Genetic dissection of hormonal responses in the roots of arabidopsis grown under continuous mechanical impedance

Takashi Okamoto, Seiji Tsurumi, Kyohei Shibasaki, Yoshimi Obana, Hironori Takaji, Yutaka Oono, Abidur Rahman

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

We investigated the role of ethylene and auxin in regulating the growth and morphology of roots during mechanical impedance by developing a new growing system and using the model plant Arabidopsis (Arabidopsis thaliana). The Arabidopsis seedlings grown horizontally on a dialysis membrane-covered agar plate encountered adequate mechanical impedance as the roots showed characteristic ethylene phenotypes: 2-fold reduction in root growth, increase in root diameter, decrease in cell elongation, and ectopic root hair formation. The root phenotype characterization of various mutants having altered response to ethylene biosynthesis or signaling, the effect of ethylene inhibitors on mechanically impeded roots, and transcription profiling of the ethylene-responsive genes led us to conclude that enhanced ethylene response plays a primary role in changing root morphology and development during mechanical impedance. Further, the differential sensitivity of horizontally and vertically grown roots toward exogenous ethylene suggested that ethylene signaling plays a critical role in enhancing the ethylene response. We subsequently demonstrated that the enhanced ethylene response also affects the auxin response in roots. Taken together, our results provide a new insight into the role of ethylene in changing root morphology during mechanical impedance.

Original languageEnglish
Pages (from-to)1651-1662
Number of pages12
JournalPlant Physiology
Volume146
Issue number4
DOIs
Publication statusPublished - Apr 2008
Externally publishedYes

Fingerprint

impedance
Electric Impedance
Arabidopsis
Dissection
ethylene
Indoleacetic Acids
auxins
phenotype
ethylene inhibitors
Phenotype
root hairs
dialysis
ethylene production
cell growth
Growth
root growth
Seedlings
Arabidopsis thaliana
transcription (genetics)
agar

ASJC Scopus subject areas

  • Plant Science
  • Genetics
  • Physiology

Cite this

Genetic dissection of hormonal responses in the roots of arabidopsis grown under continuous mechanical impedance. / Okamoto, Takashi; Tsurumi, Seiji; Shibasaki, Kyohei; Obana, Yoshimi; Takaji, Hironori; Oono, Yutaka; Rahman, Abidur.

In: Plant Physiology, Vol. 146, No. 4, 04.2008, p. 1651-1662.

Research output: Contribution to journalArticle

Okamoto, Takashi ; Tsurumi, Seiji ; Shibasaki, Kyohei ; Obana, Yoshimi ; Takaji, Hironori ; Oono, Yutaka ; Rahman, Abidur. / Genetic dissection of hormonal responses in the roots of arabidopsis grown under continuous mechanical impedance. In: Plant Physiology. 2008 ; Vol. 146, No. 4. pp. 1651-1662.
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