Generation of colonic IgA-secreting cells in the caecal patch

Kazunori Masahata; Eiji Umemoto; Hisako Kayama; Manato Kotani; Shota Nakamura; Takashi Kurakawa; Junichi Kikuta; Kazuyoshi Gotoh; Daisuke Motooka; Shintaro Sato; Tomonori Higuchi; Yoshihiro Baba; Tomohiro Kurosaki; Makoto Kinoshita; Yosuke Shimada; Taishi Kimura; Ryu Okumura; Akira Takeda; Masaru Tajima; Osamu Yoshie; Masahiro Fukuzawa; Hiroshi Kiyono; Sidonia Fagarasan; Tetsuya Iida; Masaru Ishii; Kiyoshi Takeda

doi:10.1038/ncomms4704

Generation of colonic IgA-secreting cells in the caecal patch

Kazunori Masahata, Eiji Umemoto, Hisako Kayama, Manato Kotani, Shota Nakamura, Takashi Kurakawa, Junichi Kikuta, Kazuyoshi Gotoh, Daisuke Motooka, Shintaro Sato, Tomonori Higuchi, Yoshihiro Baba, Tomohiro Kurosaki, Makoto Kinoshita, Yosuke Shimada, Taishi Kimura, Ryu Okumura, Akira Takeda, Masaru Tajima, Osamu YoshieMasahiro Fukuzawa, Hiroshi Kiyono, Sidonia Fagarasan, Tetsuya Iida, Masaru Ishii, Kiyoshi Takeda

Research output: Contribution to journal › Article › peer-review

91 Citations (Scopus)

Abstract

Gut-associated lymphoid tissues are responsible for the generation of IgA-secreting cells. However, the function of the caecal patch, a lymphoid tissue in the appendix, remains unknown. Here we analyse the role of the caecal patch using germ-free mice colonized with intestinal bacteria after appendectomy. Appendectomized mice show delayed accumulation of IgA(+) cells in the large intestine, but not the small intestine, after colonization. Decreased colonic IgA(+) cells correlate with altered faecal microbiota composition. Experiments using photoconvertible Kaede-expressing mice or adoptive transfer show that the caecal patch IgA(+) cells migrate to the large and small intestines, whereas Peyer's patch cells are preferentially recruited to the small intestine. IgA(+) cells in the caecal patch express higher levels of CCR10. Dendritic cells in the caecal patch, but not Peyer's patches, induce CCR10 on cocultured B cells. Thus, the caecal patch is a major site for generation of IgA-secreting cells that migrate to the large intestine.

Original language	English
Article number	3704
Pages (from-to)	3704
Number of pages	1
Journal	Nature communications
Volume	5
DOIs	https://doi.org/10.1038/ncomms4704
Publication status	Published - 2014
Externally published	Yes

ASJC Scopus subject areas

Chemistry(all)
Biochemistry, Genetics and Molecular Biology(all)
Physics and Astronomy(all)

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10.1038/ncomms4704

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Masahata, K., Umemoto, E., Kayama, H., Kotani, M., Nakamura, S., Kurakawa, T., Kikuta, J., Gotoh, K., Motooka, D., Sato, S., Higuchi, T., Baba, Y., Kurosaki, T., Kinoshita, M., Shimada, Y., Kimura, T., Okumura, R., Takeda, A., Tajima, M., ... Takeda, K. (2014). Generation of colonic IgA-secreting cells in the caecal patch. Nature communications, 5, 3704. [3704]. https://doi.org/10.1038/ncomms4704

Masahata, K, Umemoto, E, Kayama, H, Kotani, M, Nakamura, S, Kurakawa, T, Kikuta, J, Gotoh, K, Motooka, D, Sato, S, Higuchi, T, Baba, Y, Kurosaki, T, Kinoshita, M, Shimada, Y, Kimura, T, Okumura, R, Takeda, A, Tajima, M, Yoshie, O, Fukuzawa, M, Kiyono, H, Fagarasan, S, Iida, T, Ishii, M & Takeda, K 2014, 'Generation of colonic IgA-secreting cells in the caecal patch', Nature communications, vol. 5, 3704, pp. 3704. https://doi.org/10.1038/ncomms4704

@article{3c6ab87843a0477c96411aab84589d13,

title = "Generation of colonic IgA-secreting cells in the caecal patch",

abstract = "Gut-associated lymphoid tissues are responsible for the generation of IgA-secreting cells. However, the function of the caecal patch, a lymphoid tissue in the appendix, remains unknown. Here we analyse the role of the caecal patch using germ-free mice colonized with intestinal bacteria after appendectomy. Appendectomized mice show delayed accumulation of IgA(+) cells in the large intestine, but not the small intestine, after colonization. Decreased colonic IgA(+) cells correlate with altered faecal microbiota composition. Experiments using photoconvertible Kaede-expressing mice or adoptive transfer show that the caecal patch IgA(+) cells migrate to the large and small intestines, whereas Peyer's patch cells are preferentially recruited to the small intestine. IgA(+) cells in the caecal patch express higher levels of CCR10. Dendritic cells in the caecal patch, but not Peyer's patches, induce CCR10 on cocultured B cells. Thus, the caecal patch is a major site for generation of IgA-secreting cells that migrate to the large intestine. ",

