Genotoxic stress induces Sca-1-expressing metastatic mammary cancer cells

Jianlin Gong, Benjamin J. Lang, Desheng Weng, Takanori Eguchi, Ayesha Murshid, Thiago J. Borges, Sachin Doshi, Baizheng Song, Mary A. Stevenson, Stuart K. Calderwood

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)


We describe a cell damage-induced phenotype in mammary carcinoma cells involving acquisition of enhanced migratory and metastatic properties. Induction of this state by radiation required increased activity of the Ptgs2 gene product cyclooxygenase 2 (Cox2), secretion of its bioactive lipid product prostaglandin E2 (PGE2), and the activity of the PGE2 receptor EP4. Although largely transient, decaying to low levels in a few days to a week, this phenotype was cumulative with damage and levels of cell markers Sca-1 and ALDH1 increased with treatment dose. The Sca-1 + , metastatic phenotype was inhibited by both Cox2 inhibitors and PGE2 receptor antagonists, suggesting novel approaches to radiosensitization.

Original languageEnglish
Pages (from-to)1249-1263
Number of pages15
JournalMolecular Oncology
Issue number8
Publication statusPublished - Aug 2018
Externally publishedYes


  • Sca-1
  • genotoxic stress
  • radiation bystander effect
  • radiation therapy
  • responses to cancer therapy

ASJC Scopus subject areas

  • Molecular Medicine
  • Genetics
  • Oncology
  • Cancer Research


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