Genome-Wide Association Studies (GWAS) for Yield and Weevil Resistance in Sweet potato (Ipomoea batatas (L.) Lam)

Yoshihiro Okada, Yuki Monden, Kanoko Nokihara, Kenta Shirasawa, Sachiko Isobe, Makoto Tahara

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Key message: We apply the GWAS to sweet potato genome, and identified the SNPs associated with yield and weevil resistance. Abstract: The sweet potato (Ipomoea batatas (L.) Lam) is a highly heterozygous, outcrossing, polyploid species, which presents challenges for genetic analysis. Therefore, we considered that genome-wide association studies (GWAS) may be applied to the study of the sweet potato genome. The yield of two sweet potato varieties [Purple Sweet Lord (PSL) and 90IDN-47] was assessed at two locations (Kumamoto and Okinawa prefectures) in Japan in 2013 and the yield scores were used for GWAS. The results showed that there were several single nucleotide polymorphisms (SNP) above the significance thresholds in PSL; two peaks were detected in Kumamoto and Okinawa on the Ib03-3 and Ib01-4 linkage groups of PSL, respectively. As for 90IDN-47, one relatively high peak was detected in Kumamoto on the Ib13-8 linkage group. Interestingly, although high peaks above significance thresholds were detected in Kumamoto and Okinawa in PSL, the peaks were located in different linkage groups. This result suggests that the genetic regions controlling yield may change in response to environmental conditions. Additionally, we investigated the degree of weevil damage to the plants, which is the greatest problem in sweet potato cultivation in Okinawa. In this experiment, no SNPs were identified above the significance thresholds. However, one relatively high peak was found in the 90IDN-47 genotype, which showed resistance to weevils. On the other hand, one relatively high peak was also detected in the PSL genotype, which showed susceptibility to weevils. These results suggest that two regions could affect weevil resistance and may contain the gene(s) controlling weevil resistance.

Original languageEnglish
JournalPlant Cell Reports
DOIs
Publication statusPublished - Jan 1 2019

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Ipomoea batatas
sweet potatoes
Curculionidae
Ryukyu Archipelago
linkage groups
genome
genotype
outcrossing
polyploidy
single nucleotide polymorphism
genetic techniques and protocols
genome-wide association study
Japan
environmental factors
genes

Keywords

  • Convolvulaceae
  • Cylas formicarius
  • Euscepes postfasciatus
  • Genome analysis

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

Cite this

Genome-Wide Association Studies (GWAS) for Yield and Weevil Resistance in Sweet potato (Ipomoea batatas (L.) Lam). / Okada, Yoshihiro; Monden, Yuki; Nokihara, Kanoko; Shirasawa, Kenta; Isobe, Sachiko; Tahara, Makoto.

In: Plant Cell Reports, 01.01.2019.

Research output: Contribution to journalArticle

Okada, Yoshihiro ; Monden, Yuki ; Nokihara, Kanoko ; Shirasawa, Kenta ; Isobe, Sachiko ; Tahara, Makoto. / Genome-Wide Association Studies (GWAS) for Yield and Weevil Resistance in Sweet potato (Ipomoea batatas (L.) Lam). In: Plant Cell Reports. 2019.
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