TY - JOUR
T1 - Genetic variation of high-molecular-weight glutenin subunit composition in Asian wheat
AU - Terasawa, Yohei
AU - Takata, Kanenori
AU - Hirano, Hisashi
AU - Kato, Kenji
AU - Kawahara, Taihachi
AU - Sasakuma, Tetsuo
AU - Sasanuma, Tsuneo
N1 - Funding Information:
Acknowledgments This work was supported by the National BioResources Project (NBRP) KOMUGI and a Grant-in-Aid for Scientific Research (A) (10201047). We express our sincere gratitude to Drs. Tatsuya Ikeda, Hisashi Tsujimoto, Motoko Takaoka, and Toshinori Abe for their technical support and critical discussion. Finally, we thank all the members of the Laboratory of Evolutionary Genetics of the Kihara Institute for Biological Research for their kind help and continuous encouragement throughout this study.
PY - 2011/2
Y1 - 2011/2
N2 - To clarify the genetic properties of the HMW glutenin subunit composition of Asian endemic wheats, SDS-PAGE analysis was conducted using 1,139 bread wheat accessions that were originally collected in Asia. The samples were divided into six regional groups, Western Asia, Caucasia, Central Asia, Afghanistan, Southern Asia, and Eastern Asia. The genotype Glu-A1c, Glu-B1b, and Glu-D1a encoding subunits null, 7+8, and 2+12 had an overall frequency of 55.2%. Thus, we conclude that it is the typical genotype of the HMW glutenin subunits that characterize Asian endemic wheat. The frequency of the typical Asian genotype was relatively high in the central belt of Asia (Western Asia, Afghanistan, and Eastern Asia) and low in the marginal regions (Caucasia, Central Asia, and Southern Asia). In Southern Asia, the frequency of Glu-B1i, which encodes subunit 17+18, was the highest at the Glu-B1 locus. In Caucasia and Central Asia, the frequency of Glu-D1d, which encodes subunit 5+10 (which is considered to be the most useful for making bread), was high. The level of genetic variation, as estimated using the frequencies of the various alleles, was relatively low in the central belt of Asia and high in the marginal regions. Among the three Glu-1 loci, the highest number of alleles was detected at the Glu-D1 locus. This result was caused by the presence of rare Asian specific alleles at the Glu-D1 locus, in which a newly found allele, Glu-D1bs, encoding subunit 2.1+12 was included.
AB - To clarify the genetic properties of the HMW glutenin subunit composition of Asian endemic wheats, SDS-PAGE analysis was conducted using 1,139 bread wheat accessions that were originally collected in Asia. The samples were divided into six regional groups, Western Asia, Caucasia, Central Asia, Afghanistan, Southern Asia, and Eastern Asia. The genotype Glu-A1c, Glu-B1b, and Glu-D1a encoding subunits null, 7+8, and 2+12 had an overall frequency of 55.2%. Thus, we conclude that it is the typical genotype of the HMW glutenin subunits that characterize Asian endemic wheat. The frequency of the typical Asian genotype was relatively high in the central belt of Asia (Western Asia, Afghanistan, and Eastern Asia) and low in the marginal regions (Caucasia, Central Asia, and Southern Asia). In Southern Asia, the frequency of Glu-B1i, which encodes subunit 17+18, was the highest at the Glu-B1 locus. In Caucasia and Central Asia, the frequency of Glu-D1d, which encodes subunit 5+10 (which is considered to be the most useful for making bread), was high. The level of genetic variation, as estimated using the frequencies of the various alleles, was relatively low in the central belt of Asia and high in the marginal regions. Among the three Glu-1 loci, the highest number of alleles was detected at the Glu-D1 locus. This result was caused by the presence of rare Asian specific alleles at the Glu-D1 locus, in which a newly found allele, Glu-D1bs, encoding subunit 2.1+12 was included.
KW - Asian endemic wheat
KW - Genetic variation
KW - Glu-1 loci
KW - HMW-glutenin subunit
KW - Triticum aestivum
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U2 - 10.1007/s10722-010-9573-5
DO - 10.1007/s10722-010-9573-5
M3 - Article
AN - SCOPUS:79951550182
VL - 58
SP - 283
EP - 289
JO - Genetic Resources and Crop Evolution
JF - Genetic Resources and Crop Evolution
SN - 0925-9864
IS - 2
ER -