Generation and characterization of MafA-Kusabira Orange mice

Wataru Nishimura, Hisashi Oishi, Nobuaki Funahashi, Toshiyoshi Fujiwara, Satoru Takahashi, Kazuki Yasuda

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

MafA and MafB are basic leucine zipper transcription factors expressed in mature pancreatic β- and α-cells, respectively. MafA is not only an insulin gene transcription factor but is also critical for the maturation and maintenance of β-cell function, whereas MafB is expressed in immature β-cells during development and in compromised β-cells in diabetes. In this study, we developed a mouse model to easily trace the promoter activity of MafA in β-cells as a tool for studying β-cell differentiation, maturation, regeneration and function using the expression of the fluorescent protein Kusabira Orange (KOr) driven by the BAC-mafA promoter. The expression of KOr was highly restricted to β-cells in the transgenic pancreas. By crossing MafA-KOr mice with MafBGFP/+ reporter mice, simultaneous monitoring of MafA and MafB expressions in the isolated islets was successfully performed. This system can be a useful tool for examining dynamic changes in the differentiation and function of pancreatic islets by visualizing the expressions of MafA and MafB.

Original languageEnglish
Pages (from-to)37-51
Number of pages15
JournalEndocrine journal
Volume62
Issue number1
DOIs
Publication statusPublished - 2015

Keywords

  • Kusabira Orange
  • MafA
  • MafB
  • Reporter
  • β-cells

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology

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  • Cite this

    Nishimura, W., Oishi, H., Funahashi, N., Fujiwara, T., Takahashi, S., & Yasuda, K. (2015). Generation and characterization of MafA-Kusabira Orange mice. Endocrine journal, 62(1), 37-51. https://doi.org/10.1507/endocrj.EJ14-0296