Genetic control of startle behavior in medaka fish

Satomi Tsuboko, Tetsuaki Kimura, Minori Shinya, Yuji Suehiro, Teruhiro Okuyama, Atsuko Shimada, Hiroyuki Takeda, Kiyoshi Naruse, Takeo Kubo, Hideaki Takeuchi

Research output: Contribution to journalArticle

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Abstract

Genetic polymorphisms are thought to generate intraspecific behavioral diversities, both within and among populations. The mechanisms underlying genetic control of behavioral properties, however, remain unclear in wild-type vertebrates, including humans. To explore this issue, we used diverse inbred strains of medaka fish (Oryzias latipes) established from the same and different local populations. Medaka exhibit a startle response to a visual stimulus (extinction of illumination) by rapidly bending their bodies (C-start) 20-ms after the stimulus presentation. We measured the rates of the response to repeated stimuli (1-s interval, 40 times) among four inbred strains, HNI-I, HNI-II, HO5, and Hd-rR-II1, and quantified two properties of the startle response: sensitivity (response rate to the first stimulus) and attenuation of the response probability with repeated stimulus presentation. Among the four strains, the greatest differences in these properties were detected between HNI-II and Hd-rR-II1. HNI-II exhibited high sensitivity (approximately 80%) and no attenuation, while Hd-rR-II1 exhibited low sensitivity (approximately 50%) and almost complete attenuation after only five stimulus presentations. Our findings suggested behavioral diversity of the startle response within a local population as well as among different populations. Linkage analysis with F2 progeny between HNI-II and Hd-rR-II1 detected quantitative trait loci (QTL) highly related to attenuation, but not to sensitivity, with a maximum logarithm of odds score of 11.82 on linkage group 16. The three genotypes (homozygous for HNI-II and Hd-rR-II1 alleles, and heterozygous) at the marker nearest the QTL correlated with attenuation. Our findings are the first to suggest that a single genomic region might be sufficient to generate individual differences in startle behavior between wild-type strains. Further identification of genetic polymorphisms that define the behavioral trait will contribute to our understanding of the neural mechanisms underlying behavioral diversity, allowing us to investigate the adaptive significance of intraspecific behavioral polymorphisms of the startle response.

Original languageEnglish
Article number0112527
JournalPLoS One
Volume9
Issue number11
DOIs
Publication statusPublished - Nov 13 2014
Externally publishedYes

Fingerprint

Startle Reflex
Oryzias
Behavior Control
Polymorphism
Fish
Fishes
Quantitative Trait Loci
genetic polymorphism
Genetic Polymorphisms
fish
Population
Light extinction
quantitative trait loci
Behavioral Genetics
Oryzias latipes
homozygosity
Lighting
linkage groups
Individuality
linkage (genetics)

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Tsuboko, S., Kimura, T., Shinya, M., Suehiro, Y., Okuyama, T., Shimada, A., ... Takeuchi, H. (2014). Genetic control of startle behavior in medaka fish. PLoS One, 9(11), [0112527]. https://doi.org/10.1371/journal.pone.0112527

Genetic control of startle behavior in medaka fish. / Tsuboko, Satomi; Kimura, Tetsuaki; Shinya, Minori; Suehiro, Yuji; Okuyama, Teruhiro; Shimada, Atsuko; Takeda, Hiroyuki; Naruse, Kiyoshi; Kubo, Takeo; Takeuchi, Hideaki.

In: PLoS One, Vol. 9, No. 11, 0112527, 13.11.2014.

Research output: Contribution to journalArticle

Tsuboko, S, Kimura, T, Shinya, M, Suehiro, Y, Okuyama, T, Shimada, A, Takeda, H, Naruse, K, Kubo, T & Takeuchi, H 2014, 'Genetic control of startle behavior in medaka fish', PLoS One, vol. 9, no. 11, 0112527. https://doi.org/10.1371/journal.pone.0112527
Tsuboko S, Kimura T, Shinya M, Suehiro Y, Okuyama T, Shimada A et al. Genetic control of startle behavior in medaka fish. PLoS One. 2014 Nov 13;9(11). 0112527. https://doi.org/10.1371/journal.pone.0112527
Tsuboko, Satomi ; Kimura, Tetsuaki ; Shinya, Minori ; Suehiro, Yuji ; Okuyama, Teruhiro ; Shimada, Atsuko ; Takeda, Hiroyuki ; Naruse, Kiyoshi ; Kubo, Takeo ; Takeuchi, Hideaki. / Genetic control of startle behavior in medaka fish. In: PLoS One. 2014 ; Vol. 9, No. 11.
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