Genome sequence analysis of new plum pox virus isolates from Japan

Tomoaki Mori, Chiaki Warner, Serika Ohno, Koichi Mori, Takamasa Tobimatsu, Takashi Sera

Research output: Contribution to journalArticlepeer-review

Abstract

Objective: To find mutations that may have recently occurred in Plum pox virus (PPV), we collected six PPV-infected plum/peach trees from the western part of Japan and one from the eastern part. After sequencing the full-length PPV genomic RNAs, we compared the amino acid sequences with representative isolates of each PPV strain. Results: All new isolates were found to belong to the PPV-D strain: the six isolates collected from western Japan were identified as the West-Japan strain while the one collected from eastern Japan as the East-Japan strain. Amino acid sequence analysis of these seven isolates suggested that the 1407th and 1529th amino acid residues are characteristic of the West-Japan and the East-Japan strains, respectively. Comparing them with the corresponding amino acid residues of the 47 non-Japanese PPV-D isolates revealed that these amino acid residues are undoubtedly unique. A further examination of the relevant amino acid residues of the other 210 PPV-D isolates collected in Japan generated a new hypothesis regarding the invasion route from overseas and the subsequent diffusion route within Japan: a PPV-D strain might have invaded the western part of Japan from overseas and spread throughout Japan.

Original languageEnglish
Article number266
JournalBMC Research Notes
Volume14
Issue number1
DOIs
Publication statusPublished - Dec 2021

Keywords

  • Complete genome sequence
  • Genetic variation
  • Phylogenetic analysis
  • Plum pox virus
  • Sequence alignment analysis

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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