Genome aberrations observed by restriction landmark genome scanning analysis of chromosomal DNA in various types of primary hepatocellular carcinoma

Tadashi Hanafusa, Yasuhiro Yumoto, Tetsushige Mimura, Toshiyuki Shinji, Hajime Hada, Norio Koide, Takao Tsuji

Research output: Contribution to journalArticle

3 Citations (Scopus)


Background/Aims: The genes responsible for hepatocellular carcinoma have not been identified. To identify the relevant genes of hepatocellular carcinoma, detailed and comprehensive information of genomic aberrations must be obtained. To reveal the chromosomal aberrations in hepatocellular carcinoma, we carried out a restriction landmark genome scanning analysis of various types of hepatocellular carcinoma. Methodology: Samples of various types of hepatocellular carcinoma, including two with multinodular-hepatocellular carcinomas, one hepatocellular carcinoma showing nodules in a nodule pattern, one hepatocellular carcinoma metastasized to different tissues, three small (5.0cm) hepatocellular carcinomas were examined by the restriction landmark genome scanning method with corresponding non-hepatocellular carcinoma tissues. Restriction enzyme Not I was used as a landmark enzyme, Eco RV was used as a fragmentation enzyme, and Hin fI was used as a digestion enzyme in the gel for two-dimensional electorophoresis. Results: We observed spot aberrations with different origins. Frequently observed spot aberrations originated from the change in the methylation status of repetitive sequences. No clear correlation between the pathological grade and the number or type of spot aberrations was observed. Conclusions: We demonstrated that major aberrations of restriction landmark genome scanning spots originated from the change of methylation status in hepatocellular carcinoma.

Original languageEnglish
Pages (from-to)482-486
Number of pages5
Issue number44
Publication statusPublished - 2002



  • Genome methylation
  • HCC
  • Repetitive sequence
  • RLGS analysis

ASJC Scopus subject areas

  • Gastroenterology

Cite this