Genes expressed in Ascochyta rabiei-inoculated chickpea plants and elicited cell cultures as detected by differential cDNA-hybridization

Yuki Ichinose, Karin Tiemann, Claudia Schwenger-Erger, Kazuhiro Toyoda, Frauke Hein, Thomas Hanselle, Holger Cornels, Wolfgang Barz

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

In response to the exogenous application of elicitors and attempted invasion by pathogens, plants exhibit a wide range of defense reactions. To understand the defense mechanisms at the level of gene activation and deactivation, differential screenings were performed to isolate cDNA clones which are differentially expressed in pathogen-inoculated resistant chickpea plants and elicitor-treated cell cultures. A plenty of genes were isolated and arranged in 5 groups, namely defense-related pathways, signal transduction pathways, regulation of gene expression, catabolic pathways and primary metabolism. Most of these genes were activated although several genes were also found to be suppressed. We discuss the plausible functions of cDNA products in plant defense responses. The cDNAs provide a variety of tools to investigate molecular mechanisms of defense responses and clearly reflect the massive genomic and metabolic changes which occur during manifestation of antimicrobial defense.

Original languageEnglish
Pages (from-to)44-54
Number of pages11
JournalZeitschrift fur Naturforschung - Section C Journal of Biosciences
Volume55
Issue number1-2
Publication statusPublished - Jan 2000

Fingerprint

Ascochyta rabiei
Cicer
Plant Cells
Cell culture
cell culture
hybridization
Complementary DNA
Cell Culture Techniques
Genes
defense mechanisms
Pathogens
gene activation
genes
plant products
gene expression regulation
Gene Expression Regulation
plant pathogens
Signal transduction
Transcriptional Activation
signal transduction

Keywords

  • Ascochyta rabiei
  • Cicer arietinum L.
  • Gene Activation
  • Gene Suppression
  • Plant Defence Genes

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

Cite this

Genes expressed in Ascochyta rabiei-inoculated chickpea plants and elicited cell cultures as detected by differential cDNA-hybridization. / Ichinose, Yuki; Tiemann, Karin; Schwenger-Erger, Claudia; Toyoda, Kazuhiro; Hein, Frauke; Hanselle, Thomas; Cornels, Holger; Barz, Wolfgang.

In: Zeitschrift fur Naturforschung - Section C Journal of Biosciences, Vol. 55, No. 1-2, 01.2000, p. 44-54.

Research output: Contribution to journalArticle

Ichinose, Yuki ; Tiemann, Karin ; Schwenger-Erger, Claudia ; Toyoda, Kazuhiro ; Hein, Frauke ; Hanselle, Thomas ; Cornels, Holger ; Barz, Wolfgang. / Genes expressed in Ascochyta rabiei-inoculated chickpea plants and elicited cell cultures as detected by differential cDNA-hybridization. In: Zeitschrift fur Naturforschung - Section C Journal of Biosciences. 2000 ; Vol. 55, No. 1-2. pp. 44-54.
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AU - Hanselle, Thomas

AU - Cornels, Holger

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