Genome-wide screening of genes required for swarming motility in Escherichia coli K-12

Tetsuyoshi Inoue, Ryuji Shingaki, Shotaro Hirose, Kaori Waki, Hirotada Mori, Kazuhiro Fukui

Research output: Contribution to journalArticle

114 Citations (Scopus)

Abstract

Escherichia coli K-12 has the ability to migrate on semisolid media by means of swarming motility. A systematic and comprehensive collection of gene-disrupted E. coli K-12 mutants (the Keio collection) was used to identify the genes involved in the swarming motility of this bacterium. Of the 3,985 nonessential gene mutants, 294 were found to exhibit a strongly repressed-swarming phenotype. Further, 216 of the 294 mutants displayed no significant defects in swimming motility; therefore, the 216 genes were considered to be specifically associated with the swarming phenotype. The swarming-associated genes were classified into various functional categories, indicating that swarming is a specialized form of motility that requires a wide variety of cellular activities. These genes include genes for tricarboxylic acid cycle and glucose metabolism, iron acquisition, chaperones and protein-folding catalysts, signal transduction, and biosynthesis of cell surface components, such as lipopolysaccharide, the enterobacterial common antigen, and type 1 fimbriae. Lipopolysaccharide and the enterobacterial common antigen may be important surface-acting components that contribute to the reduction of surface tension, thereby facilitating the swarm migration in the E. coli K-12 strain.

Original languageEnglish
Pages (from-to)950-957
Number of pages8
JournalJournal of Bacteriology
Volume189
Issue number3
DOIs
Publication statusPublished - Feb 2007

Fingerprint

Genome
Escherichia coli
Genes
Lipopolysaccharides
Phenotype
Surface Tension
Citric Acid Cycle
Protein Folding
Cellular Structures
Signal Transduction
Iron
Bacteria
Glucose
enterobacterial common antigen

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

Inoue, T., Shingaki, R., Hirose, S., Waki, K., Mori, H., & Fukui, K. (2007). Genome-wide screening of genes required for swarming motility in Escherichia coli K-12. Journal of Bacteriology, 189(3), 950-957. https://doi.org/10.1128/JB.01294-06

Genome-wide screening of genes required for swarming motility in Escherichia coli K-12. / Inoue, Tetsuyoshi; Shingaki, Ryuji; Hirose, Shotaro; Waki, Kaori; Mori, Hirotada; Fukui, Kazuhiro.

In: Journal of Bacteriology, Vol. 189, No. 3, 02.2007, p. 950-957.

Research output: Contribution to journalArticle

Inoue, T, Shingaki, R, Hirose, S, Waki, K, Mori, H & Fukui, K 2007, 'Genome-wide screening of genes required for swarming motility in Escherichia coli K-12', Journal of Bacteriology, vol. 189, no. 3, pp. 950-957. https://doi.org/10.1128/JB.01294-06
Inoue, Tetsuyoshi ; Shingaki, Ryuji ; Hirose, Shotaro ; Waki, Kaori ; Mori, Hirotada ; Fukui, Kazuhiro. / Genome-wide screening of genes required for swarming motility in Escherichia coli K-12. In: Journal of Bacteriology. 2007 ; Vol. 189, No. 3. pp. 950-957.
@article{747108c81cfc473785d3259b830384c6,
title = "Genome-wide screening of genes required for swarming motility in Escherichia coli K-12",
abstract = "Escherichia coli K-12 has the ability to migrate on semisolid media by means of swarming motility. A systematic and comprehensive collection of gene-disrupted E. coli K-12 mutants (the Keio collection) was used to identify the genes involved in the swarming motility of this bacterium. Of the 3,985 nonessential gene mutants, 294 were found to exhibit a strongly repressed-swarming phenotype. Further, 216 of the 294 mutants displayed no significant defects in swimming motility; therefore, the 216 genes were considered to be specifically associated with the swarming phenotype. The swarming-associated genes were classified into various functional categories, indicating that swarming is a specialized form of motility that requires a wide variety of cellular activities. These genes include genes for tricarboxylic acid cycle and glucose metabolism, iron acquisition, chaperones and protein-folding catalysts, signal transduction, and biosynthesis of cell surface components, such as lipopolysaccharide, the enterobacterial common antigen, and type 1 fimbriae. Lipopolysaccharide and the enterobacterial common antigen may be important surface-acting components that contribute to the reduction of surface tension, thereby facilitating the swarm migration in the E. coli K-12 strain.",
author = "Tetsuyoshi Inoue and Ryuji Shingaki and Shotaro Hirose and Kaori Waki and Hirotada Mori and Kazuhiro Fukui",
year = "2007",
month = "2",
doi = "10.1128/JB.01294-06",
language = "English",
volume = "189",
pages = "950--957",
journal = "Journal of Bacteriology",
issn = "0021-9193",
publisher = "American Society for Microbiology",
number = "3",

