Gene transfer with liposomes to the intraocular tissues by different routes of administration

Ikuya Masuda, Toshihiko Matsuo, Tatsuji Yasuda, Nobuhiko Matsuo

Research output: Contribution to journalArticle

96 Citations (Scopus)

Abstract

Purpose. To determine whether a reporter gene carried by liposomes can be introduced into the ocular tissues in vivo by different routes of administration. Methods. Three different kinds of liposomes carrying plasmid DNA with beta-galactosidase gene were applied topically to the eye or were injected into the anterior chamber, subretinal space, and vitreous of adult Wistar rats. Gene expression was detected by enzymatic color reaction rising X-gal as a substrate in enucleated eyes 1 day, 1 week, and 1 month after topical application or injection. Results. Topical application could transfer the gene to retinal ganglion cells. Injection into the anterior chamber delivered the gene to the basal layer of the corneal epithelium, ciliary epithelium, stroma of the ciliary body and iris, and retinal ganglion cells. Injection into the vitreous or subretinal space resulted in the expression of the gene in the ciliary epithelium, stroma of the ciliary body and iris, retinal ganglion cells, and retinal pigment epithelial cells. Conclusions. Efficient and stable transfer of the functional gent could be achieved by liposomes in the cornea, iris, ciliary body, and retina of rats. Liposomes appear to be a promising vehicle for delivering therapeutic genes in vivo to mammalian intraocular tissues.

Original languageEnglish
Pages (from-to)1914-1920
Number of pages7
JournalInvestigative Ophthalmology and Visual Science
Volume37
Issue number9
Publication statusPublished - Aug 1996

Fingerprint

Liposomes
Ciliary Body
Retinal Ganglion Cells
Iris
Anterior Chamber
Genes
Injections
Epithelium
Gene Expression
Corneal Epithelium
Retinal Pigments
beta-Galactosidase
Reporter Genes
Cornea
Retina
Wistar Rats
Plasmids
Color
Epithelial Cells
DNA

Keywords

  • beta-galactosidase gene
  • cationic liposomes
  • eye
  • gene transfer
  • intraocular tissues

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Gene transfer with liposomes to the intraocular tissues by different routes of administration. / Masuda, Ikuya; Matsuo, Toshihiko; Yasuda, Tatsuji; Matsuo, Nobuhiko.

In: Investigative Ophthalmology and Visual Science, Vol. 37, No. 9, 08.1996, p. 1914-1920.

Research output: Contribution to journalArticle

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N2 - Purpose. To determine whether a reporter gene carried by liposomes can be introduced into the ocular tissues in vivo by different routes of administration. Methods. Three different kinds of liposomes carrying plasmid DNA with beta-galactosidase gene were applied topically to the eye or were injected into the anterior chamber, subretinal space, and vitreous of adult Wistar rats. Gene expression was detected by enzymatic color reaction rising X-gal as a substrate in enucleated eyes 1 day, 1 week, and 1 month after topical application or injection. Results. Topical application could transfer the gene to retinal ganglion cells. Injection into the anterior chamber delivered the gene to the basal layer of the corneal epithelium, ciliary epithelium, stroma of the ciliary body and iris, and retinal ganglion cells. Injection into the vitreous or subretinal space resulted in the expression of the gene in the ciliary epithelium, stroma of the ciliary body and iris, retinal ganglion cells, and retinal pigment epithelial cells. Conclusions. Efficient and stable transfer of the functional gent could be achieved by liposomes in the cornea, iris, ciliary body, and retina of rats. Liposomes appear to be a promising vehicle for delivering therapeutic genes in vivo to mammalian intraocular tissues.

AB - Purpose. To determine whether a reporter gene carried by liposomes can be introduced into the ocular tissues in vivo by different routes of administration. Methods. Three different kinds of liposomes carrying plasmid DNA with beta-galactosidase gene were applied topically to the eye or were injected into the anterior chamber, subretinal space, and vitreous of adult Wistar rats. Gene expression was detected by enzymatic color reaction rising X-gal as a substrate in enucleated eyes 1 day, 1 week, and 1 month after topical application or injection. Results. Topical application could transfer the gene to retinal ganglion cells. Injection into the anterior chamber delivered the gene to the basal layer of the corneal epithelium, ciliary epithelium, stroma of the ciliary body and iris, and retinal ganglion cells. Injection into the vitreous or subretinal space resulted in the expression of the gene in the ciliary epithelium, stroma of the ciliary body and iris, retinal ganglion cells, and retinal pigment epithelial cells. Conclusions. Efficient and stable transfer of the functional gent could be achieved by liposomes in the cornea, iris, ciliary body, and retina of rats. Liposomes appear to be a promising vehicle for delivering therapeutic genes in vivo to mammalian intraocular tissues.

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