesenchymal cell invasion in tendon healing
AU - Kajikawa, Yoshiteru
AU - Morihara, Toru
AU - Watanabe, Nobuyoshi
AU - Sakamoto, Hirotaka
AU - Matsuda, Ken Ichi
AU - Kobayashi, Masashi
AU - Oshima, Yasushi
AU - Yoshida, Atsuhiko
AU - Kawata, Mitsuhiro
AU - Kubo, Toshikazu
PY - 2007/3
Y1 - 2007/3
N2 - The healing of an injured musculoskeletal system requires an influx of mesenchymal cells that can differentiate into osteoblasts, fibroblasts, chondroblasts, and skeletal myoblasts. However, whether these mesenchymal cells arise from the circulation (bone marrow) or the injured tissues themselves has been controversial. To reveal the spatiotemporal characteristics of the reparative mesenchymal cells, we investigated the healing process after patellar tendon injury using two types of green fluorescent protein (GFP) chimeric rats; one expressing GFP in the circulating cells, and the other expressing it in the patellar tendon. We analyzed the behavior of GFP-positive cells after experimental tendon injury in both chimeric rats to clarify the origin of reparative cells. At 24 h after the injury, the wound contained circulation-derived cells but not tendon-derived cells. Tendon-derived cells first appeared in the wounded area at 3 days after the injury, and had significantly increased in number with time and had maintained a high level of proliferative activity until 7 days after the injury, whereas the circulation-derived cells had decreased in number and had been replaced by the tendon-derived cells. These findings suggest that circulation-derived and tendon-derived cells contribute to the healing of tendons in different periods as part of a biphasic process.
AB - The healing of an injured musculoskeletal system requires an influx of mesenchymal cells that can differentiate into osteoblasts, fibroblasts, chondroblasts, and skeletal myoblasts. However, whether these mesenchymal cells arise from the circulation (bone marrow) or the injured tissues themselves has been controversial. To reveal the spatiotemporal characteristics of the reparative mesenchymal cells, we investigated the healing process after patellar tendon injury using two types of green fluorescent protein (GFP) chimeric rats; one expressing GFP in the circulating cells, and the other expressing it in the patellar tendon. We analyzed the behavior of GFP-positive cells after experimental tendon injury in both chimeric rats to clarify the origin of reparative cells. At 24 h after the injury, the wound contained circulation-derived cells but not tendon-derived cells. Tendon-derived cells first appeared in the wounded area at 3 days after the injury, and had significantly increased in number with time and had maintained a high level of proliferative activity until 7 days after the injury, whereas the circulation-derived cells had decreased in number and had been replaced by the tendon-derived cells. These findings suggest that circulation-derived and tendon-derived cells contribute to the healing of tendons in different periods as part of a biphasic process.
UR - http://www.scopus.com/inward/record.url?scp=33846635486&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33846635486&partnerID=8YFLogxK
U2 - 10.1002/jcp.20876
DO - 10.1002/jcp.20876
M3 - Article
C2 - 17154365
AN - SCOPUS:33846635486
VL - 210
SP - 684
EP - 691
JO - Journal of Cellular Physiology
JF - Journal of Cellular Physiology
SN - 0021-9541
IS - 3
ER -