}

TY - JOUR

T1 - Genome-wide screening of genes required for swarming motility in Escherichia coli K-12

AU - Inoue, Tetsuyoshi

AU - Shingaki, Ryuji

AU - Hirose, Shotaro

AU - Waki, Kaori

AU - Mori, Hirotada

AU - Fukui, Kazuhiro

PY - 2007/2

Y1 - 2007/2

N2 - Escherichia coli K-12 has the ability to migrate on semisolid media by means of swarming motility. A systematic and comprehensive collection of gene-disrupted E. coli K-12 mutants (the Keio collection) was used to identify the genes involved in the swarming motility of this bacterium. Of the 3,985 nonessential gene mutants, 294 were found to exhibit a strongly repressed-swarming phenotype. Further, 216 of the 294 mutants displayed no significant defects in swimming motility; therefore, the 216 genes were considered to be specifically associated with the swarming phenotype. The swarming-associated genes were classified into various functional categories, indicating that swarming is a specialized form of motility that requires a wide variety of cellular activities. These genes include genes for tricarboxylic acid cycle and glucose metabolism, iron acquisition, chaperones and protein-folding catalysts, signal transduction, and biosynthesis of cell surface components, such as lipopolysaccharide, the enterobacterial common antigen, and type 1 fimbriae. Lipopolysaccharide and the enterobacterial common antigen may be important surface-acting components that contribute to the reduction of surface tension, thereby facilitating the swarm migration in the E. coli K-12 strain.

AB - Escherichia coli K-12 has the ability to migrate on semisolid media by means of swarming motility. A systematic and comprehensive collection of gene-disrupted E. coli K-12 mutants (the Keio collection) was used to identify the genes involved in the swarming motility of this bacterium. Of the 3,985 nonessential gene mutants, 294 were found to exhibit a strongly repressed-swarming phenotype. Further, 216 of the 294 mutants displayed no significant defects in swimming motility; therefore, the 216 genes were considered to be specifically associated with the swarming phenotype. The swarming-associated genes were classified into various functional categories, indicating that swarming is a specialized form of motility that requires a wide variety of cellular activities. These genes include genes for tricarboxylic acid cycle and glucose metabolism, iron acquisition, chaperones and protein-folding catalysts, signal transduction, and biosynthesis of cell surface components, such as lipopolysaccharide, the enterobacterial common antigen, and type 1 fimbriae. Lipopolysaccharide and the enterobacterial common antigen may be important surface-acting components that contribute to the reduction of surface tension, thereby facilitating the swarm migration in the E. coli K-12 strain.

UR - http://www.scopus.com/inward/record.url?scp=33846636385&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33846636385&partnerID=8YFLogxK

U2 - 10.1128/JB.01294-06

DO - 10.1128/JB.01294-06

M3 - Article

C2 - 17122336

AN - SCOPUS:33846636385

VL - 189

SP - 950

EP - 957

JO - Journal of Bacteriology

JF - Journal of Bacteriology

SN - 0021-9193

IS - 3

ER -