Genetic Variation for Seed Metabolite Levels in Brachypodium distachyon

Yoshihiko Onda, Komaki Inoue, Yuji Sawada, Minami Shimizu, Kotaro Takahagi, Yukiko Uehara-Yamaguchi, Masami Y. Hirai, David F. Garvin, Keiichi Mochida

Research output: Contribution to journalArticle

Abstract

Metabolite composition and concentrations in seed grains are important traits of cereals. To identify the variation in the seed metabolotypes of a model grass, namely Brachypodium distachyon, we applied a widely targeted metabolome analysis to forty inbred lines of B. distachyon and examined the accumulation patterns of 183 compounds in the seeds. By comparing the metabolotypes with the population structure of these lines, we found signature metabolites that represent different accumulation patterns for each of the three B. distachyon subpopulations. Moreover, we found that thirty-seven metabolites exhibited significant differences in their accumulation between the lines Bd21 and Bd3-1. Using a recombinant inbred line (RIL) population from a cross between Bd3-1 and Bd21, we identified the quantitative trait loci (QTLs) linked with this variation in the accumulation of thirteen metabolites. Our metabolite QTL analysis illustrated that different genetic factors may presumably regulate the accumulation of 4-pyridoxate and pyridoxamine in vitamin B6 metabolism. Moreover, we found two QTLs on chromosomes 1 and 4 that affect the accumulation of an anthocyanin, chrysanthemin. These QTLs genetically interacted to regulate the accumulation of this compound. This study demonstrates the potential for metabolite QTL mapping in B. distachyon and provides new insights into the genetic dissection of metabolomic traits in temperate grasses.

Original languageEnglish
JournalInternational journal of molecular sciences
Volume20
Issue number9
DOIs
Publication statusPublished - May 11 2019

Fingerprint

Brachypodium
metabolites
Quantitative Trait Loci
Metabolites
Seed
seeds
Seeds
loci
Poaceae
grasses
Pyridoxamine
Chromosomes, Human, Pair 4
Vitamin B 6
Metabolomics
Metabolome
Anthocyanins
Chromosomes, Human, Pair 1
Dissection
Vitamins
Population

Keywords

  • Brachypodium distachyon
  • chrysanthemin
  • metabolome
  • QTL analysis
  • recombinant inbred line
  • vitamin B6

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

Cite this

Genetic Variation for Seed Metabolite Levels in Brachypodium distachyon. / Onda, Yoshihiko; Inoue, Komaki; Sawada, Yuji; Shimizu, Minami; Takahagi, Kotaro; Uehara-Yamaguchi, Yukiko; Hirai, Masami Y.; Garvin, David F.; Mochida, Keiichi.

In: International journal of molecular sciences, Vol. 20, No. 9, 11.05.2019.

Research output: Contribution to journalArticle

Onda, Y, Inoue, K, Sawada, Y, Shimizu, M, Takahagi, K, Uehara-Yamaguchi, Y, Hirai, MY, Garvin, DF & Mochida, K 2019, 'Genetic Variation for Seed Metabolite Levels in Brachypodium distachyon', International journal of molecular sciences, vol. 20, no. 9. https://doi.org/10.3390/ijms20092348
Onda, Yoshihiko ; Inoue, Komaki ; Sawada, Yuji ; Shimizu, Minami ; Takahagi, Kotaro ; Uehara-Yamaguchi, Yukiko ; Hirai, Masami Y. ; Garvin, David F. ; Mochida, Keiichi. / Genetic Variation for Seed Metabolite Levels in Brachypodium distachyon. In: International journal of molecular sciences. 2019 ; Vol. 20, No. 9.
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