Gene expression profile of mouse masseter muscle after repetitive electrical stimulation

Tsuyoshi Ono, Kenji Maekawa, Wataru Sonoyama, Shunji Kojima, Tsutomu Tanaka, Glenn T. Clark, Takuo Kuboki

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Purpose: To examine gene expression profile changes in the mouse masseter muscle tissue after repetitive electrical stimulation by using a DNA microarray technique. Methods: Nine male ICR mice aged 10 weeks were used. Each anesthetized mouse was secured on a platform in a supine position and the masseter muscle tissues on both sides were exposed. Bipolar electrodes were set on the right masseteric fascia to electrically stimulate the masseter muscle (8 V, 10 Hz, 20 ms) for 30 min. After cessation of stimulation bilateral masseter muscle tissues were sampled at 0 h (n = 3), 1 h (n = 3), 2 h (n = 3). Total RNA was isolated from the homogenized muscle tissues and purified mRNA samples (50 μg) were processed and hybridized with microarray slides. Probe arrays were then scanned and analyzed to calculate the signal density. Gene expression profiles were compared at each time point between the right (stimulation side) and left (control side) masseter. When the gene expression levels were different more than 2-fold, the difference was regarded as positive. Results: Of the 6400 genes assessed, 1733 genes were up-regulated and 515 genes were down-regulated in the stimulation side at least once during the experimental time course. These up- or down-regulated genes were associated with autoimmune/inflammatory disease (28/114), cardiovascular disease (17/61), neuroscience (12/50), apoptosis (27/93), diabetes/obesity (9/28), signal transduction (66/250) and others. 28 genes were up-regulated and 25 genes were down-regulated at all time points. Conclusions: Dramatic gene expression changes were induced by the repetitive electrical muscle stimulation in mouse masseter.

Original languageEnglish
Pages (from-to)36-41
Number of pages6
JournalJournal of Prosthodontic Research
Volume54
Issue number1
DOIs
Publication statusPublished - Jan 2010

Fingerprint

Masseter Muscle
Transcriptome
Electric Stimulation
Muscles
Genes
Gene Expression
Inbred ICR Mouse
Supine Position
Fascia
Neurosciences
Oligonucleotide Array Sequence Analysis
Autoimmune Diseases
Signal Transduction
Electrodes
Cardiovascular Diseases
Obesity
RNA
Apoptosis
Messenger RNA

Keywords

  • Electrical stimulation
  • Gene expression
  • Masseter muscle
  • Microarray
  • Mouse

ASJC Scopus subject areas

  • Dentistry (miscellaneous)
  • Oral Surgery

Cite this

Gene expression profile of mouse masseter muscle after repetitive electrical stimulation. / Ono, Tsuyoshi; Maekawa, Kenji; Sonoyama, Wataru; Kojima, Shunji; Tanaka, Tsutomu; Clark, Glenn T.; Kuboki, Takuo.

In: Journal of Prosthodontic Research, Vol. 54, No. 1, 01.2010, p. 36-41.

Research output: Contribution to journalArticle

Ono, Tsuyoshi ; Maekawa, Kenji ; Sonoyama, Wataru ; Kojima, Shunji ; Tanaka, Tsutomu ; Clark, Glenn T. ; Kuboki, Takuo. / Gene expression profile of mouse masseter muscle after repetitive electrical stimulation. In: Journal of Prosthodontic Research. 2010 ; Vol. 54, No. 1. pp. 36-41.
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