Genomic characterization of antibiotic resistance-encoding genes in clinical isolates of Vibrio cholerae non-O1/non-O139 strains from Kolkata, India: generation of novel types of genomic islands containing plural antibiotic resistance genes

Daichi Morita, Eizo Takahashi, Masatomo Morita, Makoto Ohnishi, Tamaki Mizuno, Shin ichi Miyoshi, Devarati Dutta, Thandavarayan Ramamurthy, Goutam Chowdhury, Asish K. Mukhopadhyay, Keinosuke Okamoto

Research output: Contribution to journalArticle

Abstract

Non-O1/non-O139 nontoxigenic Vibrio cholerae associated with cholera-like diarrhea has been reported in Kolkata, India. However, the property involved in the pathogenicity of these strains has remained unclear. The character of 25 non-O1/non-O139 nontoxigenic V. cholerae isolated during 8 years from 2007 to 2014 in Kolkata was examined. Determination of the serogroup showed that the serogroups O6, O10, O35, O36, O39, and O70 were represented by two strains in each serogroup, and the remaining isolates belonged to different serogroups. To clarify the character of antibiotic resistance of these isolates, an antibiotic resistance test and the gene analysis were performed. According to antimicrobial drug susceptibility testing, 13 strains were classified as drug resistant. Among them, 10 strains were quinolone resistant and 6 of the 13 strains were resistant to more than three antibiotics. To define the genetic background of the antibiotic character of these strains, whole-genome sequences of these strains were determined. From the analysis of these sequences, it becomes clear that all quinolone resistance isolates have mutations in quinolone resistance-determining regions. Further research on the genome sequence showed that four strains possess Class 1 integrons in their genomes, and that three of the four integrons are found to be located in their genomic islands. These genomic islands are novel types. This indicates that various integrons containing drug resistance genes are spreading among V. cholerae non-O1/non-O139 strains through the action of newly generated genomic islands.

Original languageEnglish
Pages (from-to)435-444
Number of pages10
JournalMICROBIOLOGY and IMMUNOLOGY
Volume64
Issue number6
DOIs
Publication statusPublished - Jun 1 2020

Keywords

  • Vibrio cholerae
  • antibiotic resistance
  • diarrhea
  • genome sequence
  • genomic island
  • integron

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Virology

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