Gene limiting cadmium accumulation in rice

Daisei Ueno, Naoki Yamaji, Izumi Kono, Chao Feng Huang, Tsuyu Ando, Masahiro Yano, Jian Feng Ma

Research output: Contribution to journalArticlepeer-review

570 Citations (Scopus)


Intake of toxic cadmium (Cd) from rice caused Itai-itai disease in the past and it is still a threat for human health. Therefore, control of the accumulation of Cd from soil is an important food-safety issue, but the molecular mechanism for the control is unknown. Herein, we report a gene (OsHMA3) responsible for low Cd accumulation in rice that was isolated from a mapping population derived from a cross between a high and low Cd-accumulating cultivar. The gene encodes a transporter belonging to the P1B-type ATPase family, but shares low similarity with other members. Heterologous expression in yeast showed that the transporter from the low-Cd cultivar is functional, but the transporter from the high-Cd cultivar had lost its function, probably because of the single amino acid mutation. The transporter is mainly expressed in the tonoplast of root cells at a similar level in both the low and high Cd-accumulating cultivars. Overexpression of the functional gene from the low Cd-accumulating cultivar selectively decreased accumulation of Cd, but not other micronutrients in the grain. Our results indicated that OsHMA3 from the low Cd-accumulating cultivar limits translocation of Cd from the roots to the above-ground tissues by selectively sequestrating Cd into the root vacuoles.

Original languageEnglish
Pages (from-to)16500-16505
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number38
Publication statusPublished - Sept 21 2010

ASJC Scopus subject areas

  • General


Dive into the research topics of 'Gene limiting cadmium accumulation in rice'. Together they form a unique fingerprint.

Cite this