Generation of active fragments from human zymogens in the brady kinin-generating cascade by extracellular proteases from Vibrio vulnificus and V. parahaemolyticus

Shin Ichi Miyoshi, Hirofumi Watanabe, Tomoka Kawase, Hidenori Yamada, Sumio Shinoda

Research output: Contribution to journalArticlepeer-review

22 Citations (Scopus)

Abstract

Vibrio vulnificus is an opportunistic human pathogen causing septicemia, and the infection is characterized by formation of the edematous skin lesions on limbs. This pathogenic species secretes a thermolysin-like metalloprotease as a virulence determinant. The metalloprotease was confirmed to activate human factor XII-plasma kallikrein-kinin cascade that results in liberation of bradykinin, a chemical mediator enhancing the vascular permeability, from high-molecular weight kininogen. Namely, the metalloprotease showed to generate active fragments by cleavage of Arg-Ile, Arg-Val or Gly-Leu peptide bond in human zymogens (plasma prekallikrein and factor XII). In spite of induction of the sufficient vascular permeability-enhancing and edema-forming reaction in the guinea pig model, a serine protease from V. parahaemolyticus, a human pathogen causing primarily watery diarrhea, showed far less ability to activate and to cleave the human zymogens. These results in part may explain why only V. vulnificus often causes serious edematous skin damages in humans.

Original languageEnglish
Pages (from-to)887-893
Number of pages7
JournalToxicon
Volume44
Issue number8
DOIs
Publication statusPublished - Dec 15 2004

Keywords

  • Factor XII, Plasma prekallikrein
  • Protease
  • Vibrio parahaemolyticus
  • Vibrio vulnificus

ASJC Scopus subject areas

  • Toxicology

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