Genome rearrangement of a mycovirus Rosellinia necatrix megabirnavirus 1 affecting its ability to attenuate virulence of the host fungus

Satoko Kanematsu, Takeo Shimizu, Lakha Salaipeth, Hajime Yaegashi, Atsuko Sasaki, Tsutae Ito, Nobuhiro Suzuki

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Rosellinia necatrix megabirnavirus 1 (RnMBV1) is a bi-segmented double-stranded RNA mycovirus that reduces the virulence of the fungal plant pathogen R. necatrix. We isolated strains of RnMBV1 with genome rearrangements (RnMBV1-RS1) that retained dsRNA1, encoding capsid protein (ORF1) and RNA-dependent RNA polymerase (ORF2), and had a newly emerged segment named dsRNAS1, but with loss of dsRNA2, which contains two ORFs of unknown function. Analyses of two variants of dsRNAS1 revealed that they both originated from dsRNA1 by deletion of ORF1 and partial tandem duplication of ORF2, retaining a much shorter 5' untranslated region (UTR). R. necatrix transfected with RnMBV-RS1 virions showed maintenance of virulence on host plants compared with infection with RnMBV1. This suggests that dsRNAS1 is able to be transcribed and packaged, as well as suggesting that dsRNA2, while dispensable for virus replication, is required to reduce the virulence of R. necatrix.

Original languageEnglish
Pages (from-to)308-315
Number of pages8
JournalVirology
Volume450-451
DOIs
Publication statusPublished - Feb 1 2014

Keywords

  • Fungal virus
  • Genomic rearrangement
  • Virulence

ASJC Scopus subject areas

  • Virology

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