Generation of single-chain Fvs against detergent-solubilized recombinant antigens with a simple coating procedure

Torahiko Tanaka; Yuichiro Hasegawa; Makoto Saito; Masanori Ikeda; Nobuyuki Kato

doi:10.1016/j.jbiosc.2010.03.014

Generation of single-chain Fvs against detergent-solubilized recombinant antigens with a simple coating procedure

Torahiko Tanaka, Yuichiro Hasegawa, Makoto Saito, Masanori Ikeda, Nobuyuki Kato

Research output: Contribution to journal › Article

Abstract

Antigen coating on polystyrene is prevented by detergent. We present here a simple procedure to coat detergent-solubilized antigen for subsequent panning selection of single-chain Fv (scFv), the target antigen of which was the hepatitis C virus (HCV) non-structural protein (NS) 4B, an integral membrane protein.

Original language	English
Pages (from-to)	374-376
Number of pages	3
Journal	Journal of Bioscience and Bioengineering
Volume	110
Issue number	3
DOIs	https://doi.org/10.1016/j.jbiosc.2010.03.014
Publication status	Published - Sep 2010

Keywords

Critical micelle concentration
Detergent
Hepatitis C virus
NS4B
Single-chain Fv

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

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Tanaka, T., Hasegawa, Y., Saito, M., Ikeda, M., & Kato, N. (2010). Generation of single-chain Fvs against detergent-solubilized recombinant antigens with a simple coating procedure. Journal of Bioscience and Bioengineering, 110(3), 374-376. https://doi.org/10.1016/j.jbiosc.2010.03.014

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abstract = "Antigen coating on polystyrene is prevented by detergent. We present here a simple procedure to coat detergent-solubilized antigen for subsequent panning selection of single-chain Fv (scFv), the target antigen of which was the hepatitis C virus (HCV) non-structural protein (NS) 4B, an integral membrane protein.",

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author = "Torahiko Tanaka and Yuichiro Hasegawa and Makoto Saito and Masanori Ikeda and Nobuyuki Kato",

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AU - Saito, Makoto

AU - Ikeda, Masanori

AU - Kato, Nobuyuki

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KW - Hepatitis C virus

KW - NS4B

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