Abstract
Rice seeds were exposed outside of the international space station to assess the risk of space environment exposure on gene expression associated with seed germination. The germination percentages of the space-stored and ground-stored seeds exposed for 13 months were 48 and 96% respectively. Those for 20 months were 7 and 76%, respectively. Germination was defined 3 days after imbibition, except for the space-stored seeds exposed for 20 months, which germinated 5 days after imbibition. Subsequent RNA-seq analyses of the dry seeds, germinated seeds, and roots and shoots of seedlings revealed that the mutation rates of mRNA sequences were not significantly different between space-stored and ground-stored samples exposed for 13 months and 20 months. In all, 4 and 16 transcripts of glycolysis-related genes were increased in the germinated seeds after 13-month and 20-month exposure, respectively. Also, 2 and 39 transcripts of long-lived mRNA required for germination were decreased more than 2-fold in the dry seeds after 13-month and 20-month exposure, respectively. These results suggest that damage to long-lived mRNA in seeds by a space environment delays and reduces germination.
| Original language | English |
|---|---|
| Pages (from-to) | 10-17 |
| Number of pages | 8 |
| Journal | Life Sciences in Space Research |
| Volume | 11 |
| DOIs | |
| Publication status | Published - Nov 2016 |
| Externally published | Yes |
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- Journal Article
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Gene expression of rice seeds surviving 13- and 20-month exposure to space environment. / Sugimoto, Manabu; Oono, Youko; Kawahara, Yoshihiro; Gusev, Oleg; Maekawa, Masahiko; Matsumoto, Takashi; Levinskikh, Margarita; Sychev, Vladimir; Novikova, Natalia; Grigoriev, Anatoly.
In: Life Sciences in Space Research, Vol. 11, 11.2016, p. 10-17.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Gene expression of rice seeds surviving 13- and 20-month exposure to space environment
AU - Sugimoto, Manabu
AU - Oono, Youko
AU - Kawahara, Yoshihiro
AU - Gusev, Oleg
AU - Maekawa, Masahiko
AU - Matsumoto, Takashi
AU - Levinskikh, Margarita
AU - Sychev, Vladimir
AU - Novikova, Natalia
AU - Grigoriev, Anatoly
N1 - Copyright © 2016 The Committee on Space Research (COSPAR). Published by Elsevier Ltd. All rights reserved.
PY - 2016/11
Y1 - 2016/11
N2 - Rice seeds were exposed outside of the international space station to assess the risk of space environment exposure on gene expression associated with seed germination. The germination percentages of the space-stored and ground-stored seeds exposed for 13 months were 48 and 96% respectively. Those for 20 months were 7 and 76%, respectively. Germination was defined 3 days after imbibition, except for the space-stored seeds exposed for 20 months, which germinated 5 days after imbibition. Subsequent RNA-seq analyses of the dry seeds, germinated seeds, and roots and shoots of seedlings revealed that the mutation rates of mRNA sequences were not significantly different between space-stored and ground-stored samples exposed for 13 months and 20 months. In all, 4 and 16 transcripts of glycolysis-related genes were increased in the germinated seeds after 13-month and 20-month exposure, respectively. Also, 2 and 39 transcripts of long-lived mRNA required for germination were decreased more than 2-fold in the dry seeds after 13-month and 20-month exposure, respectively. These results suggest that damage to long-lived mRNA in seeds by a space environment delays and reduces germination.
AB - Rice seeds were exposed outside of the international space station to assess the risk of space environment exposure on gene expression associated with seed germination. The germination percentages of the space-stored and ground-stored seeds exposed for 13 months were 48 and 96% respectively. Those for 20 months were 7 and 76%, respectively. Germination was defined 3 days after imbibition, except for the space-stored seeds exposed for 20 months, which germinated 5 days after imbibition. Subsequent RNA-seq analyses of the dry seeds, germinated seeds, and roots and shoots of seedlings revealed that the mutation rates of mRNA sequences were not significantly different between space-stored and ground-stored samples exposed for 13 months and 20 months. In all, 4 and 16 transcripts of glycolysis-related genes were increased in the germinated seeds after 13-month and 20-month exposure, respectively. Also, 2 and 39 transcripts of long-lived mRNA required for germination were decreased more than 2-fold in the dry seeds after 13-month and 20-month exposure, respectively. These results suggest that damage to long-lived mRNA in seeds by a space environment delays and reduces germination.
KW - Journal Article
U2 - 10.1016/j.lssr.2016.10.001
DO - 10.1016/j.lssr.2016.10.001
M3 - Article
C2 - 27993188
VL - 11
SP - 10
EP - 17
JO - Life Sciences in Space Research
JF - Life Sciences in Space Research
SN - 2214-5524
ER -