Genetically determined differences in sodium current characteristics modulate conduction disease severity in mice with cardiac sodium channelopathy

Carol Ann Remme, Brendon P. Scicluna, Arie O. Verkerk, Ahmad S. Amin, Sandra Van Brunschot, Leander Beekman, Vera H.M. Deneer, Catherine Chevalier, Fumitaka Oyama, Haruko Miyazaki, Nobuyuki Nukina, Ronald Wilders, Denis Escande, Rémi Houlgatte, Arthur A.M. Wilde, Hanno L. Tan, Marieke W. Veldkamp, Jacques M.T. De Bakker, Connie R. Bezzina

Research output: Contribution to journalArticlepeer-review

66 Citations (Scopus)

Abstract

Conduction slowing of the electric impulse that drives the heartbeat may evoke lethal cardiac arrhythmias. Mutations in SCN5A, which encodes the pore-forming cardiac sodium channel α subunit, are associated with familial arrhythmia syndromes based on conduction slowing. However, disease severity among mutation carriers is highly variable. We hypothesized that genetic modifiers underlie the variability in conduction slowing and disease severity. With the aim of identifying such modifiers, we studied the Scn5a mutation in 2 distinct mouse strains, FVB/N and 129P2. In 129P2 mice, the mutation resulted in more severe conduction slowing particularly in the right ventricle (RV) compared to FVB/N. Pan-genomic mRNA expression profiling in the 2 mouse strains uncovered a drastic reduction in mRNA encoding the sodium channel auxiliary subunit β4 (Scn4b) in 129P2 mice compared to FVB/N. This corresponded to low to undetectable β4 protein levels in 129P2 ventricular tissue, whereas abundant β4 protein was detected in FVB/N. Sodium current measurements in isolated myocytes from the 2 mouse strains indicated that sodium channel activation in myocytes from 129P2 mice occurred at more positive potentials compared to FVB/N. Using computer simulations, this difference in activation kinetics was predicted to explain the observed differences in conduction disease severity between the 2 strains. In conclusion, genetically determined differences in sodium current characteristics on the myocyte level modulate disease severity in cardiac sodium channelopathies. In particular, the sodium channel subunit β4 (SCN4B) may constitute a potential genetic modifier of conduction and cardiac sodium channel disease.

Original languageEnglish
Pages (from-to)1283-1292
Number of pages10
JournalCirculation research
Volume104
Issue number11
DOIs
Publication statusPublished - Jun 5 2009
Externally publishedYes

Keywords

  • Conduction
  • Gene expression
  • Genetics
  • Mouse mutants
  • Sodium channels

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

Fingerprint

Dive into the research topics of 'Genetically determined differences in sodium current characteristics modulate conduction disease severity in mice with cardiac sodium channelopathy'. Together they form a unique fingerprint.

Cite this