Abstract
Designed or artificial zinc finger proteins (ZFPs) are one of the most promising DNA-binding proteins that target genomic sequences of interest in vitro and in vivo. Conjugation of other functional domains such as transcriptional regulatory domains and endonucleases to ZFPs provided powerful molecular tools to modulate endogenous gene expression and genetic information. These ZFP variants have been introduced into cells as DNA-encoding ZFP variants by using plasmids or viral vectors. As an alternative delivery method of ZFP variants, we developed cell-permeable ZFP variants by fusing cell-penetrating peptides to ZFP variants. We will describe how to generate cell-permeable artificial ZFP variants and how to examine the cell permeabilities by immunofluorescent staining.
| Original language | English |
|---|---|
| Title of host publication | Methods in Molecular Biology |
| Pages | 91-96 |
| Number of pages | 6 |
| Volume | 649 |
| DOIs | |
| Publication status | Published - 2010 |
| Externally published | Yes |
Publication series
| Name | Methods in Molecular Biology |
|---|---|
| Volume | 649 |
| ISSN (Print) | 10643745 |
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Keywords
- cell permeability
- cell-penetrating peptide
- immunofluorescent staining
- ion-exchange chromatography
- Zinc finger protein
ASJC Scopus subject areas
- Molecular Biology
- Genetics
- Medicine(all)
Cite this
Generation of cell-permeable artificial zinc finger protein variants. / Sera, Takashi.
Methods in Molecular Biology. Vol. 649 2010. p. 91-96 (Methods in Molecular Biology; Vol. 649).Research output: Chapter in Book/Report/Conference proceeding › Chapter
}
TY - CHAP
T1 - Generation of cell-permeable artificial zinc finger protein variants
AU - Sera, Takashi
PY - 2010
Y1 - 2010
N2 - Designed or artificial zinc finger proteins (ZFPs) are one of the most promising DNA-binding proteins that target genomic sequences of interest in vitro and in vivo. Conjugation of other functional domains such as transcriptional regulatory domains and endonucleases to ZFPs provided powerful molecular tools to modulate endogenous gene expression and genetic information. These ZFP variants have been introduced into cells as DNA-encoding ZFP variants by using plasmids or viral vectors. As an alternative delivery method of ZFP variants, we developed cell-permeable ZFP variants by fusing cell-penetrating peptides to ZFP variants. We will describe how to generate cell-permeable artificial ZFP variants and how to examine the cell permeabilities by immunofluorescent staining.
AB - Designed or artificial zinc finger proteins (ZFPs) are one of the most promising DNA-binding proteins that target genomic sequences of interest in vitro and in vivo. Conjugation of other functional domains such as transcriptional regulatory domains and endonucleases to ZFPs provided powerful molecular tools to modulate endogenous gene expression and genetic information. These ZFP variants have been introduced into cells as DNA-encoding ZFP variants by using plasmids or viral vectors. As an alternative delivery method of ZFP variants, we developed cell-permeable ZFP variants by fusing cell-penetrating peptides to ZFP variants. We will describe how to generate cell-permeable artificial ZFP variants and how to examine the cell permeabilities by immunofluorescent staining.
KW - cell permeability
KW - cell-penetrating peptide
KW - immunofluorescent staining
KW - ion-exchange chromatography
KW - Zinc finger protein
UR - http://www.scopus.com/inward/record.url?scp=79953136920&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79953136920&partnerID=8YFLogxK
U2 - 10.1007/978-1-60761-753-2_5
DO - 10.1007/978-1-60761-753-2_5
M3 - Chapter
SN - 9781607617525
VL - 649
T3 - Methods in Molecular Biology
SP - 91
EP - 96
BT - Methods in Molecular Biology
ER -