Genome-wide survey and expression analysis of the plant-specific NAC transcription factor family in soybean during development and dehydration stress

Dung Tien Le, Rie Nishiyama, Yasuko Watanabe, Keiichi Mochida, Kazuko Yamaguchi-Shinozaki, Kazuo Shinozaki, Lam Son Phan Tran

Research output: Contribution to journalArticle

208 Citations (Scopus)

Abstract

Plant-specific NAC transcription factors (TFs) play important roles in regulating diverse biological processes, including development, senescence, growth, cell division and responses to environmental stress stimuli. Within the soybean genome, we identified 152 full-length GmNAC TFs, including 11 membrane-bound members. In silico analysis of the GmNACs, together with their Arabidopsis and rice counterparts, revealed similar NAC architecture. Next, we explored the soybean Affymetrix array and Illumina transcriptome sequence data to analyse tissue-specific expression profiles of GmNAC genes. Phylogenetic analysis using stress-related NAC TFs from Arabidopsis and rice as seeding sequences identified 58 of the 152 GmNACs as putative stress-responsive genes, including eight previously reported dehydration-responsive GmNACs. We could design gene-specific primers for quantitative real-time PCR verification of 38 out of 50 newly predicted stress-related genes. Twenty-five and six GmNACs were found to be induced and repressed 2-fold or more, respectively, in soybean roots and/or shoots in response to dehydration. GmNAC085, whose amino acid sequence was 39; identical to that of well-known SNAC1/ONAC2, was the most induced gene upon dehydration, showing 390-fold and 20-fold induction in shoots and roots, respectively. Our systematic analysis has identified excellent tissue-specific and/or dehydration-responsive candidate GmNAC genes for in-depth characterization and future development of improved drought-tolerant transgenic soybeans.

Original languageEnglish
Pages (from-to)263-276
Number of pages14
JournalDNA Research
Volume18
Issue number4
DOIs
Publication statusPublished - Aug 2011
Externally publishedYes

Fingerprint

Dehydration
Soybeans
Transcription Factors
Genome
Genes
Transcriptome
Arabidopsis
Biological Phenomena
Droughts
Growth and Development
Cell Division
Computer Simulation
Real-Time Polymerase Chain Reaction
Amino Acid Sequence
Surveys and Questionnaires
Membranes
Oryza

Keywords

  • dehydration
  • expression analysis
  • NAC transcription factors
  • sequence analysis
  • soybean

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology

Cite this

Genome-wide survey and expression analysis of the plant-specific NAC transcription factor family in soybean during development and dehydration stress. / Le, Dung Tien; Nishiyama, Rie; Watanabe, Yasuko; Mochida, Keiichi; Yamaguchi-Shinozaki, Kazuko; Shinozaki, Kazuo; Tran, Lam Son Phan.

In: DNA Research, Vol. 18, No. 4, 08.2011, p. 263-276.

Research output: Contribution to journalArticle

Le, Dung Tien ; Nishiyama, Rie ; Watanabe, Yasuko ; Mochida, Keiichi ; Yamaguchi-Shinozaki, Kazuko ; Shinozaki, Kazuo ; Tran, Lam Son Phan. / Genome-wide survey and expression analysis of the plant-specific NAC transcription factor family in soybean during development and dehydration stress. In: DNA Research. 2011 ; Vol. 18, No. 4. pp. 263-276.
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