Genes expression analyses of sea-island cotton (Gossypium barbadense L.) during fiber development

Li Li Tu, Xian Long Zhang, Shao Guang Liang, Di Qiu Liu, Long Fu Zhu, Fan Chang Zeng, Yi Chun Nie, Xiao Ping Guo, Feng Lin Deng, Jia Fu Tan, Li Xu

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

Sea-island cotton (Gossypium barbadense L.) is one of the most valuable cotton species due to its silkiness, luster, long staples, and high strength, but its fiber development mechanism has not been surveyed comprehensively. We constructed a normalized fiber cDNA library (from -2 to 25 dpa) of G. barbadense cv. Pima 3-79 (the genetic standard line) by saturation hybridization with genomic DNA. We screened Pima 3-79 fiber RNA from five developmental stages using a cDNA array including 9,126 plasmids randomly selected from the library, and we selected and sequenced 929 clones that had different signal intensities between any two stages. The 887 high-quality expressed sequence tags obtained were assembled into 645 consensus sequences (582 singletons and 63 contigs), of which 455 were assigned to functional categories using gene ontology. Almost 50% of binned genes belonged to metabolism functional categories. Based on subarray analysis of the 887 high-quality expressed sequence tags with 0-, 5-, 10-, 15-, and 20-dpa RNA of Pima 3-79 fibers and a mixture of RNA of nonfiber tissues, seven types of expression profiles were elucidated. Furthermore our results showed that phytohormones may play an important role in the fiber development.

Original languageEnglish
Pages (from-to)1309-1320
Number of pages12
JournalPlant Cell Reports
Volume26
Issue number8
DOIs
Publication statusPublished - Aug 2007
Externally publishedYes

Fingerprint

Gossypium barbadense
RNA
expressed sequence tags
gene expression
consensus sequence
staples
cDNA libraries
plant hormones
plasmids
hybridization
genes
cotton
developmental stages
clones
genomics
metabolism
DNA

Keywords

  • cDNA array
  • ESTs
  • Fiber development
  • Normalized cDNA library
  • Sea-island cotton

ASJC Scopus subject areas

  • Plant Science

Cite this

Tu, L. L., Zhang, X. L., Liang, S. G., Liu, D. Q., Zhu, L. F., Zeng, F. C., ... Xu, L. (2007). Genes expression analyses of sea-island cotton (Gossypium barbadense L.) during fiber development. Plant Cell Reports, 26(8), 1309-1320. https://doi.org/10.1007/s00299-007-0337-4

Genes expression analyses of sea-island cotton (Gossypium barbadense L.) during fiber development. / Tu, Li Li; Zhang, Xian Long; Liang, Shao Guang; Liu, Di Qiu; Zhu, Long Fu; Zeng, Fan Chang; Nie, Yi Chun; Guo, Xiao Ping; Deng, Feng Lin; Tan, Jia Fu; Xu, Li.

In: Plant Cell Reports, Vol. 26, No. 8, 08.2007, p. 1309-1320.

Research output: Contribution to journalArticle

Tu, LL, Zhang, XL, Liang, SG, Liu, DQ, Zhu, LF, Zeng, FC, Nie, YC, Guo, XP, Deng, FL, Tan, JF & Xu, L 2007, 'Genes expression analyses of sea-island cotton (Gossypium barbadense L.) during fiber development', Plant Cell Reports, vol. 26, no. 8, pp. 1309-1320. https://doi.org/10.1007/s00299-007-0337-4
Tu, Li Li ; Zhang, Xian Long ; Liang, Shao Guang ; Liu, Di Qiu ; Zhu, Long Fu ; Zeng, Fan Chang ; Nie, Yi Chun ; Guo, Xiao Ping ; Deng, Feng Lin ; Tan, Jia Fu ; Xu, Li. / Genes expression analyses of sea-island cotton (Gossypium barbadense L.) during fiber development. In: Plant Cell Reports. 2007 ; Vol. 26, No. 8. pp. 1309-1320.
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