Genetic and epigenetic alteration among three homoeologous genes of a class E MADS box gene in hexaploid wheat

Naoki Shitsukawa, Chikako Tahira, Ken Ichiro Kassai, Chizuru Hirabayashi, Tomoaki Shimizu, Shigeo Takumi, Keiichi Mochida, Kanako Kawaura, Yasunari Ogihara, Koji Muraia

Research output: Contribution to journalArticle

107 Citations (Scopus)

Abstract

Bread wheat (Triticum aestivum) is a hexaploid species with A, B, and D ancestral genomes. Most bread wheat genes are present in the genome as triplicated homoeologous genes (homoeologs) derived from the ancestral species. Here, we report that both genetic and epigenetic alterations have occurred in the homoeologs of a wheat class E MADS box gene. Two class E genes are identified in wheat, wheat SEPALLATA (WSEP) and wheat LEAFY HULL STERILE1 (WLHS1), which are homologs of Os MADS45 and Os MADS1 in rice (Oryza sativa), respectively. The three wheat homoeologs of WSEP showed similar genomic structures and expression profiles. By contrast, the three homoeologs of WLHS1 showed genetic and epigenetic alterations. The A genome WLHS1 homoeolog (WLHS1-A) had a structural alteration that contained a large novel sequence in place of the K domain sequence. A yeast two-hybrid analysis and a transgenic experiment indicated that the WLHS1-A protein had no apparent function. The B and D genome homoeologs, WLHS1-B and WLHS1-D, respectively, had an intact MADS box gene structure, but WLHS1-B was predominantly silenced by cytosine methylation. Consequently, of the three WLHS1 homoeologs, only WLHS1-D functions in hexaploid wheat. This is a situation where three homoeologs are differentially regulated by genetic and epigenetic mechanisms.

Original languageEnglish
Pages (from-to)1723-1737
Number of pages15
JournalPlant Cell
Volume19
Issue number6
DOIs
Publication statusPublished - Jun 2007
Externally publishedYes

Fingerprint

hexaploidy
Epigenomics
epigenetics
Triticum
Genes
wheat
genes
Genome
genome
Bread
wheat classes
Methylation
two hybrid system techniques
Cytosine
cytosine
Yeast
methylation

ASJC Scopus subject areas

  • Plant Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Shitsukawa, N., Tahira, C., Kassai, K. I., Hirabayashi, C., Shimizu, T., Takumi, S., ... Muraia, K. (2007). Genetic and epigenetic alteration among three homoeologous genes of a class E MADS box gene in hexaploid wheat. Plant Cell, 19(6), 1723-1737. https://doi.org/10.1105/tpc.107.051813

Genetic and epigenetic alteration among three homoeologous genes of a class E MADS box gene in hexaploid wheat. / Shitsukawa, Naoki; Tahira, Chikako; Kassai, Ken Ichiro; Hirabayashi, Chizuru; Shimizu, Tomoaki; Takumi, Shigeo; Mochida, Keiichi; Kawaura, Kanako; Ogihara, Yasunari; Muraia, Koji.

In: Plant Cell, Vol. 19, No. 6, 06.2007, p. 1723-1737.

Research output: Contribution to journalArticle

Shitsukawa, N, Tahira, C, Kassai, KI, Hirabayashi, C, Shimizu, T, Takumi, S, Mochida, K, Kawaura, K, Ogihara, Y & Muraia, K 2007, 'Genetic and epigenetic alteration among three homoeologous genes of a class E MADS box gene in hexaploid wheat', Plant Cell, vol. 19, no. 6, pp. 1723-1737. https://doi.org/10.1105/tpc.107.051813
Shitsukawa N, Tahira C, Kassai KI, Hirabayashi C, Shimizu T, Takumi S et al. Genetic and epigenetic alteration among three homoeologous genes of a class E MADS box gene in hexaploid wheat. Plant Cell. 2007 Jun;19(6):1723-1737. https://doi.org/10.1105/tpc.107.051813
Shitsukawa, Naoki ; Tahira, Chikako ; Kassai, Ken Ichiro ; Hirabayashi, Chizuru ; Shimizu, Tomoaki ; Takumi, Shigeo ; Mochida, Keiichi ; Kawaura, Kanako ; Ogihara, Yasunari ; Muraia, Koji. / Genetic and epigenetic alteration among three homoeologous genes of a class E MADS box gene in hexaploid wheat. In: Plant Cell. 2007 ; Vol. 19, No. 6. pp. 1723-1737.
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