Gene expression profiling of the anti-glioma effect of Cilengitide

Manabu Onishi, Kazuhiko Kurozumi, Tomotsugu Ichikawa, Hiroyuki Michiue, Kentaro Fujii, Joji Ishida, Yosuke Shimazu, E. Antonio Chiocca, Balveen Kaur, Isao Date

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Cilengitide (EMD121974), an inhibitor of the adhesive function of integrins, demonstrated preclinical efficacy against malignant glioma. It is speculated that cilengitide can inhibit tumor growth, invasion, and angiogenesis. However, the effects of cilengitide on these processes have not been sufficiently examined. In this study, we investigated the anti-glioma effect of cilengitide using DNA microarray analysis. U87ΔEGFR cells (human malignant glioma cell line) were used for this experiment. The cells were harvested after 16 h of cilengitide treatment, and mRNA was extracted. Gene expression and pathway analyses were performed using a DNA microarray (CodeLink™ Human Whole Genome Bioarray). The expression of 264 genes was changed with cilengitide treatment. The expression of 214 genes was up-regulated by more than 4-fold and the expression of 50 genes was down-regulated by more than 4-fold compared to the controls. In pathway analysis, "apoptotic cleavage of cellular proteins" and "TNF receptor signaling pathway" were over-represented. Apoptoticassociated genes such as caspase 8 were up-regulated. Gene expression profiling revealed more detailed mechanism of the anti-glioma effect of cilengitide. Genes associated with apoptosis were over-represented following cilengitide treatment.

Original languageEnglish
Article number160
JournalSpringerPlus
Volume2
Issue number1
DOIs
Publication statusPublished - 2013

Keywords

  • Apoptosis
  • Cilengitide
  • Gene expression profiling
  • Glioma
  • Integrin

ASJC Scopus subject areas

  • General

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