Abstract
Controlling gene expression during plant development is an efficient tool to explore gene function. In this paper, we describe a gene expression system driven by a heat-shock gene promoter (HSP18.2), to trigger the expression of an intron-containing inverted-repeat. RNA interference became a powerful way for gene functional analysis by reverse genetic approaches. However, constitutive gene silencing cannot be used with genes involved in fundamental processes such as embryo viability. Inducible promoters provide an alternative approach for temporal and spatial gene expression control and we described here a new system, complementary to those using chemical gene inducers. To evaluate the efficiency of this system, RNA corresponding to the phytoene desaturase gene of Arabidopsis thaliana was used as a reporter gene in transgenic plants and a comparative study was performed using either the CaMV35S constitutive promoter or the HSP18.2 inducible promoter.
| Original language | English |
|---|---|
| Pages (from-to) | 364-369 |
| Number of pages | 6 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 321 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Aug 20 2004 |
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Keywords
- Arabidopsis
- Conditional gene expression
- Heat-shock
- Phytoene desaturase
- RNA interference
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
Gene silencing using a heat-inducible RNAi system in Arabidopsis. / Masclaux, Frédéric; Charpenteau, Martine; Takahashi, Taku; Pont-Lezica, Rafael; Galaud, Jean Philippe.
In: Biochemical and Biophysical Research Communications, Vol. 321, No. 2, 20.08.2004, p. 364-369.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Gene silencing using a heat-inducible RNAi system in Arabidopsis
AU - Masclaux, Frédéric
AU - Charpenteau, Martine
AU - Takahashi, Taku
AU - Pont-Lezica, Rafael
AU - Galaud, Jean Philippe
PY - 2004/8/20
Y1 - 2004/8/20
N2 - Controlling gene expression during plant development is an efficient tool to explore gene function. In this paper, we describe a gene expression system driven by a heat-shock gene promoter (HSP18.2), to trigger the expression of an intron-containing inverted-repeat. RNA interference became a powerful way for gene functional analysis by reverse genetic approaches. However, constitutive gene silencing cannot be used with genes involved in fundamental processes such as embryo viability. Inducible promoters provide an alternative approach for temporal and spatial gene expression control and we described here a new system, complementary to those using chemical gene inducers. To evaluate the efficiency of this system, RNA corresponding to the phytoene desaturase gene of Arabidopsis thaliana was used as a reporter gene in transgenic plants and a comparative study was performed using either the CaMV35S constitutive promoter or the HSP18.2 inducible promoter.
AB - Controlling gene expression during plant development is an efficient tool to explore gene function. In this paper, we describe a gene expression system driven by a heat-shock gene promoter (HSP18.2), to trigger the expression of an intron-containing inverted-repeat. RNA interference became a powerful way for gene functional analysis by reverse genetic approaches. However, constitutive gene silencing cannot be used with genes involved in fundamental processes such as embryo viability. Inducible promoters provide an alternative approach for temporal and spatial gene expression control and we described here a new system, complementary to those using chemical gene inducers. To evaluate the efficiency of this system, RNA corresponding to the phytoene desaturase gene of Arabidopsis thaliana was used as a reporter gene in transgenic plants and a comparative study was performed using either the CaMV35S constitutive promoter or the HSP18.2 inducible promoter.
KW - Arabidopsis
KW - Conditional gene expression
KW - Heat-shock
KW - Phytoene desaturase
KW - RNA interference
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UR - http://www.scopus.com/inward/citedby.url?scp=3242809747&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2004.06.154
DO - 10.1016/j.bbrc.2004.06.154
M3 - Article
VL - 321
SP - 364
EP - 369
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 2
ER -