Genome-wide screening of genes required for swarming motility in Escherichia coli K-12

Tetsuyoshi Inoue, Ryuji Shingaki, Shotaro Hirose, Kaori Waki, Hirotada Mori, Kazuhiro Fukui

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Abstract

Escherichia coli K-12 has the ability to migrate on semisolid media by means of swarming motility. A systematic and comprehensive collection of gene-disrupted E. coli K-12 mutants (the Keio collection) was used to identify the genes involved in the swarming motility of this bacterium. Of the 3,985 nonessential gene mutants, 294 were found to exhibit a strongly repressed-swarming phenotype. Further, 216 of the 294 mutants displayed no significant defects in swimming motility; therefore, the 216 genes were considered to be specifically associated with the swarming phenotype. The swarming-associated genes were classified into various functional categories, indicating that swarming is a specialized form of motility that requires a wide variety of cellular activities. These genes include genes for tricarboxylic acid cycle and glucose metabolism, iron acquisition, chaperones and protein-folding catalysts, signal transduction, and biosynthesis of cell surface components, such as lipopolysaccharide, the enterobacterial common antigen, and type 1 fimbriae. Lipopolysaccharide and the enterobacterial common antigen may be important surface-acting components that contribute to the reduction of surface tension, thereby facilitating the swarm migration in the E. coli K-12 strain.

Original languageEnglish
Pages (from-to)950-957
Number of pages8
JournalJournal of Bacteriology
Volume189
Issue number3
DOIs
Publication statusPublished - Feb 1 2007

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ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

Inoue, T., Shingaki, R., Hirose, S., Waki, K., Mori, H., & Fukui, K. (2007). Genome-wide screening of genes required for swarming motility in Escherichia coli K-12. Journal of Bacteriology, 189(3), 950-957. https://doi.org/10.1128/JB.01294-06