Genetic analysis of Vrn-B1 for vernalization requirement by using linked dCAPS markers in bread wheat (Triticum aestivum L.)

K. Iwaki, J. Nishida, T. Yanagisawa, H. Yoshida, Kenji Kato

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

To identify a molecular marker closely linked to Vrn-B1, the Vrn-1 ortholog on chromosome 5B, sequence polymorphism at four orthologous RFLP loci closely linked to the Vrn-1 gene family was analyzed by using near-isogenic lines of "Triple Dirk." At Xwg644, a RFLP locus, three types of nucleotide sequence differing by the number of (TG) repeats, two or three times, and base changes were detected. A (TG)3-type sequence proved to be specific to chromosome 5B by nulli-tetrasomic analysis, and substitution of single nucleotide (C/T) was detected between TD(B) carrying the former Vrn2 allele and TD(C) carrying the vrn2 allele. A mismatch primer was designed for dCAPS analysis of this single nucleotide polymorphism (SNP). Polymorphism was successfully detected between two NILs, through nested PCR by using a (TG)3-specific primer (1st) and a dCAPS primer (2nd) followed by a NsiI digest. The analysis of a BF2 population [(TD(B)//TD(C)] revealed the close linkage (1.7 cM) between WG644-5B and Vrn2. It was therefore concluded that the former Vrn2 locus is located on chromosome 5B and equivalent to Vrn-B1.

Original languageEnglish
Pages (from-to)571-576
Number of pages6
JournalTheoretical And Applied Genetics
Volume104
Issue number4
DOIs
Publication statusPublished - 2002

Fingerprint

vernalization
Bread
Triticum
genetic techniques and protocols
Triticum aestivum
Chromosomes
chromosomes
Restriction Fragment Length Polymorphisms
loci
wheat
restriction fragment length polymorphism
Alleles
genetic polymorphism
tetrasomics
alleles
isogenic lines
single nucleotide polymorphism
Single Nucleotide Polymorphism
linkage (genetics)
Nucleotides

Keywords

  • dCAPS
  • Orthologous gene
  • Synteny
  • Vernalization requirement
  • Wheat

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Horticulture
  • Plant Science
  • Genetics
  • Genetics(clinical)

Cite this

Genetic analysis of Vrn-B1 for vernalization requirement by using linked dCAPS markers in bread wheat (Triticum aestivum L.). / Iwaki, K.; Nishida, J.; Yanagisawa, T.; Yoshida, H.; Kato, Kenji.

In: Theoretical And Applied Genetics, Vol. 104, No. 4, 2002, p. 571-576.

Research output: Contribution to journalArticle

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