TY - CHAP
T1 - Generation of cell-permeable artificial zinc finger protein variants
AU - Sera, Takashi
PY - 2010/12/1
Y1 - 2010/12/1
N2 - Designed or artificial zinc finger proteins (ZFPs) are one of the most promising DNA-binding proteins that target genomic sequences of interest in vitro and in vivo. Conjugation of other functional domains such as transcriptional regulatory domains and endonucleases to ZFPs provided powerful molecular tools to modulate endogenous gene expression and genetic information. These ZFP variants have been introduced into cells as DNA-encoding ZFP variants by using plasmids or viral vectors. As an alternative delivery method of ZFP variants, we developed cell-permeable ZFP variants by fusing cell-penetrating peptides to ZFP variants. We will describe how to generate cell-permeable artificial ZFP variants and how to examine the cell permeabilities by immunofluorescent staining.
AB - Designed or artificial zinc finger proteins (ZFPs) are one of the most promising DNA-binding proteins that target genomic sequences of interest in vitro and in vivo. Conjugation of other functional domains such as transcriptional regulatory domains and endonucleases to ZFPs provided powerful molecular tools to modulate endogenous gene expression and genetic information. These ZFP variants have been introduced into cells as DNA-encoding ZFP variants by using plasmids or viral vectors. As an alternative delivery method of ZFP variants, we developed cell-permeable ZFP variants by fusing cell-penetrating peptides to ZFP variants. We will describe how to generate cell-permeable artificial ZFP variants and how to examine the cell permeabilities by immunofluorescent staining.
KW - Zinc finger protein
KW - cell permeability
KW - cell-penetrating peptide
KW - immunofluorescent staining
KW - ion-exchange chromatography
UR - http://www.scopus.com/inward/record.url?scp=79953136920&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79953136920&partnerID=8YFLogxK
U2 - 10.1007/978-1-60761-753-2_5
DO - 10.1007/978-1-60761-753-2_5
M3 - Chapter
C2 - 20680829
AN - SCOPUS:79953136920
SN - 9781607617525
T3 - Methods in Molecular Biology
SP - 91
EP - 96
BT - Engineered Zinc Finger Proteins
A2 - Mackay, Joel
A2 - Segal, David
ER -