Genotypic difference in silicon uptake and expression of silicon transporter genes in rice

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67 Citations (Scopus)

Abstract

Rice (Oryza sativa) is a highly silicon (Si)-accumulating species that shows genotypic differences in Si accumulation. We investigated the physiological and molecular mechanisms involved in the genotypic difference in Si uptake between the japonica var. Nipponbare and the indica var. Kasalath. Both the Si concentration in the shoot and the Si uptake per root dry weight were higher in Nipponbare than in Kasalath grown in either soil or nutrient solution. The Si uptake by a single root was also higher in Nipponbare than in Kasalath. A kinetics study showed that Nipponbare and Kasalath had a similar Km value, whereas the Vmax was higher in Nipponbare. The expression of two Si transporter genes (Low silicon rice 1 [Lsi1] and Lsi2) investigated using real-time reverse transcription polymerase chain reaction revealed higher expression of both genes in Nipponbare than in Kasalath. Immunostaining with Lsi1 and Lsi2 antibodies revealed a similar pattern of subcellular localization of these two Si transporters in both varieties; Lsi1 and Lsi2 were localized at the distal and proximal sides, respectively, of both exodermis and endodermis of the roots. These results revealed that the genotypic difference in the Si accumulation results from the difference in abundance of Si transporters in rice roots.

Original languageEnglish
Pages (from-to)919-924
Number of pages6
JournalPlant Physiology
Volume145
Issue number3
DOIs
Publication statusPublished - Nov 2007

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Silicon
silicon
transporters
rice
Genes
genes
Oryza
endodermis
soil solution
Reverse Transcription
nutrient solutions
Oryza sativa
quantitative polymerase chain reaction
Soil
reverse transcriptase polymerase chain reaction

ASJC Scopus subject areas

  • Plant Science

Cite this

Genotypic difference in silicon uptake and expression of silicon transporter genes in rice. / Ma, Jian Feng; Yamaji, Naoki; Tamai, Kazunori; Mitani, Namiki.

In: Plant Physiology, Vol. 145, No. 3, 11.2007, p. 919-924.

Research output: Contribution to journalArticle

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