Gene expression of liver-specific proteins in hepatocyte spheroids in primary culture.

T. Tamura, N. Koide, H. Hada, Hidenori Shiraha, T. Tsuji

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Adult rat hepatocytes assemble to form multicellular spheroids under non-adherent environments such as immobilized chondroitin sulfate-proteoglycan in primary culture. Previously, we demonstrated that hepatocyte spheroids exhibited various differentiated structures as observed in the liver tissue. It was also shown that hepatocyte growth was highly suppressed and several differentiated functions, including albumin production and gluconeogenesis, were well preserved in spheroids. To investigate the differentiated functions of cultured hepatocytes in relation to cell morphology, we compared the expression of the albumin and transferrin genes in spheroids with those in monolayers by Northern blot analysis. Production of these proteins in the culture medium was simultaneously examined by ELISA. Gene expression and protein production of both albumin and transferrin were better preserved in spheroids. We also examined changes in the expression of liver-specific genes in response to IL-6. Reduced mRNA levels of both albumin and transferrin was only found in spheroids and no change was observed in monolayers. These results suggest that the regulation of tissue-specific gene expression is better preserved in spheroids, in which hepatocytes are in close contact with each other.

Original languageEnglish
Pages (from-to)161-167
Number of pages7
JournalActa Medica Okayama
Volume49
Issue number3
Publication statusPublished - Jun 1995

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Gene expression
Liver
Hepatocytes
Albumins
Gene Expression
Transferrin
Proteins
Monolayers
Genes
Cellular Spheroids
Tissue
Chondroitin Sulfate Proteoglycans
Gluconeogenesis
Northern Blotting
Culture Media
Rats
Interleukin-6
Enzyme-Linked Immunosorbent Assay
Messenger RNA
Growth

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Gene expression of liver-specific proteins in hepatocyte spheroids in primary culture. / Tamura, T.; Koide, N.; Hada, H.; Shiraha, Hidenori; Tsuji, T.

In: Acta Medica Okayama, Vol. 49, No. 3, 06.1995, p. 161-167.

Research output: Contribution to journalArticle

Tamura, T, Koide, N, Hada, H, Shiraha, H & Tsuji, T 1995, 'Gene expression of liver-specific proteins in hepatocyte spheroids in primary culture.', Acta Medica Okayama, vol. 49, no. 3, pp. 161-167.
Tamura, T. ; Koide, N. ; Hada, H. ; Shiraha, Hidenori ; Tsuji, T. / Gene expression of liver-specific proteins in hepatocyte spheroids in primary culture. In: Acta Medica Okayama. 1995 ; Vol. 49, No. 3. pp. 161-167.
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