Antigen coating on polystyrene is prevented by detergent. We present here a simple procedure to coat detergent-solubilized antigen for subsequent panning selection of single-chain Fv (scFv), the target antigen of which was the hepatitis C virus (HCV) non-structural protein (NS) 4B, an integral membrane protein.
- Critical micelle concentration
- Hepatitis C virus
- Single-chain Fv
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology