Abstract
Escherichia coli K-12 has the ability to migrate on semisolid media by means of swarming motility. A systematic and comprehensive collection of gene-disrupted E. coli K-12 mutants (the Keio collection) was used to identify the genes involved in the swarming motility of this bacterium. Of the 3,985 nonessential gene mutants, 294 were found to exhibit a strongly repressed-swarming phenotype. Further, 216 of the 294 mutants displayed no significant defects in swimming motility; therefore, the 216 genes were considered to be specifically associated with the swarming phenotype. The swarming-associated genes were classified into various functional categories, indicating that swarming is a specialized form of motility that requires a wide variety of cellular activities. These genes include genes for tricarboxylic acid cycle and glucose metabolism, iron acquisition, chaperones and protein-folding catalysts, signal transduction, and biosynthesis of cell surface components, such as lipopolysaccharide, the enterobacterial common antigen, and type 1 fimbriae. Lipopolysaccharide and the enterobacterial common antigen may be important surface-acting components that contribute to the reduction of surface tension, thereby facilitating the swarm migration in the E. coli K-12 strain.
| Original language | English |
|---|---|
| Pages (from-to) | 950-957 |
| Number of pages | 8 |
| Journal | Journal of Bacteriology |
| Volume | 189 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Feb 2007 |
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ASJC Scopus subject areas
- Applied Microbiology and Biotechnology
- Immunology
Cite this
Genome-wide screening of genes required for swarming motility in Escherichia coli K-12. / Inoue, Tetsuyoshi; Shingaki, Ryuji; Hirose, Shotaro; Waki, Kaori; Mori, Hirotada; Fukui, Kazuhiro.
In: Journal of Bacteriology, Vol. 189, No. 3, 02.2007, p. 950-957.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Genome-wide screening of genes required for swarming motility in Escherichia coli K-12
AU - Inoue, Tetsuyoshi
AU - Shingaki, Ryuji
AU - Hirose, Shotaro
AU - Waki, Kaori
AU - Mori, Hirotada
AU - Fukui, Kazuhiro
PY - 2007/2
Y1 - 2007/2
N2 - Escherichia coli K-12 has the ability to migrate on semisolid media by means of swarming motility. A systematic and comprehensive collection of gene-disrupted E. coli K-12 mutants (the Keio collection) was used to identify the genes involved in the swarming motility of this bacterium. Of the 3,985 nonessential gene mutants, 294 were found to exhibit a strongly repressed-swarming phenotype. Further, 216 of the 294 mutants displayed no significant defects in swimming motility; therefore, the 216 genes were considered to be specifically associated with the swarming phenotype. The swarming-associated genes were classified into various functional categories, indicating that swarming is a specialized form of motility that requires a wide variety of cellular activities. These genes include genes for tricarboxylic acid cycle and glucose metabolism, iron acquisition, chaperones and protein-folding catalysts, signal transduction, and biosynthesis of cell surface components, such as lipopolysaccharide, the enterobacterial common antigen, and type 1 fimbriae. Lipopolysaccharide and the enterobacterial common antigen may be important surface-acting components that contribute to the reduction of surface tension, thereby facilitating the swarm migration in the E. coli K-12 strain.
AB - Escherichia coli K-12 has the ability to migrate on semisolid media by means of swarming motility. A systematic and comprehensive collection of gene-disrupted E. coli K-12 mutants (the Keio collection) was used to identify the genes involved in the swarming motility of this bacterium. Of the 3,985 nonessential gene mutants, 294 were found to exhibit a strongly repressed-swarming phenotype. Further, 216 of the 294 mutants displayed no significant defects in swimming motility; therefore, the 216 genes were considered to be specifically associated with the swarming phenotype. The swarming-associated genes were classified into various functional categories, indicating that swarming is a specialized form of motility that requires a wide variety of cellular activities. These genes include genes for tricarboxylic acid cycle and glucose metabolism, iron acquisition, chaperones and protein-folding catalysts, signal transduction, and biosynthesis of cell surface components, such as lipopolysaccharide, the enterobacterial common antigen, and type 1 fimbriae. Lipopolysaccharide and the enterobacterial common antigen may be important surface-acting components that contribute to the reduction of surface tension, thereby facilitating the swarm migration in the E. coli K-12 strain.
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UR - http://www.scopus.com/inward/citedby.url?scp=33846636385&partnerID=8YFLogxK
U2 - 10.1128/JB.01294-06
DO - 10.1128/JB.01294-06
M3 - Article
C2 - 17122336
AN - SCOPUS:33846636385
VL - 189
SP - 950
EP - 957
JO - Journal of Bacteriology
JF - Journal of Bacteriology
SN - 0021-9193
IS - 3
ER -