Genes responsive to low-intensity pulsed ultrasound in MC3T3-E1 preosteoblast cells

Yoshiaki Tabuchi, Yuuki Sugahara, Mika Ikegame, Nobuo Suzuki, Kei ichiro Kitamura, Takashi Kondo

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)


Although low-intensity pulsed ultrasound (LIPUS) has been shown to enhance bone fracture healing, the underlying mechanism of LIPUS remains to be fully elucidated. Here, to better understand the molecular mechanism underlying cellular responses to LIPUS, we investigated gene expression profiles in mouse MC3T3-E1 preosteoblast cells exposed to LIPUS using high-density oligonucleotide microarrays and computational gene expression analysis tools. Although treatment of the cells with a single 20-min LIPUS (1.5 MHz, 30 mW/cm2) did not affect the cell growth or alkaline phosphatase activity, the treatment significantly increased the mRNA level of Bglap. Microarray analysis demonstrated that 38 genes were upregulated and 37 genes were downregulated by 1.5-fold or more in the cells at 24-h post-treatment. Ingenuity pathway analysis demonstrated that the gene network U (up) contained many upregulated genes that were mainly associated with bone morphology in the category of biological functions of skeletal and muscular system development and function. Moreover, the biological function of the gene network D (down), which contained downregulated genes, was associated with gene expression, the cell cycle and connective tissue development and function. These results should help to further clarify the molecular basis of the mechanisms of the LIPUS response in osteoblast cells.

Original languageEnglish
Pages (from-to)22721-22740
Number of pages20
JournalInternational journal of molecular sciences
Issue number11
Publication statusPublished - Nov 18 2013


  • Gene expression
  • Gene network
  • Low-intensity pulsed ultrasound
  • Microarray
  • Osteoblast

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry


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