Gene Expression of Arginine Vasotocin in Ovarian and Uterine Tissues of the Chicken

Noboru Saito, R. Grossmann

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

The hypothalamus is the classic site of synthesis of arginine vasotocin as neurohypophyseal hormone in the chicken. However, high concentrations of arginine vasotocin were also measured in ovarian tissues by radioimmunoassay. At first, we observed specific positive signal of rnRNA encoding AVT in the hypothalamus by Northern hybridization. However, we could not find any specific bands in ovarian and uterine tissues. For evidence of transcription of the arginine vasotocin gene in gonadal tissues of the chicken, this study has applied the polymerase chain reaction as a highly sensitive assay. The hypothalamus, the four largest preovulatory ovarian follicles and the shell gland (uterus) were collected at 4 h and 20 h before oviposition. The ovarian follicular tissues were separated into granulosa theca interns and theca extern a layers. The uterine tissues were separated into myometrium and endometrium The extracted mRNA was converted to cDNA by reverse-transcriptase using oligo-d(T)15 primer. Then, the cDNA was amplified by Vent polymerase and arginine vasotocin specific primers. The amplification reaction was incubated by 30 cycles successively, 95°C, 55°C and 72°C earth for 1 min. The comparisons of the mRNA levels encoding arginine vasotocin between the tissues were determined by semi-quantification methods. After amplification of the cDNA, the PCR products were detected in hypothalamus, ovarian tissues and uterine tissues. The results of semi-quantification showed that the levels of arginine vasotocin mRNA in ovarian iud uterine tissues were about from 1/50 to 1/1000 when compared to that in the hypothalamus. The very low levels of mRNA encoding arginine vasotocin in ovarian and uterine tissues probably led us to conclude that arginine vasotocin may play a role of local mediate acting autocrine and/or paracrine.

Original languageEnglish
Pages (from-to)695-701
Number of pages7
JournalAsian-Australasian Journal of Animal Sciences
Volume12
Issue number5
Publication statusPublished - Aug 1999
Externally publishedYes

Fingerprint

arginine vasotocin
Vasotocin
uterine tissue
Chickens
chickens
Gene Expression
hypothalamus
gene expression
Hypothalamus
Messenger RNA
Complementary DNA
shell gland
myometrium
RNA-directed DNA polymerase
endometrium
Posterior Pituitary Hormones
ovarian follicles
Polymerase Chain Reaction
radioimmunoassays
Oviposition

Keywords

  • Arginine Vasotocin
  • Chicken
  • Ovary
  • RT-PCR
  • Uterus

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

Gene Expression of Arginine Vasotocin in Ovarian and Uterine Tissues of the Chicken. / Saito, Noboru; Grossmann, R.

In: Asian-Australasian Journal of Animal Sciences, Vol. 12, No. 5, 08.1999, p. 695-701.

Research output: Contribution to journalArticle

@article{2cdf74e78631498a88e7bd81be72b229,
title = "Gene Expression of Arginine Vasotocin in Ovarian and Uterine Tissues of the Chicken",
abstract = "The hypothalamus is the classic site of synthesis of arginine vasotocin as neurohypophyseal hormone in the chicken. However, high concentrations of arginine vasotocin were also measured in ovarian tissues by radioimmunoassay. At first, we observed specific positive signal of rnRNA encoding AVT in the hypothalamus by Northern hybridization. However, we could not find any specific bands in ovarian and uterine tissues. For evidence of transcription of the arginine vasotocin gene in gonadal tissues of the chicken, this study has applied the polymerase chain reaction as a highly sensitive assay. The hypothalamus, the four largest preovulatory ovarian follicles and the shell gland (uterus) were collected at 4 h and 20 h before oviposition. The ovarian follicular tissues were separated into granulosa theca interns and theca extern a layers. The uterine tissues were separated into myometrium and endometrium The extracted mRNA was converted to cDNA by reverse-transcriptase using oligo-d(T)15 primer. Then, the cDNA was amplified by Vent polymerase and arginine vasotocin specific primers. The amplification reaction was incubated by 30 cycles successively, 95°C, 55°C and 72°C earth for 1 min. The comparisons of the mRNA levels encoding arginine vasotocin between the tissues were determined by semi-quantification methods. After amplification of the cDNA, the PCR products were detected in hypothalamus, ovarian tissues and uterine tissues. The results of semi-quantification showed that the levels of arginine vasotocin mRNA in ovarian iud uterine tissues were about from 1/50 to 1/1000 when compared to that in the hypothalamus. The very low levels of mRNA encoding arginine vasotocin in ovarian and uterine tissues probably led us to conclude that arginine vasotocin may play a role of local mediate acting autocrine and/or paracrine.",
keywords = "Arginine Vasotocin, Chicken, Ovary, RT-PCR, Uterus",
author = "Noboru Saito and R. Grossmann",
year = "1999",
month = "8",
language = "English",
volume = "12",
pages = "695--701",
journal = "Asian-Australasian Journal of Animal Sciences",
issn = "1011-2367",
publisher = "Asian-Australasian Association of Animal Production Societies",
number = "5",

