Genome-wide association mapping of cadmium accumulation in different organs of barley

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The threshold value of cadmium (Cd) concentration in grains of barley (Hordeum vulgare) is the lowest among cereal crops; however, it is poorly understood how Cd accumulation in barley grain is genetically controlled. We investigated genotypic variation in Cd accumulation of different organs in 100 accessions from a subset of the barley core collection using both hydroponic and Cd-contaminated soil culture. We also performed a genome-wide association (GWA) mapping for Cd accumulation in different organs. A large genotypic variation in the Cd concentration was found in all organs. There was a good correlation between shoot Cd of solution and soil culture, the shoot Cd and grain Cd, but no correlation between the root Cd and grain Cd. GWA mapping detected 9 quantitative trait loci (QTL) for root Cd, 21 for shoot Cd, 14 for root-to-shoot translocation and 15 for grain Cd. A common QTL for the shoot Cd and root-to-shoot translocation was found at 132.6 cM on chromosome 5H. Two major QTL for grain Cd were identified on chromosome 2H and chromosome 5H. The genetic variation in Cd accumulation and major QTL detected provide useful information helpful for cloning candidate genes for Cd accumulation and breeding low-Cd barley cultivars in future.

Original languageEnglish
Pages (from-to)817-829
Number of pages13
JournalNew Phytologist
Volume208
Issue number3
DOIs
Publication statusPublished - Nov 1 2015

Fingerprint

Hordeum
Cadmium
chromosome mapping
cadmium
barley
Genome
genome
Quantitative Trait Loci
shoots
quantitative trait loci
Chromosomes
chromosomes
Soil
Hydroponics
grain crops

Keywords

  • Barley
  • Cadmium (Cd)
  • Core collection
  • Genome-wide association (GWA) mapping
  • Genotypic difference
  • Quantitative trait loci (QTL)
  • Straw

ASJC Scopus subject areas

  • Plant Science
  • Physiology

Cite this

Genome-wide association mapping of cadmium accumulation in different organs of barley. / Wu, Dezhi; Sato, Kazuhiro; Ma, Jian Feng.

In: New Phytologist, Vol. 208, No. 3, 01.11.2015, p. 817-829.

Research output: Contribution to journalArticle

@article{5a289111f60740debf4002bc4560732e,
title = "Genome-wide association mapping of cadmium accumulation in different organs of barley",
abstract = "The threshold value of cadmium (Cd) concentration in grains of barley (Hordeum vulgare) is the lowest among cereal crops; however, it is poorly understood how Cd accumulation in barley grain is genetically controlled. We investigated genotypic variation in Cd accumulation of different organs in 100 accessions from a subset of the barley core collection using both hydroponic and Cd-contaminated soil culture. We also performed a genome-wide association (GWA) mapping for Cd accumulation in different organs. A large genotypic variation in the Cd concentration was found in all organs. There was a good correlation between shoot Cd of solution and soil culture, the shoot Cd and grain Cd, but no correlation between the root Cd and grain Cd. GWA mapping detected 9 quantitative trait loci (QTL) for root Cd, 21 for shoot Cd, 14 for root-to-shoot translocation and 15 for grain Cd. A common QTL for the shoot Cd and root-to-shoot translocation was found at 132.6 cM on chromosome 5H. Two major QTL for grain Cd were identified on chromosome 2H and chromosome 5H. The genetic variation in Cd accumulation and major QTL detected provide useful information helpful for cloning candidate genes for Cd accumulation and breeding low-Cd barley cultivars in future.",
keywords = "Barley, Cadmium (Cd), Core collection, Genome-wide association (GWA) mapping, Genotypic difference, Quantitative trait loci (QTL), Straw",
author = "Dezhi Wu and Kazuhiro Sato and Ma, {Jian Feng}",
year = "2015",
month = "11",
day = "1",
doi = "10.1111/nph.13512",
language = "English",
volume = "208",
pages = "817--829",
journal = "New Phytologist",
issn = "0028-646X",
publisher = "Wiley-Blackwell",
number = "3",

}

TY - JOUR

T1 - Genome-wide association mapping of cadmium accumulation in different organs of barley

AU - Wu, Dezhi

AU - Sato, Kazuhiro

AU - Ma, Jian Feng

PY - 2015/11/1

Y1 - 2015/11/1

N2 - The threshold value of cadmium (Cd) concentration in grains of barley (Hordeum vulgare) is the lowest among cereal crops; however, it is poorly understood how Cd accumulation in barley grain is genetically controlled. We investigated genotypic variation in Cd accumulation of different organs in 100 accessions from a subset of the barley core collection using both hydroponic and Cd-contaminated soil culture. We also performed a genome-wide association (GWA) mapping for Cd accumulation in different organs. A large genotypic variation in the Cd concentration was found in all organs. There was a good correlation between shoot Cd of solution and soil culture, the shoot Cd and grain Cd, but no correlation between the root Cd and grain Cd. GWA mapping detected 9 quantitative trait loci (QTL) for root Cd, 21 for shoot Cd, 14 for root-to-shoot translocation and 15 for grain Cd. A common QTL for the shoot Cd and root-to-shoot translocation was found at 132.6 cM on chromosome 5H. Two major QTL for grain Cd were identified on chromosome 2H and chromosome 5H. The genetic variation in Cd accumulation and major QTL detected provide useful information helpful for cloning candidate genes for Cd accumulation and breeding low-Cd barley cultivars in future.

AB - The threshold value of cadmium (Cd) concentration in grains of barley (Hordeum vulgare) is the lowest among cereal crops; however, it is poorly understood how Cd accumulation in barley grain is genetically controlled. We investigated genotypic variation in Cd accumulation of different organs in 100 accessions from a subset of the barley core collection using both hydroponic and Cd-contaminated soil culture. We also performed a genome-wide association (GWA) mapping for Cd accumulation in different organs. A large genotypic variation in the Cd concentration was found in all organs. There was a good correlation between shoot Cd of solution and soil culture, the shoot Cd and grain Cd, but no correlation between the root Cd and grain Cd. GWA mapping detected 9 quantitative trait loci (QTL) for root Cd, 21 for shoot Cd, 14 for root-to-shoot translocation and 15 for grain Cd. A common QTL for the shoot Cd and root-to-shoot translocation was found at 132.6 cM on chromosome 5H. Two major QTL for grain Cd were identified on chromosome 2H and chromosome 5H. The genetic variation in Cd accumulation and major QTL detected provide useful information helpful for cloning candidate genes for Cd accumulation and breeding low-Cd barley cultivars in future.

KW - Barley

KW - Cadmium (Cd)

KW - Core collection

KW - Genome-wide association (GWA) mapping

KW - Genotypic difference

KW - Quantitative trait loci (QTL)

KW - Straw

UR - http://www.scopus.com/inward/record.url?scp=84945465806&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84945465806&partnerID=8YFLogxK

U2 - 10.1111/nph.13512

DO - 10.1111/nph.13512

M3 - Article

C2 - 26061418

AN - SCOPUS:84945465806

VL - 208

SP - 817

EP - 829

JO - New Phytologist

JF - New Phytologist

SN - 0028-646X

IS - 3

ER -