author = "Kazunori Masahata and Eiji Umemoto and Hisako Kayama and Manato Kotani and Shota Nakamura and Takashi Kurakawa and Junichi Kikuta and Kazuyoshi Gotoh and Daisuke Motooka and Shintaro Sato and Tomonori Higuchi and Yoshihiro Baba and Tomohiro Kurosaki and Makoto Kinoshita and Yosuke Shimada and Taishi Kimura and Ryu Okumura and Akira Takeda and Masaru Tajima and Osamu Yoshie and Masahiro Fukuzawa and Hiroshi Kiyono and Sidonia Fagarasan and Tetsuya Iida and Masaru Ishii and Kiyoshi Takeda",

note = "Funding Information: We thank M. Tomura for providing us with Kaede-transgenic mice, M. Maruya for the analysis of IgA-secreting cells, T. Kondo and Y. Magota for the maintenance of the germfree mice and C. Hidaka for secretarial assistance. This work was supported by grants from the Ministry of Education, Culture, Sports, Science and Technology, the Japan Science and Technology Agency, and by the Ministry of Health, Labour and Welfare and The Osaka Foundation for Promotion of Clinical Immunology.",

year = "2014",

doi = "10.1038/ncomms4704",

language = "English",

volume = "5",

pages = "3704",

journal = "Nature Communications",

issn = "2041-1723",

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T1 - Generation of colonic IgA-secreting cells in the caecal patch

AU - Masahata, Kazunori

AU - Umemoto, Eiji

AU - Kayama, Hisako

AU - Kotani, Manato

AU - Nakamura, Shota

AU - Kurakawa, Takashi

AU - Kikuta, Junichi

AU - Gotoh, Kazuyoshi

AU - Motooka, Daisuke

AU - Sato, Shintaro

AU - Higuchi, Tomonori

AU - Baba, Yoshihiro

AU - Kurosaki, Tomohiro

AU - Kinoshita, Makoto

AU - Shimada, Yosuke

AU - Kimura, Taishi

AU - Okumura, Ryu

AU - Takeda, Akira

AU - Tajima, Masaru

AU - Yoshie, Osamu

AU - Fukuzawa, Masahiro

AU - Kiyono, Hiroshi

AU - Fagarasan, Sidonia

AU - Iida, Tetsuya

AU - Ishii, Masaru

AU - Takeda, Kiyoshi

N1 - Funding Information: We thank M. Tomura for providing us with Kaede-transgenic mice, M. Maruya for the analysis of IgA-secreting cells, T. Kondo and Y. Magota for the maintenance of the germfree mice and C. Hidaka for secretarial assistance. This work was supported by grants from the Ministry of Education, Culture, Sports, Science and Technology, the Japan Science and Technology Agency, and by the Ministry of Health, Labour and Welfare and The Osaka Foundation for Promotion of Clinical Immunology.

PY - 2014

Y1 - 2014

N2 - Gut-associated lymphoid tissues are responsible for the generation of IgA-secreting cells. However, the function of the caecal patch, a lymphoid tissue in the appendix, remains unknown. Here we analyse the role of the caecal patch using germ-free mice colonized with intestinal bacteria after appendectomy. Appendectomized mice show delayed accumulation of IgA(+) cells in the large intestine, but not the small intestine, after colonization. Decreased colonic IgA(+) cells correlate with altered faecal microbiota composition. Experiments using photoconvertible Kaede-expressing mice or adoptive transfer show that the caecal patch IgA(+) cells migrate to the large and small intestines, whereas Peyer's patch cells are preferentially recruited to the small intestine. IgA(+) cells in the caecal patch express higher levels of CCR10. Dendritic cells in the caecal patch, but not Peyer's patches, induce CCR10 on cocultured B cells. Thus, the caecal patch is a major site for generation of IgA-secreting cells that migrate to the large intestine.

AB - Gut-associated lymphoid tissues are responsible for the generation of IgA-secreting cells. However, the function of the caecal patch, a lymphoid tissue in the appendix, remains unknown. Here we analyse the role of the caecal patch using germ-free mice colonized with intestinal bacteria after appendectomy. Appendectomized mice show delayed accumulation of IgA(+) cells in the large intestine, but not the small intestine, after colonization. Decreased colonic IgA(+) cells correlate with altered faecal microbiota composition. Experiments using photoconvertible Kaede-expressing mice or adoptive transfer show that the caecal patch IgA(+) cells migrate to the large and small intestines, whereas Peyer's patch cells are preferentially recruited to the small intestine. IgA(+) cells in the caecal patch express higher levels of CCR10. Dendritic cells in the caecal patch, but not Peyer's patches, induce CCR10 on cocultured B cells. Thus, the caecal patch is a major site for generation of IgA-secreting cells that migrate to the large intestine.

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DO - 10.1038/ncomms4704

M3 - Article

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SN - 2041-1723

VL - 5

SP - 3704

JO - Nature Communications

JF - Nature Communications

M1 - 3704

ER -

Generation of colonic IgA-secreting cells in the caecal patch

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