Generation of single-chain Fvs against detergent-solubilized recombinant antigens with a simple coating procedure

Torahiko Tanaka, Yuichiro Hasegawa, Makoto Saito, Masanori Ikeda, Nobuyuki Kato

Research output: Contribution to journalArticle

Abstract

Antigen coating on polystyrene is prevented by detergent. We present here a simple procedure to coat detergent-solubilized antigen for subsequent panning selection of single-chain Fv (scFv), the target antigen of which was the hepatitis C virus (HCV) non-structural protein (NS) 4B, an integral membrane protein.

Original languageEnglish
Pages (from-to)374-376
Number of pages3
JournalJournal of Bioscience and Bioengineering
Volume110
Issue number3
DOIs
Publication statusPublished - Sep 2010

Keywords

  • Critical micelle concentration
  • Detergent
  • Hepatitis C virus
  • NS4B
  • Single-chain Fv

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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