Gene expression of hsp70 of the diamondback moth, Plutella xylostella (Lepidoptera: Yponomeutidae), in response to heat shock and insecticides

Shoji Sonoda, Hisaaki Tsumuki

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The heat shock protein gene, hsp70, was cloned and sequenced from the diamondback moth, Plutella xylostella (L.). cDNA sequence analysis revealed a 1,992 bp-long open reading frame, which encodes a protein with a calculated molecular weight of 72.2 kDa. Results of Southern blot analysis suggested the presence of multiple copies of the hsp70 family in the genome. Increased expression of hsp70 was observed in fourth instar larvae exposed to 42°C. No significant up-regulation of hsp70 expression was observed when fourth instar larvae were treated with sub-lethal doses of insecticides, chlorfenapyr, permethrin, and acephate. These results suggest that hsp70 cloned in the present study is less sensitive to insecticides.

Original languageEnglish
Pages (from-to)241-247
Number of pages7
JournalApplied Entomology and Zoology
Volume43
Issue number2
DOIs
Publication statusPublished - 2008

Fingerprint

Yponomeutidae
Plutella xylostella
heat stress
insecticides
instars
Lepidoptera
chlorfenapyr
acephate
gene expression
larvae
permethrin
lethal dose
heat shock proteins
Southern blotting
open reading frames
sequence analysis
molecular weight
genome
genes
proteins

Keywords

  • Acephate
  • Chlorfenapyr
  • Heat shock protein
  • Permethrin
  • Plutella xylostella

ASJC Scopus subject areas

  • Insect Science

Cite this

Gene expression of hsp70 of the diamondback moth, Plutella xylostella (Lepidoptera : Yponomeutidae), in response to heat shock and insecticides. / Sonoda, Shoji; Tsumuki, Hisaaki.

In: Applied Entomology and Zoology, Vol. 43, No. 2, 2008, p. 241-247.

Research output: Contribution to journalArticle

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