Abstract
Antigen coating on polystyrene is prevented by detergent. We present here a simple procedure to coat detergent-solubilized antigen for subsequent panning selection of single-chain Fv (scFv), the target antigen of which was the hepatitis C virus (HCV) non-structural protein (NS) 4B, an integral membrane protein.
Original language | English |
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Pages (from-to) | 374-376 |
Number of pages | 3 |
Journal | Journal of Bioscience and Bioengineering |
Volume | 110 |
Issue number | 3 |
DOIs | |
Publication status | Published - Sep 2010 |
Keywords
- Critical micelle concentration
- Detergent
- Hepatitis C virus
- NS4B
- Single-chain Fv
ASJC Scopus subject areas
- Biotechnology
- Bioengineering
- Applied Microbiology and Biotechnology