Genetic alteration of penicillin non-susceptible Streptococcus pneumoniae observed throughout recurrence of acute otitis media detected by amplified fragment length polymorphism analysis

Ken Ichi Sugata, Kunihiro Fukushima, Teruhiro Ogawa, Tomoko Nakashima, Akemi Sugata, Norio Kasai, Mehmet Gunduz, Yasuyoshi Ueki, Kazunori Nishizaki

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Abstract

The prevalence of penicillin non-susceptible Streptococcus pneumoniae (PNSSP) is increasing among isolates from acute otitis media (AOM). Repeated episodes of antibiotic exposure are a well-known risk factor for the isolation of PNSSP although otitis-prone or recurrent AOM cases frequently require repeated courses of antibiotic treatment. In order to evaluate the chronological alteration of S. pneumoniae during recurrences of AOM, strains of S. pneumoniae were isolated from 11 patients, each of whom had experienced 2-4 episodes of AOM, were examined. Every bacterial specimen obtained from a single episode of recurrent AOM was examined by PCR-based penicillin-binding protein (PBP) assay, serotyping, and amplified fragment length polymorphism (AFLP), then compared to other samples from the same case. Two cases (18.2%) showed strain diversity during repeated antibiotic treatments by serotyping or PBP-assay. By AFLP analysis, 6 cases (54.5%) demonstrated heterogeneous strains during recurrent AOM. Clonal survivors of previous episodes of AOM were not always the cause of subsequent episodes of AOM, even in otitis-prone cases.

Original languageEnglish
Pages (from-to)167-174
Number of pages8
JournalActa Medica Okayama
Volume55
Issue number3
Publication statusPublished - 2001

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Amplified Fragment Length Polymorphism Analysis
Otitis Media
Streptococcus pneumoniae
Polymorphism
Penicillins
Penicillin-Binding Proteins
Anti-Bacterial Agents
Recurrence
Assays
Otitis
Serotyping
Survivors
Polymerase Chain Reaction

Keywords

  • Acute otitis media
  • Amplified fragment length polymorphism (AFLP)
  • Penicillin non-susceptible Streptococcus pneumoniae

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Genetic alteration of penicillin non-susceptible Streptococcus pneumoniae observed throughout recurrence of acute otitis media detected by amplified fragment length polymorphism analysis. / Sugata, Ken Ichi; Fukushima, Kunihiro; Ogawa, Teruhiro; Nakashima, Tomoko; Sugata, Akemi; Kasai, Norio; Gunduz, Mehmet; Ueki, Yasuyoshi; Nishizaki, Kazunori.

In: Acta Medica Okayama, Vol. 55, No. 3, 2001, p. 167-174.

Research output: Contribution to journalArticle

Sugata, Ken Ichi ; Fukushima, Kunihiro ; Ogawa, Teruhiro ; Nakashima, Tomoko ; Sugata, Akemi ; Kasai, Norio ; Gunduz, Mehmet ; Ueki, Yasuyoshi ; Nishizaki, Kazunori. / Genetic alteration of penicillin non-susceptible Streptococcus pneumoniae observed throughout recurrence of acute otitis media detected by amplified fragment length polymorphism analysis. In: Acta Medica Okayama. 2001 ; Vol. 55, No. 3. pp. 167-174.
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