Genetic alterations of the putative envelope proteins encoding region of the hepatitis C virus in the progression to relapsed phase from acute hepatitis: Humoral immune response to hypervariable region 1

Hitomi Sekiya, Nobuyuki Kato, Yuko Ootsuyama, Takahide Nakazawa, Katsumi Yamauchi, Kunitada Shimotohno

Research output: Contribution to journalArticlepeer-review

48 Citations (Scopus)

Abstract

Hypervariable region I (HVRI) of the putative second envelope glycoprotein (gp70) of hepatitis C virus (HCV) undergoes sequential alterations at intervals of several months during the chronic phase of hepatitis. To evaluate the implications of sequence variability in HVRI of HCV, we investigated the sequence variability of the whole envelope‐protein(gp35 and gp70)‐coding regions of HCV genome derived from patient M in acute and relapsed phases (8‐month interval) of hepatitis. From this analysis, we found that a Leu (position 405) in HVRI substituted to Pro, and that 4 additional substitutions could be detected in gp70 during the relapsed phase. Sequence‐specific antibody against HVRI derived from patient M was first detected in the serum at 8 months after the onset of hepatitis, but no other specific antibodies against peptides containing aminoacid position(s) substituted in regions other than HVRI could be detected. Epitope mapping using the sequence of HVRI derived from the acute phase of hepatitis was also performed, and a B‐cell epitope (positions 397 to 407) of 11 amino acids was identified. However, the Pro variant at position 405 did not display an escape pattern from the antibody produced at 8 months after the onset. In addition, we demonstrated the existence of important a mi no‐acid residue positions which are recognized by the anti‐HVRI antibody produced in patient M using introduction point mutations within HVRI.

Original languageEnglish
Pages (from-to)664-670
Number of pages7
JournalInternational Journal of Cancer
Volume57
Issue number5
DOIs
Publication statusPublished - Jun 1 1994
Externally publishedYes

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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