Generation of cell-permeable artificial zinc finger protein variants

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Citations (Scopus)

Abstract

Designed or artificial zinc finger proteins (ZFPs) are one of the most promising DNA-binding proteins that target genomic sequences of interest in vitro and in vivo. Conjugation of other functional domains such as transcriptional regulatory domains and endonucleases to ZFPs provided powerful molecular tools to modulate endogenous gene expression and genetic information. These ZFP variants have been introduced into cells as DNA-encoding ZFP variants by using plasmids or viral vectors. As an alternative delivery method of ZFP variants, we developed cell-permeable ZFP variants by fusing cell-penetrating peptides to ZFP variants. We will describe how to generate cell-permeable artificial ZFP variants and how to examine the cell permeabilities by immunofluorescent staining.

Original languageEnglish
Title of host publicationEngineered Zinc Finger Proteins
Subtitle of host publicationMethods and Protocols
EditorsJoel Mackay, David Segal
Pages91-96
Number of pages6
DOIs
Publication statusPublished - Dec 1 2010
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume649
ISSN (Print)1064-3745

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Keywords

  • Zinc finger protein
  • cell permeability
  • cell-penetrating peptide
  • immunofluorescent staining
  • ion-exchange chromatography

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Sera, T. (2010). Generation of cell-permeable artificial zinc finger protein variants. In J. Mackay, & D. Segal (Eds.), Engineered Zinc Finger Proteins: Methods and Protocols (pp. 91-96). (Methods in Molecular Biology; Vol. 649). https://doi.org/10.1007/978-1-60761-753-2_5