Genome-Edited Triple-Recessive Mutation Alters Seed Dormancy in Wheat

Fumitaka Abe, Emdadul Haque, Hiroshi Hisano, Tsuyoshi Tanaka, Yoko Kamiya, Masafumi Mikami, Kanako Kawaura, Masaki Endo, Kazumitsu Onishi, Takeshi Hayashi, Kazuhiro Sato

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Common wheat has three sets of sub-genomes, making mutations difficult to observe, especially for traits controlled by recessive genes. Here, we produced hexaploid wheat lines with loss of function of homeoalleles of Qsd1, which controls seed dormancy in barley, by Agrobacterium-mediated CRISPR/Cas9. Of the eight transformed wheat events produced, three independent events carrying multiple mutations in wheat Qsd1 homeoalleles were obtained. Notably, one line had mutations in every homeoallele. We crossed this plant with wild-type cultivar Fielder to generate a transgene-free triple-recessive mutant, as revealed by Mendelian segregation. The mutant showed a significantly longer seed dormancy period than wild-type, which may result in reduced pre-harvest sprouting of grains on spikes. PCR, southern blotting, and whole-genome shotgun sequencing revealed that this segregant lacked transgenes in its genomic sequence. This technique serves as a model for trait improvement in wheat, particularly for genetically recessive traits, based on locus information from diploid barley. Using Agrobacterium-delivered CRISPR/Cas9, Abe et al. developed a loss-of-function triple mutation of Qsd1, which controls seed dormancy in barley, resulting in longer seed dormancy in wheat. This serves as a model technique for the improvement of wheat traits, particularly genetically recessive traits, based on locus information for diploid barley.

Original languageEnglish
Pages (from-to)1362-1369.e4
JournalCell Reports
Volume28
Issue number5
DOIs
Publication statusPublished - Jul 30 2019

Fingerprint

Plant Dormancy
Triticum
Seed
Clustered Regularly Interspaced Short Palindromic Repeats
Genes
Genome
Mutation
Hordeum
Agrobacterium
Diploidy
Transgenes
Recessive Genes
Firearms
Southern Blotting
Polymerase Chain Reaction

Keywords

  • CRISPR/Cas9
  • multiple mutation
  • Qsd1
  • seed dormancy
  • wheat

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Genome-Edited Triple-Recessive Mutation Alters Seed Dormancy in Wheat. / Abe, Fumitaka; Haque, Emdadul; Hisano, Hiroshi; Tanaka, Tsuyoshi; Kamiya, Yoko; Mikami, Masafumi; Kawaura, Kanako; Endo, Masaki; Onishi, Kazumitsu; Hayashi, Takeshi; Sato, Kazuhiro.

In: Cell Reports, Vol. 28, No. 5, 30.07.2019, p. 1362-1369.e4.

Research output: Contribution to journalArticle

Abe, F, Haque, E, Hisano, H, Tanaka, T, Kamiya, Y, Mikami, M, Kawaura, K, Endo, M, Onishi, K, Hayashi, T & Sato, K 2019, 'Genome-Edited Triple-Recessive Mutation Alters Seed Dormancy in Wheat', Cell Reports, vol. 28, no. 5, pp. 1362-1369.e4. https://doi.org/10.1016/j.celrep.2019.06.090
Abe, Fumitaka ; Haque, Emdadul ; Hisano, Hiroshi ; Tanaka, Tsuyoshi ; Kamiya, Yoko ; Mikami, Masafumi ; Kawaura, Kanako ; Endo, Masaki ; Onishi, Kazumitsu ; Hayashi, Takeshi ; Sato, Kazuhiro. / Genome-Edited Triple-Recessive Mutation Alters Seed Dormancy in Wheat. In: Cell Reports. 2019 ; Vol. 28, No. 5. pp. 1362-1369.e4.
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