Generation of a bioengineered tooth by using a three-dimensional cell manipulation method (Organ Germ Method)

Masamitsu Oshima, Miho Ogawa, Masato Yasukawa, Takashi Tsuji

Research output: Chapter in Book/Report/Conference proceedingChapter

11 Citations (Scopus)

Abstract

The arrangement of cells within a tissue plays an essential role in organogenesis, including tooth development. Organ morphogenesis and physiological functions induced by three-dimensional tissue organization are well known to be regulated by the proper spatiotemporal organization of various signaling molecules, including cytokines, extracellular matrix proteins, and adhesion molecules. Development of a three-dimensional cell manipulation technology to create a bioengineered organ germ, designated as the organ germ method, enabled the generation of a structurally correct and fully functional bioengineered tooth in vivo. This method is expected to be utilized as a valuable technique for analyzing gene and protein functions during organogenesis. Here, we describe protocols for tooth germ reconstitution using the organ germ method and methods for analyzing tooth development in vitro and in vivo.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages149-165
Number of pages17
Volume887
ISBN (Print)9781617798597
DOIs
Publication statusPublished - 2012
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume887
ISSN (Print)10643745

Fingerprint

Tooth
Organogenesis
Tooth Germ
Extracellular Matrix Proteins
Morphogenesis
Cytokines
Technology
Proteins

Keywords

  • Bioengineered tooth
  • Cell manipulation
  • Organ germ method
  • Tooth germ
  • Transplantation

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Medicine(all)

Cite this

Oshima, M., Ogawa, M., Yasukawa, M., & Tsuji, T. (2012). Generation of a bioengineered tooth by using a three-dimensional cell manipulation method (Organ Germ Method). In Methods in Molecular Biology (Vol. 887, pp. 149-165). (Methods in Molecular Biology; Vol. 887). Humana Press Inc.. https://doi.org/10.1007/978-1-61779-860-3_14

Generation of a bioengineered tooth by using a three-dimensional cell manipulation method (Organ Germ Method). / Oshima, Masamitsu; Ogawa, Miho; Yasukawa, Masato; Tsuji, Takashi.

Methods in Molecular Biology. Vol. 887 Humana Press Inc., 2012. p. 149-165 (Methods in Molecular Biology; Vol. 887).

Research output: Chapter in Book/Report/Conference proceedingChapter

Oshima, M, Ogawa, M, Yasukawa, M & Tsuji, T 2012, Generation of a bioengineered tooth by using a three-dimensional cell manipulation method (Organ Germ Method). in Methods in Molecular Biology. vol. 887, Methods in Molecular Biology, vol. 887, Humana Press Inc., pp. 149-165. https://doi.org/10.1007/978-1-61779-860-3_14
Oshima M, Ogawa M, Yasukawa M, Tsuji T. Generation of a bioengineered tooth by using a three-dimensional cell manipulation method (Organ Germ Method). In Methods in Molecular Biology. Vol. 887. Humana Press Inc. 2012. p. 149-165. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-61779-860-3_14
Oshima, Masamitsu ; Ogawa, Miho ; Yasukawa, Masato ; Tsuji, Takashi. / Generation of a bioengineered tooth by using a three-dimensional cell manipulation method (Organ Germ Method). Methods in Molecular Biology. Vol. 887 Humana Press Inc., 2012. pp. 149-165 (Methods in Molecular Biology).
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