Genetic engineering of the unsaturation of fatty acids in membrane lipids alters the tolerance of Synechocystis to salt stress

Suleyman Allakhverdiev, Yoshitaka Nishiyama, Iwane Suzuki, Yasushi Tasaka, Norio Murata

Research output: Contribution to journalArticle

144 Citations (Scopus)

Abstract

The role of unsaturated fatty acids in membrane lipids in the tolerance of the photosynthetic machinery to salt stress was studied by comparing the desA-/desDmutant of Synechocystis sp. PCC 6803, which contained monounsaturated fatty acids, with the wild-type strain, which contained a full complement of polyunsaturated fatty acids. In darkness, the loss of oxygen-evolving photosystem II activity in the presence of 0.5 M NaCl or 0.5 M LiCl was much more rapid in desA-/desD- cells than in wild-type cells. Oxygen-evolving activity that had been lost during incubation with 0.5 M NaCl in darkness returned when cells were transferred to conditions that allowed photosynthesis or respiration. Recovery was much greater in wild-type than in desA-/desD- cells, and it was prevented by lincomycin. Thus, the unsaturation of fatty acids is important in the tolerance of the photosynthetic machinery to salt stress. It appears also that the activity and synthesis of the Na+/H+ antiporter system might be suppressed under high-salt conditions and that this effect can be reversed, in part, by the unsaturation of fatty acids in membrane lipids.

Original languageEnglish
Pages (from-to)5862-5867
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume96
Issue number10
DOIs
Publication statusPublished - May 11 1999
Externally publishedYes

Fingerprint

Synechocystis
Genetic Engineering
Membrane Lipids
Fatty Acids
Salts
Darkness
Unsaturated Fatty Acids
Oxygen
Lincomycin
Monounsaturated Fatty Acids
Sodium-Hydrogen Antiporter
Photosystem II Protein Complex
Photosynthesis
Respiration

Keywords

  • desA
  • desD
  • H antiport
  • Na
  • Photosystem II
  • Salt tolerance

ASJC Scopus subject areas

  • General

Cite this

Genetic engineering of the unsaturation of fatty acids in membrane lipids alters the tolerance of Synechocystis to salt stress. / Allakhverdiev, Suleyman; Nishiyama, Yoshitaka; Suzuki, Iwane; Tasaka, Yasushi; Murata, Norio.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, No. 10, 11.05.1999, p. 5862-5867.

Research output: Contribution to journalArticle

@article{27dee9f3c8b946fa9a0990c5dcafdf74,
title = "Genetic engineering of the unsaturation of fatty acids in membrane lipids alters the tolerance of Synechocystis to salt stress",
abstract = "The role of unsaturated fatty acids in membrane lipids in the tolerance of the photosynthetic machinery to salt stress was studied by comparing the desA-/desDmutant of Synechocystis sp. PCC 6803, which contained monounsaturated fatty acids, with the wild-type strain, which contained a full complement of polyunsaturated fatty acids. In darkness, the loss of oxygen-evolving photosystem II activity in the presence of 0.5 M NaCl or 0.5 M LiCl was much more rapid in desA-/desD- cells than in wild-type cells. Oxygen-evolving activity that had been lost during incubation with 0.5 M NaCl in darkness returned when cells were transferred to conditions that allowed photosynthesis or respiration. Recovery was much greater in wild-type than in desA-/desD- cells, and it was prevented by lincomycin. Thus, the unsaturation of fatty acids is important in the tolerance of the photosynthetic machinery to salt stress. It appears also that the activity and synthesis of the Na+/H+ antiporter system might be suppressed under high-salt conditions and that this effect can be reversed, in part, by the unsaturation of fatty acids in membrane lipids.",
keywords = "desA, desD, H antiport, Na, Photosystem II, Salt tolerance",
author = "Suleyman Allakhverdiev and Yoshitaka Nishiyama and Iwane Suzuki and Yasushi Tasaka and Norio Murata",
year = "1999",
month = "5",
day = "11",
doi = "10.1073/pnas.96.10.5862",
language = "English",
volume = "96",
pages = "5862--5867",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "10",

}

TY - JOUR

T1 - Genetic engineering of the unsaturation of fatty acids in membrane lipids alters the tolerance of Synechocystis to salt stress

AU - Allakhverdiev, Suleyman

AU - Nishiyama, Yoshitaka

AU - Suzuki, Iwane

AU - Tasaka, Yasushi

AU - Murata, Norio

PY - 1999/5/11

Y1 - 1999/5/11

N2 - The role of unsaturated fatty acids in membrane lipids in the tolerance of the photosynthetic machinery to salt stress was studied by comparing the desA-/desDmutant of Synechocystis sp. PCC 6803, which contained monounsaturated fatty acids, with the wild-type strain, which contained a full complement of polyunsaturated fatty acids. In darkness, the loss of oxygen-evolving photosystem II activity in the presence of 0.5 M NaCl or 0.5 M LiCl was much more rapid in desA-/desD- cells than in wild-type cells. Oxygen-evolving activity that had been lost during incubation with 0.5 M NaCl in darkness returned when cells were transferred to conditions that allowed photosynthesis or respiration. Recovery was much greater in wild-type than in desA-/desD- cells, and it was prevented by lincomycin. Thus, the unsaturation of fatty acids is important in the tolerance of the photosynthetic machinery to salt stress. It appears also that the activity and synthesis of the Na+/H+ antiporter system might be suppressed under high-salt conditions and that this effect can be reversed, in part, by the unsaturation of fatty acids in membrane lipids.

AB - The role of unsaturated fatty acids in membrane lipids in the tolerance of the photosynthetic machinery to salt stress was studied by comparing the desA-/desDmutant of Synechocystis sp. PCC 6803, which contained monounsaturated fatty acids, with the wild-type strain, which contained a full complement of polyunsaturated fatty acids. In darkness, the loss of oxygen-evolving photosystem II activity in the presence of 0.5 M NaCl or 0.5 M LiCl was much more rapid in desA-/desD- cells than in wild-type cells. Oxygen-evolving activity that had been lost during incubation with 0.5 M NaCl in darkness returned when cells were transferred to conditions that allowed photosynthesis or respiration. Recovery was much greater in wild-type than in desA-/desD- cells, and it was prevented by lincomycin. Thus, the unsaturation of fatty acids is important in the tolerance of the photosynthetic machinery to salt stress. It appears also that the activity and synthesis of the Na+/H+ antiporter system might be suppressed under high-salt conditions and that this effect can be reversed, in part, by the unsaturation of fatty acids in membrane lipids.

KW - desA

KW - desD

KW - H antiport

KW - Na

KW - Photosystem II

KW - Salt tolerance

UR - http://www.scopus.com/inward/record.url?scp=0033545998&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033545998&partnerID=8YFLogxK

U2 - 10.1073/pnas.96.10.5862

DO - 10.1073/pnas.96.10.5862

M3 - Article

VL - 96

SP - 5862

EP - 5867

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 10

ER -