Genes involved in novel adaptive aluminum resistance in Rhodotorula glutinis

Akio Tani, Takaya Kawahara, Yoko Yamamoto, Kazuhide Kimbara, Fusako Kawai

    Research output: Contribution to journalArticlepeer-review

    7 Citations (Scopus)

    Abstract

    Rhodotorula glutinis IFO1125 acquired increased aluminum (Al) resistance from 50 μM to more than 5 mM by repetitive culturing with stepwise increases in Al concentration at pH 4.0. In our previous study we performed differential display to find that three genes (RgFET3, RgGET3, and RgCMK) encoding proteins homologous to Saccharomyces cerevisiae FET3p, GET3p, and CMK1p and CMK2p, respectively, were up-regulated in the Al-resistant cells. In this study we cloned these genes and found they were indeed up-regulated in Al-resistant strains. The cloned genes were introduced into S. cerevisiae and corresponding mutants to test their relevance to Al resistance. The introduction of RgFET3 and RgGET3 conferred Al resistance to the host, but that of RgCMK did not. Green fluorescent protein (GFP)-tagged RgFet3p was localized at the cell periphery in the host. GFP-tagged RgGet3p formed more punctate bodies in the host under Al stress than in the absence of Al. Different growth responses to Fe (III), Cu (II), Ca ions, and cyclosporin A in the wild type and resistant cells of R. glutinis suggested the involvement and possible links of the three genes in Al resistance.

    Original languageEnglish
    Pages (from-to)453-458
    Number of pages6
    JournalJournal of Bioscience and Bioengineering
    Volume109
    Issue number5
    DOIs
    Publication statusPublished - May 2010

    Keywords

    • Aluminum resistance
    • FET3
    • GET3
    • Rhodotorula glutinis
    • calmodulin-dependent protein kinase (CMK)

    ASJC Scopus subject areas

    • Biotechnology
    • Bioengineering
    • Applied Microbiology and Biotechnology

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