Gestational changes in production of NO and expression of NOS mRNA isoforms in the rat placenta

Takehito Suzuki, Yoshinori Ikeda, Hiroshi Yoshikawa, Kazuaki Tanaka, Hidetoshi Morita, Masako Yamamoto, Tatsuya Takizawa

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

To monitor changes in NO production over time in the fetal placenta of rats, we used electron paramagnetic resonance spectroscopy with the Fe-N-(dithiocarboxy) sarcosine (Fe-DTCS) complex as an NO-trapping reagent. The expression of nitric oxide synthase (NOS) isoforms was examined in parallel using quantitative RT-PCR. NO production was first detected on day 13.5 of gestation. NO levels reached a peak on day 15.5, then decreased significantly during the last few days of gestation. The pattern of expression of NOS II mRNA was in good agreement with changes in NO levels, whereas NOS III mRNA expression did not change markedly during gestation. Thus, it appears that NO levels in the placenta are NOS II-dependent and differ at different gestational stages.

Original languageEnglish
Pages (from-to)495-498
Number of pages4
JournalJournal of Veterinary Medical Science
Volume71
Issue number4
DOIs
Publication statusPublished - May 2 2009
Externally publishedYes

Fingerprint

RNA Isoforms
nitric oxide synthase
placenta
Nitric Oxide Synthase
Placenta
Nitric Oxide Synthase Type II
Pregnancy
rats
pregnancy
Messenger RNA
Electron Spin Resonance Spectroscopy
Spectrum Analysis
electron paramagnetic resonance spectroscopy
Protein Isoforms
trapping
Polymerase Chain Reaction
reverse transcriptase polymerase chain reaction

Keywords

  • Electron paramagnetic resonance (EPR)
  • Fetal placenta
  • Nitric oxide

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Gestational changes in production of NO and expression of NOS mRNA isoforms in the rat placenta. / Suzuki, Takehito; Ikeda, Yoshinori; Yoshikawa, Hiroshi; Tanaka, Kazuaki; Morita, Hidetoshi; Yamamoto, Masako; Takizawa, Tatsuya.

In: Journal of Veterinary Medical Science, Vol. 71, No. 4, 02.05.2009, p. 495-498.

Research output: Contribution to journalArticle

Suzuki, Takehito ; Ikeda, Yoshinori ; Yoshikawa, Hiroshi ; Tanaka, Kazuaki ; Morita, Hidetoshi ; Yamamoto, Masako ; Takizawa, Tatsuya. / Gestational changes in production of NO and expression of NOS mRNA isoforms in the rat placenta. In: Journal of Veterinary Medical Science. 2009 ; Vol. 71, No. 4. pp. 495-498.
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