Generation of single-chain Fvs against detergent-solubilized recombinant antigens with a simple coating procedure

Torahiko Tanaka; Yuichiro Hasegawa; Makoto Saito; Masanori Ikeda; Nobuyuki Kato

doi:10.1016/j.jbiosc.2010.03.014

Generation of single-chain Fvs against detergent-solubilized recombinant antigens with a simple coating procedure

Torahiko Tanaka, Yuichiro Hasegawa, Makoto Saito, Masanori Ikeda, Nobuyuki Kato

Research output: Contribution to journal › Article › peer-review

Abstract

Antigen coating on polystyrene is prevented by detergent. We present here a simple procedure to coat detergent-solubilized antigen for subsequent panning selection of single-chain Fv (scFv), the target antigen of which was the hepatitis C virus (HCV) non-structural protein (NS) 4B, an integral membrane protein.

Original language	English
Pages (from-to)	374-376
Number of pages	3
Journal	Journal of Bioscience and Bioengineering
Volume	110
Issue number	3
DOIs	https://doi.org/10.1016/j.jbiosc.2010.03.014
Publication status	Published - Sep 2010
Externally published	Yes

Keywords

Critical micelle concentration
Detergent
Hepatitis C virus
NS4B
Single-chain Fv

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

UN SDGs

This output contributes to the following Sustainable Development Goal(s)

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10.1016/j.jbiosc.2010.03.014

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title = "Generation of single-chain Fvs against detergent-solubilized recombinant antigens with a simple coating procedure",

abstract = "Antigen coating on polystyrene is prevented by detergent. We present here a simple procedure to coat detergent-solubilized antigen for subsequent panning selection of single-chain Fv (scFv), the target antigen of which was the hepatitis C virus (HCV) non-structural protein (NS) 4B, an integral membrane protein.",

keywords = "Critical micelle concentration, Detergent, Hepatitis C virus, NS4B, Single-chain Fv",

author = "Torahiko Tanaka and Yuichiro Hasegawa and Makoto Saito and Masanori Ikeda and Nobuyuki Kato",

note = "Funding Information: This work was supported by a Nihon University Joint Research Grant, a grant from the Ministry of Education, Culture, Sports, Science, and Technology to promote open research for young academics and specialists, and the grant “Strategic Research Base Development” Program for Private Universities subsidized by MEXT (2008). We thank Dr. T. Hayashida (Northwestern University) for suggestions.",

year = "2010",

month = sep,

doi = "10.1016/j.jbiosc.2010.03.014",

language = "English",

volume = "110",

pages = "374--376",

journal = "Journal of Bioscience and Bioengineering",

issn = "1389-1723",

publisher = "The Society for Biotechnology, Japan",

number = "3",

}

TY - JOUR

T1 - Generation of single-chain Fvs against detergent-solubilized recombinant antigens with a simple coating procedure

AU - Tanaka, Torahiko

AU - Hasegawa, Yuichiro

AU - Saito, Makoto

AU - Ikeda, Masanori

AU - Kato, Nobuyuki

N1 - Funding Information: This work was supported by a Nihon University Joint Research Grant, a grant from the Ministry of Education, Culture, Sports, Science, and Technology to promote open research for young academics and specialists, and the grant “Strategic Research Base Development” Program for Private Universities subsidized by MEXT (2008). We thank Dr. T. Hayashida (Northwestern University) for suggestions.

PY - 2010/9

Y1 - 2010/9

N2 - Antigen coating on polystyrene is prevented by detergent. We present here a simple procedure to coat detergent-solubilized antigen for subsequent panning selection of single-chain Fv (scFv), the target antigen of which was the hepatitis C virus (HCV) non-structural protein (NS) 4B, an integral membrane protein.

AB - Antigen coating on polystyrene is prevented by detergent. We present here a simple procedure to coat detergent-solubilized antigen for subsequent panning selection of single-chain Fv (scFv), the target antigen of which was the hepatitis C virus (HCV) non-structural protein (NS) 4B, an integral membrane protein.

KW - Critical micelle concentration

KW - Detergent

KW - Hepatitis C virus

KW - NS4B

KW - Single-chain Fv

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U2 - 10.1016/j.jbiosc.2010.03.014

DO - 10.1016/j.jbiosc.2010.03.014

M3 - Article

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VL - 110

SP - 374

EP - 376

JO - Journal of Bioscience and Bioengineering

JF - Journal of Bioscience and Bioengineering

SN - 1389-1723

IS - 3

ER -

Generation of single-chain Fvs against detergent-solubilized recombinant antigens with a simple coating procedure

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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