Gene profiling in human periodontal ligament fibroblasts by subtractive hybridization

Tadashi Yamamoto, F. Myokai, F. Nishimura, T. Ohira, N. Shiomi, Keisuke Yamashiro, H. Arai, Y. Murayama, Shogo Takashiba

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Genes expressed by human periodontal ligament fibroblasts (HPFs) are likely to be associated with specific functions of the ligament. The aim of this study is to profile genes expressed highly by HPFs. A library (6 × 10 3 pfu) was constructed, followed by subtraction of HPF cDNAs with human gingival fibroblast (HGF) cDNAs. Reverse-dot hybridization revealed that 33 clones expressed higher levels of specific mRNAs in HPFs than in HGFs. These were mRNAs for known genes, including several associated with maturation and differentiation of cells. None had been reported in PFs. One clone, PDL-29, identified as a COX assembly factor, showed much stronger mRNA expression in HPFs than in HGFs in culture. In rat periodontium, however, PDL-29 mRNA expression was similar in PFs and GFs. These results suggest that HPFs express many previously unreported genes associated with maturation and differentiation, but expression can differ in vitro and in vivo.

Original languageEnglish
Pages (from-to)641-645
Number of pages5
JournalJournal of Dental Research
Volume82
Issue number8
Publication statusPublished - Aug 2003

Fingerprint

Periodontal Ligament
Fibroblasts
Genes
Messenger RNA
Complementary DNA
Clone Cells
Periodontium
Subtractive Hybridization Techniques
Ligaments
Libraries
Cell Differentiation

Keywords

  • Gene profiling
  • In situ hybridization
  • Periodontal ligament fibroblasts
  • Subtractive hybridization

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Gene profiling in human periodontal ligament fibroblasts by subtractive hybridization. / Yamamoto, Tadashi; Myokai, F.; Nishimura, F.; Ohira, T.; Shiomi, N.; Yamashiro, Keisuke; Arai, H.; Murayama, Y.; Takashiba, Shogo.

In: Journal of Dental Research, Vol. 82, No. 8, 08.2003, p. 641-645.

Research output: Contribution to journalArticle

Yamamoto, T, Myokai, F, Nishimura, F, Ohira, T, Shiomi, N, Yamashiro, K, Arai, H, Murayama, Y & Takashiba, S 2003, 'Gene profiling in human periodontal ligament fibroblasts by subtractive hybridization', Journal of Dental Research, vol. 82, no. 8, pp. 641-645.
Yamamoto, Tadashi ; Myokai, F. ; Nishimura, F. ; Ohira, T. ; Shiomi, N. ; Yamashiro, Keisuke ; Arai, H. ; Murayama, Y. ; Takashiba, Shogo. / Gene profiling in human periodontal ligament fibroblasts by subtractive hybridization. In: Journal of Dental Research. 2003 ; Vol. 82, No. 8. pp. 641-645.
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