Abstract
Here we report the genomic structure including the promoter sequence and coding region of NtPDR1 (Nicotiana tabacum Pleiotropic Drug Resistance 1), which is an elicitor-responsive gene encoding an ATP binding cassette (ABC) transporter that might be involved in the defense response in tobacco, as we reported recently. The NtPDR1 gene consists of 20 exons and 19 introns. Among the introns, the first and fifth are much larger than the others and harbor typical miniature inverted-repeat transposable elements (MITEs). One of the MITE elements in the first intron, termed NtToya1, belongs to the Toya family that was recently described in rice, while the other element in the fifth intron, termed NtStowaway101, shows high homology with the Stowaway elements of the IS630-Tc1-mariner family. Many of the genes we found to harbor Toya and Stowaway elements in Nicotiana species by BLAST search are also involved in stress responses or plant-pathogen interactions. The existence of putative cis-elements (a GCC box, three W boxes, and several JA-responsive elements) in the promoter region supports our previous finding that this gene is strongly inducible by elicitation and methyljasmonate, and that this ABC transporter might be essential for plant defense responses. Furthermore, Southern blot analysis and PCR amplification of the introns harboring the MITE-like elements from genomic DNA of three Nicotiana species suggests that NtPDR1 originated from N. sylvestris.
| Original language | English |
|---|---|
| Pages (from-to) | 409-418 |
| Number of pages | 10 |
| Journal | Genes and Genetic Systems |
| Volume | 78 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - Dec 2003 |
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Keywords
- ABC transporter
- Genomic structure
- MITE
- Nicotiana tabacum
- NtPDR1
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- Biochemistry, Genetics and Molecular Biology(all)
- Biochemistry
- Genetics
- Genetics(clinical)
Cite this
Genomic structure of the NtPDR1 gene, harboring the two miniature inverted-repeat transposable elements, NtToya1 and NtStowaway101. / Schenke, Dirk; Sasabe, Michiko; Toyoda, Kazuhiro; Inagaki, Yoshi Shige; Shiraishi, Tomonori; Ichinose, Yuki.
In: Genes and Genetic Systems, Vol. 78, No. 6, 12.2003, p. 409-418.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Genomic structure of the NtPDR1 gene, harboring the two miniature inverted-repeat transposable elements, NtToya1 and NtStowaway101
AU - Schenke, Dirk
AU - Sasabe, Michiko
AU - Toyoda, Kazuhiro
AU - Inagaki, Yoshi Shige
AU - Shiraishi, Tomonori
AU - Ichinose, Yuki
PY - 2003/12
Y1 - 2003/12
N2 - Here we report the genomic structure including the promoter sequence and coding region of NtPDR1 (Nicotiana tabacum Pleiotropic Drug Resistance 1), which is an elicitor-responsive gene encoding an ATP binding cassette (ABC) transporter that might be involved in the defense response in tobacco, as we reported recently. The NtPDR1 gene consists of 20 exons and 19 introns. Among the introns, the first and fifth are much larger than the others and harbor typical miniature inverted-repeat transposable elements (MITEs). One of the MITE elements in the first intron, termed NtToya1, belongs to the Toya family that was recently described in rice, while the other element in the fifth intron, termed NtStowaway101, shows high homology with the Stowaway elements of the IS630-Tc1-mariner family. Many of the genes we found to harbor Toya and Stowaway elements in Nicotiana species by BLAST search are also involved in stress responses or plant-pathogen interactions. The existence of putative cis-elements (a GCC box, three W boxes, and several JA-responsive elements) in the promoter region supports our previous finding that this gene is strongly inducible by elicitation and methyljasmonate, and that this ABC transporter might be essential for plant defense responses. Furthermore, Southern blot analysis and PCR amplification of the introns harboring the MITE-like elements from genomic DNA of three Nicotiana species suggests that NtPDR1 originated from N. sylvestris.
AB - Here we report the genomic structure including the promoter sequence and coding region of NtPDR1 (Nicotiana tabacum Pleiotropic Drug Resistance 1), which is an elicitor-responsive gene encoding an ATP binding cassette (ABC) transporter that might be involved in the defense response in tobacco, as we reported recently. The NtPDR1 gene consists of 20 exons and 19 introns. Among the introns, the first and fifth are much larger than the others and harbor typical miniature inverted-repeat transposable elements (MITEs). One of the MITE elements in the first intron, termed NtToya1, belongs to the Toya family that was recently described in rice, while the other element in the fifth intron, termed NtStowaway101, shows high homology with the Stowaway elements of the IS630-Tc1-mariner family. Many of the genes we found to harbor Toya and Stowaway elements in Nicotiana species by BLAST search are also involved in stress responses or plant-pathogen interactions. The existence of putative cis-elements (a GCC box, three W boxes, and several JA-responsive elements) in the promoter region supports our previous finding that this gene is strongly inducible by elicitation and methyljasmonate, and that this ABC transporter might be essential for plant defense responses. Furthermore, Southern blot analysis and PCR amplification of the introns harboring the MITE-like elements from genomic DNA of three Nicotiana species suggests that NtPDR1 originated from N. sylvestris.
KW - ABC transporter
KW - Genomic structure
KW - MITE
KW - Nicotiana tabacum
KW - NtPDR1
UR - http://www.scopus.com/inward/record.url?scp=1542378140&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=1542378140&partnerID=8YFLogxK
U2 - 10.1266/ggs.78.409
DO - 10.1266/ggs.78.409
M3 - Article
C2 - 14973342
AN - SCOPUS:1542378140
VL - 78
SP - 409
EP - 418
JO - Genes and Genetic Systems
JF - Genes and Genetic Systems
SN - 1341-7568
IS - 6
ER -