Gene limiting cadmium accumulation in rice

Daisei Ueno, Naoki Yamaji, Izumi Kono, Chao Feng Huang, Tsuyu Ando, Masahiro Yano, Jian Feng Ma

Research output: Contribution to journalArticle

309 Citations (Scopus)

Abstract

Intake of toxic cadmium (Cd) from rice caused Itai-itai disease in the past and it is still a threat for human health. Therefore, control of the accumulation of Cd from soil is an important food-safety issue, but the molecular mechanism for the control is unknown. Herein, we report a gene (OsHMA3) responsible for low Cd accumulation in rice that was isolated from a mapping population derived from a cross between a high and low Cd-accumulating cultivar. The gene encodes a transporter belonging to the P1B-type ATPase family, but shares low similarity with other members. Heterologous expression in yeast showed that the transporter from the low-Cd cultivar is functional, but the transporter from the high-Cd cultivar had lost its function, probably because of the single amino acid mutation. The transporter is mainly expressed in the tonoplast of root cells at a similar level in both the low and high Cd-accumulating cultivars. Overexpression of the functional gene from the low Cd-accumulating cultivar selectively decreased accumulation of Cd, but not other micronutrients in the grain. Our results indicated that OsHMA3 from the low Cd-accumulating cultivar limits translocation of Cd from the roots to the above-ground tissues by selectively sequestrating Cd into the root vacuoles.

Original languageEnglish
Pages (from-to)16500-16505
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume107
Issue number38
DOIs
Publication statusPublished - Sep 21 2010

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Cadmium
Genes
Oryza
Cadmium Poisoning
Food Safety
Micronutrients
Poisons
Vacuoles
Adenosine Triphosphatases
Soil
Yeasts
Amino Acids
Mutation

ASJC Scopus subject areas

  • General
  • Medicine(all)

Cite this

Gene limiting cadmium accumulation in rice. / Ueno, Daisei; Yamaji, Naoki; Kono, Izumi; Huang, Chao Feng; Ando, Tsuyu; Yano, Masahiro; Ma, Jian Feng.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, No. 38, 21.09.2010, p. 16500-16505.

Research output: Contribution to journalArticle

Ueno, Daisei ; Yamaji, Naoki ; Kono, Izumi ; Huang, Chao Feng ; Ando, Tsuyu ; Yano, Masahiro ; Ma, Jian Feng. / Gene limiting cadmium accumulation in rice. In: Proceedings of the National Academy of Sciences of the United States of America. 2010 ; Vol. 107, No. 38. pp. 16500-16505.
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