Abstract
The heat shock protein gene, hsp70, was cloned and sequenced from the diamondback moth, Plutella xylostella (L.). cDNA sequence analysis revealed a 1,992 bp-long open reading frame, which encodes a protein with a calculated molecular weight of 72.2 kDa. Results of Southern blot analysis suggested the presence of multiple copies of the hsp70 family in the genome. Increased expression of hsp70 was observed in fourth instar larvae exposed to 42°C. No significant up-regulation of hsp70 expression was observed when fourth instar larvae were treated with sub-lethal doses of insecticides, chlorfenapyr, permethrin, and acephate. These results suggest that hsp70 cloned in the present study is less sensitive to insecticides.
| Original language | English |
|---|---|
| Pages (from-to) | 241-247 |
| Number of pages | 7 |
| Journal | Applied Entomology and Zoology |
| Volume | 43 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2008 |
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Keywords
- Acephate
- Chlorfenapyr
- Heat shock protein
- Permethrin
- Plutella xylostella
ASJC Scopus subject areas
- Insect Science
Cite this
Gene expression of hsp70 of the diamondback moth, Plutella xylostella (Lepidoptera : Yponomeutidae), in response to heat shock and insecticides. / Sonoda, Shoji; Tsumuki, Hisaaki.
In: Applied Entomology and Zoology, Vol. 43, No. 2, 2008, p. 241-247.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Gene expression of hsp70 of the diamondback moth, Plutella xylostella (Lepidoptera
T2 - Yponomeutidae), in response to heat shock and insecticides
AU - Sonoda, Shoji
AU - Tsumuki, Hisaaki
PY - 2008
Y1 - 2008
N2 - The heat shock protein gene, hsp70, was cloned and sequenced from the diamondback moth, Plutella xylostella (L.). cDNA sequence analysis revealed a 1,992 bp-long open reading frame, which encodes a protein with a calculated molecular weight of 72.2 kDa. Results of Southern blot analysis suggested the presence of multiple copies of the hsp70 family in the genome. Increased expression of hsp70 was observed in fourth instar larvae exposed to 42°C. No significant up-regulation of hsp70 expression was observed when fourth instar larvae were treated with sub-lethal doses of insecticides, chlorfenapyr, permethrin, and acephate. These results suggest that hsp70 cloned in the present study is less sensitive to insecticides.
AB - The heat shock protein gene, hsp70, was cloned and sequenced from the diamondback moth, Plutella xylostella (L.). cDNA sequence analysis revealed a 1,992 bp-long open reading frame, which encodes a protein with a calculated molecular weight of 72.2 kDa. Results of Southern blot analysis suggested the presence of multiple copies of the hsp70 family in the genome. Increased expression of hsp70 was observed in fourth instar larvae exposed to 42°C. No significant up-regulation of hsp70 expression was observed when fourth instar larvae were treated with sub-lethal doses of insecticides, chlorfenapyr, permethrin, and acephate. These results suggest that hsp70 cloned in the present study is less sensitive to insecticides.
KW - Acephate
KW - Chlorfenapyr
KW - Heat shock protein
KW - Permethrin
KW - Plutella xylostella
UR - http://www.scopus.com/inward/record.url?scp=50449094675&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=50449094675&partnerID=8YFLogxK
U2 - 10.1303/aez.2008.241
DO - 10.1303/aez.2008.241
M3 - Article
AN - SCOPUS:50449094675
VL - 43
SP - 241
EP - 247
JO - Applied Entomology and Zoology
JF - Applied Entomology and Zoology
SN - 0003-6862
IS - 2
ER -