}

TY - JOUR

T1 - Gene Expression of Arginine Vasotocin in Ovarian and Uterine Tissues of the Chicken

AU - Saito, Noboru

AU - Grossmann, R.

PY - 1999/8

Y1 - 1999/8

N2 - The hypothalamus is the classic site of synthesis of arginine vasotocin as neurohypophyseal hormone in the chicken. However, high concentrations of arginine vasotocin were also measured in ovarian tissues by radioimmunoassay. At first, we observed specific positive signal of rnRNA encoding AVT in the hypothalamus by Northern hybridization. However, we could not find any specific bands in ovarian and uterine tissues. For evidence of transcription of the arginine vasotocin gene in gonadal tissues of the chicken, this study has applied the polymerase chain reaction as a highly sensitive assay. The hypothalamus, the four largest preovulatory ovarian follicles and the shell gland (uterus) were collected at 4 h and 20 h before oviposition. The ovarian follicular tissues were separated into granulosa theca interns and theca extern a layers. The uterine tissues were separated into myometrium and endometrium The extracted mRNA was converted to cDNA by reverse-transcriptase using oligo-d(T)15 primer. Then, the cDNA was amplified by Vent polymerase and arginine vasotocin specific primers. The amplification reaction was incubated by 30 cycles successively, 95°C, 55°C and 72°C earth for 1 min. The comparisons of the mRNA levels encoding arginine vasotocin between the tissues were determined by semi-quantification methods. After amplification of the cDNA, the PCR products were detected in hypothalamus, ovarian tissues and uterine tissues. The results of semi-quantification showed that the levels of arginine vasotocin mRNA in ovarian iud uterine tissues were about from 1/50 to 1/1000 when compared to that in the hypothalamus. The very low levels of mRNA encoding arginine vasotocin in ovarian and uterine tissues probably led us to conclude that arginine vasotocin may play a role of local mediate acting autocrine and/or paracrine.

AB - The hypothalamus is the classic site of synthesis of arginine vasotocin as neurohypophyseal hormone in the chicken. However, high concentrations of arginine vasotocin were also measured in ovarian tissues by radioimmunoassay. At first, we observed specific positive signal of rnRNA encoding AVT in the hypothalamus by Northern hybridization. However, we could not find any specific bands in ovarian and uterine tissues. For evidence of transcription of the arginine vasotocin gene in gonadal tissues of the chicken, this study has applied the polymerase chain reaction as a highly sensitive assay. The hypothalamus, the four largest preovulatory ovarian follicles and the shell gland (uterus) were collected at 4 h and 20 h before oviposition. The ovarian follicular tissues were separated into granulosa theca interns and theca extern a layers. The uterine tissues were separated into myometrium and endometrium The extracted mRNA was converted to cDNA by reverse-transcriptase using oligo-d(T)15 primer. Then, the cDNA was amplified by Vent polymerase and arginine vasotocin specific primers. The amplification reaction was incubated by 30 cycles successively, 95°C, 55°C and 72°C earth for 1 min. The comparisons of the mRNA levels encoding arginine vasotocin between the tissues were determined by semi-quantification methods. After amplification of the cDNA, the PCR products were detected in hypothalamus, ovarian tissues and uterine tissues. The results of semi-quantification showed that the levels of arginine vasotocin mRNA in ovarian iud uterine tissues were about from 1/50 to 1/1000 when compared to that in the hypothalamus. The very low levels of mRNA encoding arginine vasotocin in ovarian and uterine tissues probably led us to conclude that arginine vasotocin may play a role of local mediate acting autocrine and/or paracrine.

KW - Arginine Vasotocin

KW - Chicken

KW - Ovary

KW - RT-PCR

KW - Uterus

UR - http://www.scopus.com/inward/record.url?scp=0033408738&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033408738&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:0033408738

VL - 12

SP - 695

EP - 701

JO - Asian-Australasian Journal of Animal Sciences

JF - Asian-Australasian Journal of Animal Sciences

SN - 1011-2367

IS - 5

ER -