Gene silencing using a heat-inducible RNAi system in Arabidopsis

Frédéric Masclaux, Martine Charpenteau, Taku Takahashi, Rafael Pont-Lezica, Jean Philippe Galaud

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Controlling gene expression during plant development is an efficient tool to explore gene function. In this paper, we describe a gene expression system driven by a heat-shock gene promoter (HSP18.2), to trigger the expression of an intron-containing inverted-repeat. RNA interference became a powerful way for gene functional analysis by reverse genetic approaches. However, constitutive gene silencing cannot be used with genes involved in fundamental processes such as embryo viability. Inducible promoters provide an alternative approach for temporal and spatial gene expression control and we described here a new system, complementary to those using chemical gene inducers. To evaluate the efficiency of this system, RNA corresponding to the phytoene desaturase gene of Arabidopsis thaliana was used as a reporter gene in transgenic plants and a comparative study was performed using either the CaMV35S constitutive promoter or the HSP18.2 inducible promoter.

Original languageEnglish
Pages (from-to)364-369
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume321
Issue number2
DOIs
Publication statusPublished - Aug 20 2004

Fingerprint

Gene Silencing
RNA Interference
Arabidopsis
Hot Temperature
Genes
Gene expression
Gene Expression
Reverse Genetics
Plant Development
Genetically Modified Plants
RNA
Reporter Genes
Introns
Functional analysis
Shock
Embryonic Structures

Keywords

  • Arabidopsis
  • Conditional gene expression
  • Heat-shock
  • Phytoene desaturase
  • RNA interference

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Gene silencing using a heat-inducible RNAi system in Arabidopsis. / Masclaux, Frédéric; Charpenteau, Martine; Takahashi, Taku; Pont-Lezica, Rafael; Galaud, Jean Philippe.

In: Biochemical and Biophysical Research Communications, Vol. 321, No. 2, 20.08.2004, p. 364-369.

Research output: Contribution to journalArticle

Masclaux, Frédéric ; Charpenteau, Martine ; Takahashi, Taku ; Pont-Lezica, Rafael ; Galaud, Jean Philippe. / Gene silencing using a heat-inducible RNAi system in Arabidopsis. In: Biochemical and Biophysical Research Communications. 2004 ; Vol. 321, No. 2. pp. 364-369.
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