Abstract
Antigen coating on polystyrene is prevented by detergent. We present here a simple procedure to coat detergent-solubilized antigen for subsequent panning selection of single-chain Fv (scFv), the target antigen of which was the hepatitis C virus (HCV) non-structural protein (NS) 4B, an integral membrane protein.
| Original language | English |
|---|---|
| Pages (from-to) | 374-376 |
| Number of pages | 3 |
| Journal | Journal of Bioscience and Bioengineering |
| Volume | 110 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Sep 2010 |
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Keywords
- Critical micelle concentration
- Detergent
- Hepatitis C virus
- NS4B
- Single-chain Fv
ASJC Scopus subject areas
- Biotechnology
- Applied Microbiology and Biotechnology
- Bioengineering
Cite this
Generation of single-chain Fvs against detergent-solubilized recombinant antigens with a simple coating procedure. / Tanaka, Torahiko; Hasegawa, Yuichiro; Saito, Makoto; Ikeda, Masanori; Kato, Nobuyuki.
In: Journal of Bioscience and Bioengineering, Vol. 110, No. 3, 09.2010, p. 374-376.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Generation of single-chain Fvs against detergent-solubilized recombinant antigens with a simple coating procedure
AU - Tanaka, Torahiko
AU - Hasegawa, Yuichiro
AU - Saito, Makoto
AU - Ikeda, Masanori
AU - Kato, Nobuyuki
PY - 2010/9
Y1 - 2010/9
N2 - Antigen coating on polystyrene is prevented by detergent. We present here a simple procedure to coat detergent-solubilized antigen for subsequent panning selection of single-chain Fv (scFv), the target antigen of which was the hepatitis C virus (HCV) non-structural protein (NS) 4B, an integral membrane protein.
AB - Antigen coating on polystyrene is prevented by detergent. We present here a simple procedure to coat detergent-solubilized antigen for subsequent panning selection of single-chain Fv (scFv), the target antigen of which was the hepatitis C virus (HCV) non-structural protein (NS) 4B, an integral membrane protein.
KW - Critical micelle concentration
KW - Detergent
KW - Hepatitis C virus
KW - NS4B
KW - Single-chain Fv
UR - http://www.scopus.com/inward/record.url?scp=77955314157&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77955314157&partnerID=8YFLogxK
U2 - 10.1016/j.jbiosc.2010.03.014
DO - 10.1016/j.jbiosc.2010.03.014
M3 - Article
C2 - 20547361
AN - SCOPUS:77955314157
VL - 110
SP - 374
EP - 376
JO - Journal of Bioscience and Bioengineering
JF - Journal of Bioscience and Bioengineering
SN - 1389-1723
IS - 3
ER -