TY - JOUR
T1 - Genes involved in novel adaptive aluminum resistance in Rhodotorula glutinis
AU - Tani, Akio
AU - Kawahara, Takaya
AU - Yamamoto, Yoko
AU - Kimbara, Kazuhide
AU - Kawai, Fusako
N1 - Funding Information:
This work was supported in part by GRANT-IN-AID FOR SCIENTIFIC RESEARCH (C) 17580065 from the Japan Society for the Promotion of Science and a grant for 2003 excellent research plan from the Research Institute of Innovative Technology for the Earth to F. K and A. T. This work was also supported by an aid to A. T. and Y. Y. from the Ohara Foundation. We are grateful to grants to F. K. from the Nissei Scientific Promotion Foundation and the Sumitomo Foundation.
PY - 2010/5
Y1 - 2010/5
N2 - Rhodotorula glutinis IFO1125 acquired increased aluminum (Al) resistance from 50 μM to more than 5 mM by repetitive culturing with stepwise increases in Al concentration at pH 4.0. In our previous study we performed differential display to find that three genes (RgFET3, RgGET3, and RgCMK) encoding proteins homologous to Saccharomyces cerevisiae FET3p, GET3p, and CMK1p and CMK2p, respectively, were up-regulated in the Al-resistant cells. In this study we cloned these genes and found they were indeed up-regulated in Al-resistant strains. The cloned genes were introduced into S. cerevisiae and corresponding mutants to test their relevance to Al resistance. The introduction of RgFET3 and RgGET3 conferred Al resistance to the host, but that of RgCMK did not. Green fluorescent protein (GFP)-tagged RgFet3p was localized at the cell periphery in the host. GFP-tagged RgGet3p formed more punctate bodies in the host under Al stress than in the absence of Al. Different growth responses to Fe (III), Cu (II), Ca ions, and cyclosporin A in the wild type and resistant cells of R. glutinis suggested the involvement and possible links of the three genes in Al resistance.
AB - Rhodotorula glutinis IFO1125 acquired increased aluminum (Al) resistance from 50 μM to more than 5 mM by repetitive culturing with stepwise increases in Al concentration at pH 4.0. In our previous study we performed differential display to find that three genes (RgFET3, RgGET3, and RgCMK) encoding proteins homologous to Saccharomyces cerevisiae FET3p, GET3p, and CMK1p and CMK2p, respectively, were up-regulated in the Al-resistant cells. In this study we cloned these genes and found they were indeed up-regulated in Al-resistant strains. The cloned genes were introduced into S. cerevisiae and corresponding mutants to test their relevance to Al resistance. The introduction of RgFET3 and RgGET3 conferred Al resistance to the host, but that of RgCMK did not. Green fluorescent protein (GFP)-tagged RgFet3p was localized at the cell periphery in the host. GFP-tagged RgGet3p formed more punctate bodies in the host under Al stress than in the absence of Al. Different growth responses to Fe (III), Cu (II), Ca ions, and cyclosporin A in the wild type and resistant cells of R. glutinis suggested the involvement and possible links of the three genes in Al resistance.
KW - Aluminum resistance
KW - FET3
KW - GET3
KW - Rhodotorula glutinis
KW - calmodulin-dependent protein kinase (CMK)
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U2 - 10.1016/j.jbiosc.2009.10.015
DO - 10.1016/j.jbiosc.2009.10.015
M3 - Article
C2 - 20347767
AN - SCOPUS:77949904569
VL - 109
SP - 453
EP - 458
JO - Journal of Bioscience and Bioengineering
JF - Journal of Bioscience and Bioengineering
SN - 1389-1723
IS - 5
ER -