General flexible nature of the cytosolic regions of fungal transient receptor potential (TRP) channels, revealed by expression screening using GFP-fusion techniques

Makoto Ihara, Yoshitaka Takano, Atsuko Yamashita

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Transient receptor potential (TRP) channels are members of the voltage gated ion channel superfamily and display the unique characteristic of activation by diverse stimuli. We performed an expression analysis of fungal TRP channels, which possess relatively simple structures yet share the common functional characteristics with the other members, using a green fluorescent protein-based screening methodology. The analysis revealed that all the tested fungal TRP channels were severely digested in their cytosolic regions during expression, implying the common flexibility of this region, as observed in the recent structural analyses of the fungal member, TRPGz. These characteristics are likely to be important for their diverse functions.

Original languageEnglish
Pages (from-to)923-931
Number of pages9
JournalProtein Science
Volume23
Issue number7
DOIs
Publication statusPublished - Jul 2014

Keywords

  • GFP-fusion technique
  • Intrinsically disordered protein
  • TRP channels

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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