Gene expression of rice seeds surviving 13- and 20-month exposure to space environment

Manabu Sugimoto, Youko Oono, Yoshihiro Kawahara, Oleg Gusev, Masahiko Maekawa, Takashi Matsumoto, Margarita Levinskikh, Vladimir Sychev, Natalia Novikova, Anatoly Grigoriev

Research output: Contribution to journalArticle

Abstract

Rice seeds were exposed outside of the international space station to assess the risk of space environment exposure on gene expression associated with seed germination. The germination percentages of the space-stored and ground-stored seeds exposed for 13 months were 48 and 96% respectively. Those for 20 months were 7 and 76%, respectively. Germination was defined 3 days after imbibition, except for the space-stored seeds exposed for 20 months, which germinated 5 days after imbibition. Subsequent RNA-seq analyses of the dry seeds, germinated seeds, and roots and shoots of seedlings revealed that the mutation rates of mRNA sequences were not significantly different between space-stored and ground-stored samples exposed for 13 months and 20 months. In all, 4 and 16 transcripts of glycolysis-related genes were increased in the germinated seeds after 13-month and 20-month exposure, respectively. Also, 2 and 39 transcripts of long-lived mRNA required for germination were decreased more than 2-fold in the dry seeds after 13-month and 20-month exposure, respectively. These results suggest that damage to long-lived mRNA in seeds by a space environment delays and reduces germination.

Original languageEnglish
Pages (from-to)10-17
Number of pages8
JournalLife Sciences in Space Research
Volume11
DOIs
Publication statusPublished - Nov 2016
Externally publishedYes

Fingerprint

aerospace environments
gene expression
rice
seeds
Seeds
germination
seed
Gene Expression
Germination
imbibition
Messenger RNA
glycolysis
exposure
Oryza
International Space Station
Glycolysis
Mutation Rate
mutations
Seedlings
genes

Keywords

  • Journal Article

Cite this

Gene expression of rice seeds surviving 13- and 20-month exposure to space environment. / Sugimoto, Manabu; Oono, Youko; Kawahara, Yoshihiro; Gusev, Oleg; Maekawa, Masahiko; Matsumoto, Takashi; Levinskikh, Margarita; Sychev, Vladimir; Novikova, Natalia; Grigoriev, Anatoly.

In: Life Sciences in Space Research, Vol. 11, 11.2016, p. 10-17.

Research output: Contribution to journalArticle

Sugimoto, M, Oono, Y, Kawahara, Y, Gusev, O, Maekawa, M, Matsumoto, T, Levinskikh, M, Sychev, V, Novikova, N & Grigoriev, A 2016, 'Gene expression of rice seeds surviving 13- and 20-month exposure to space environment', Life Sciences in Space Research, vol. 11, pp. 10-17. https://doi.org/10.1016/j.lssr.2016.10.001
Sugimoto, Manabu ; Oono, Youko ; Kawahara, Yoshihiro ; Gusev, Oleg ; Maekawa, Masahiko ; Matsumoto, Takashi ; Levinskikh, Margarita ; Sychev, Vladimir ; Novikova, Natalia ; Grigoriev, Anatoly. / Gene expression of rice seeds surviving 13- and 20-month exposure to space environment. In: Life Sciences in Space Research. 2016 ; Vol. 11. pp. 10-17.
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abstract = "Rice seeds were exposed outside of the international space station to assess the risk of space environment exposure on gene expression associated with seed germination. The germination percentages of the space-stored and ground-stored seeds exposed for 13 months were 48 and 96{\%} respectively. Those for 20 months were 7 and 76{\%}, respectively. Germination was defined 3 days after imbibition, except for the space-stored seeds exposed for 20 months, which germinated 5 days after imbibition. Subsequent RNA-seq analyses of the dry seeds, germinated seeds, and roots and shoots of seedlings revealed that the mutation rates of mRNA sequences were not significantly different between space-stored and ground-stored samples exposed for 13 months and 20 months. In all, 4 and 16 transcripts of glycolysis-related genes were increased in the germinated seeds after 13-month and 20-month exposure, respectively. Also, 2 and 39 transcripts of long-lived mRNA required for germination were decreased more than 2-fold in the dry seeds after 13-month and 20-month exposure, respectively. These results suggest that damage to long-lived mRNA in seeds by a space environment delays and reduces germination.",
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AU - Matsumoto, Takashi

AU - Levinskikh, Margarita

AU - Sychev, Vladimir

AU - Novikova, Natalia

AU - Grigoriev, Anatoly

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N2 - Rice seeds were exposed outside of the international space station to assess the risk of space environment exposure on gene expression associated with seed germination. The germination percentages of the space-stored and ground-stored seeds exposed for 13 months were 48 and 96% respectively. Those for 20 months were 7 and 76%, respectively. Germination was defined 3 days after imbibition, except for the space-stored seeds exposed for 20 months, which germinated 5 days after imbibition. Subsequent RNA-seq analyses of the dry seeds, germinated seeds, and roots and shoots of seedlings revealed that the mutation rates of mRNA sequences were not significantly different between space-stored and ground-stored samples exposed for 13 months and 20 months. In all, 4 and 16 transcripts of glycolysis-related genes were increased in the germinated seeds after 13-month and 20-month exposure, respectively. Also, 2 and 39 transcripts of long-lived mRNA required for germination were decreased more than 2-fold in the dry seeds after 13-month and 20-month exposure, respectively. These results suggest that damage to long-lived mRNA in seeds by a space environment delays and reduces germination.

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