Generation of single-chain Fvs against detergent-solubilized recombinant antigens with a simple coating procedure

Torahiko Tanaka, Yuichiro Hasegawa, Makoto Saito, Masanori Ikeda, Nobuyuki Kato

Research output: Contribution to journalArticle

Abstract

Antigen coating on polystyrene is prevented by detergent. We present here a simple procedure to coat detergent-solubilized antigen for subsequent panning selection of single-chain Fv (scFv), the target antigen of which was the hepatitis C virus (HCV) non-structural protein (NS) 4B, an integral membrane protein.

Original languageEnglish
Pages (from-to)374-376
Number of pages3
JournalJournal of Bioscience and Bioengineering
Volume110
Issue number3
DOIs
Publication statusPublished - Sep 2010

Fingerprint

Detergents
Antigens
Coatings
Viral Structural Proteins
Proteins
Single-Chain Antibodies
Polystyrenes
Viruses
Hepacivirus
Membrane Proteins
Membranes

Keywords

  • Critical micelle concentration
  • Detergent
  • Hepatitis C virus
  • NS4B
  • Single-chain Fv

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Bioengineering

Cite this

Generation of single-chain Fvs against detergent-solubilized recombinant antigens with a simple coating procedure. / Tanaka, Torahiko; Hasegawa, Yuichiro; Saito, Makoto; Ikeda, Masanori; Kato, Nobuyuki.

In: Journal of Bioscience and Bioengineering, Vol. 110, No. 3, 09.2010, p. 374-376.

Research output: Contribution to journalArticle

Tanaka, Torahiko ; Hasegawa, Yuichiro ; Saito, Makoto ; Ikeda, Masanori ; Kato, Nobuyuki. / Generation of single-chain Fvs against detergent-solubilized recombinant antigens with a simple coating procedure. In: Journal of Bioscience and Bioengineering. 2010 ; Vol. 110, No. 3. pp. 374-376.
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