Genome-wide identification and expression analysis of the CaNAC family members in chickpea during development, dehydration and ABA treatments

Chien Van Ha, Maryam Nasr Esfahani, Yasuko Watanabe, Uyen Thi Tran, Saad Sulieman, Keiichi Mochida, Dong Van Nguyen, Lam Son Phan Tran

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The plant-specific NAC transcription factors (TFs) play important roles in regulation of diverse biological processes, including development, growth, cell division and responses to environmental stimuli. In this study, we identified the members of the NAC TF family of chickpea (Cicer arietinum) and assess their expression profiles during plant development and under dehydration and abscisic acid (ABA) treatments in a systematic manner. Seventy-one CaNAC genes were detected from the chickpea genome, including 8 membrane-bound members of which many might be involved in dehydration responses as judged from published literature. Phylogenetic analysis of the chickpea and well-known stress-related Arabidopsis and rice NACs enabled us to predict several putative stress-related CaNACs. By exploring available transcriptome data, we provided a comprehensive expression atlas of CaNACs in various tissues at different developmental stages. With the highest interest in dehydration responses, we examined the expression of the predicted stress-related and membrane-bound CaNACs in roots and leaves of chickpea seedlings, subjected to well-watered (control), dehydration and ABA treatments, using real-time quantitative PCR (RT-qPCR). Nine-teen of the 23 CaNACs examined were found to be dehydration-responsive in chickpea roots and/ or leaves in either ABA-dependent or -independent pathway. Our results have provided a solid foundation for selection of promising tissue-specific and/or dehydration-responsive CaNAC candidates for detailed in planta functional analyses, leading to development of transgenic chickpea varieties with improved productivity under drought.

Original languageEnglish
Article numbere114107
JournalPLoS One
Volume9
Issue number12
DOIs
Publication statusPublished - Dec 5 2014
Externally publishedYes

Fingerprint

Cicer
Abscisic Acid
desiccation (plant physiology)
acid treatment
Dehydration
abscisic acid
Genes
Genome
genome
Transcription Factors
Therapeutics
transcription factors
Tissue
Membranes
Drought
Biological Phenomena
Cell growth
Cicer arietinum
Plantae
Plant Development

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Genome-wide identification and expression analysis of the CaNAC family members in chickpea during development, dehydration and ABA treatments. / Ha, Chien Van; Esfahani, Maryam Nasr; Watanabe, Yasuko; Tran, Uyen Thi; Sulieman, Saad; Mochida, Keiichi; Nguyen, Dong Van; Tran, Lam Son Phan.

In: PLoS One, Vol. 9, No. 12, e114107, 05.12.2014.

Research output: Contribution to journalArticle

Ha, Chien Van ; Esfahani, Maryam Nasr ; Watanabe, Yasuko ; Tran, Uyen Thi ; Sulieman, Saad ; Mochida, Keiichi ; Nguyen, Dong Van ; Tran, Lam Son Phan. / Genome-wide identification and expression analysis of the CaNAC family members in chickpea during development, dehydration and ABA treatments. In: PLoS One. 2014 ; Vol. 9, No. 12.
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